Multi-chamber single-use bioreactor– A proof of concept prototype

Abstract Background: A need for improved, cassette-based automation of 61Cu separation from irradiated Ni targets was identified given the growing interest in theranostics and generally lengthy separation chemistries for 64Cu/64Ni, upon which 61Cu chemistry is often based. Methods: A method for separating 61Cu from irradiated natNi targets was therefore developed, with provision for target recycling. Following deuteron irradiation, electroplated natNi targets were remotely transferred from the cyclotron and dissolved in acid. The dissolved target solution was then transferred to an automated FASTlab chemistry module, where sequential TBP and TK201 (Triskem) resins isolated the [61Cu]CuCl2, removed Ni, Co, and Fe, and concentrated the product into a formulation suitable for anticipated radiolabelling reactions. Results: 61Cu saturation yields of 190 ± 33 MBq/µA from energetically thick natNi targets were measured. The average, decay-corrected, activity-based dissolution efficiency was 97.5 ± 1.4 % with an average radiochemical yield of 90.4 ± 3.2 % (N = 5). The isolated activity was collected approximately 65 minutes post end of bombardment in ~2 mL of 0.06 M HCl (HCl concentration was verified by titration). Quality control of the isolated [61Cu]CuCl2 (N = 5) measured 58Co content of (8.3 ± 0.6) × 10-5 % vs. 61Cu by activity, Ni separation factors ≥ (2.2 ± 1.8) × 106, EoB molar activities 85 ± 23 GBq/µmol and NOTA-based EoB apparent molar activities of 31 ± 8 MBq/nmol and 201 MBq/nmol for the 30 min and 3.3 h (N = 1) irradiations, respectively. Conclusion: High purity 61Cu was produced with the developed automated method using a single-use, cassette-based approach. It was also applicable for 64Cu, as demonstrated with a single proof-of-concept 64Ni target production run.

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Expansion of Adipose Derived Stromal Cells from Stromal Vascular Fraction in a Single-Use Bioreactor: Proof of Concept in the Mobius® 3L

Cytotherapy ◽

10.1016/j.jcyt.2016.03.267 ◽

2016 ◽

Vol 18 (6) ◽

pp. S138

Author(s):

M. Gadelorge ◽

M. Bourdens ◽

C. Bardiaux ◽

N. Espagnolle ◽

J. Murrell ◽

...

Keyword(s):

Stromal Cells ◽

Stromal Vascular Fraction ◽

Proof Of Concept ◽

Adipose Derived Stromal Cells ◽

Single Use

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Design, prototyping, and evaluation of a collapsible device for single-operator sheathing of ultrasound probes

Mechanical Sciences ◽

10.5194/ms-5-7-2014 ◽

2014 ◽

Vol 5 (1) ◽

pp. 7-13

Author(s):

S. A. Lopez ◽

L. R. Hernley ◽

E. N. Bearrick ◽

L. M. Tanenbaum ◽

M. A. C. Thomas ◽

...

Keyword(s):

Interventional Ultrasound ◽

Proof Of Concept ◽

Ultrasound Guided ◽

Single Operator ◽

Single Use ◽

Single User ◽

Probe Holder ◽

Ultrasound Probes

Abstract. During interventional ultrasound-guided procedures, sterility is maintained by covering the transducer head and cord with a sterile sheath. The current sheathing technique is cumbersome, requires an assistant to complete, and poses a risk of tangling the probe cord and breaching the sterile barrier. This paper presents the design, proof-of-concept prototyping, and evaluation of a probe holder and cartridge-style, single-use applicator that enables faster, more reliable, single-user sheathing of ultrasound probes, with a decreased risk of compromising sterility.

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Automated, cassette-based isolation and formulation of high-purity [61Cu]CuCl2 from solid Ni targets

EJNMMI Radiopharmacy and Chemistry ◽

10.1186/s41181-020-00108-7 ◽

2020 ◽

Vol 5 (1) ◽

Author(s):

Johan Svedjehed ◽

Christopher J. Kutyreff ◽

Jonathan W. Engle ◽

Katherine Gagnon

Keyword(s):

High Purity ◽

Radiochemical Yield ◽

Proof Of Concept ◽

Automated Method ◽

Production Run ◽

Separation Factors ◽

Single Use ◽

Target Production ◽

Dissolution Efficiency ◽

Hcl Concentration

Abstract Background A need for improved, cassette-based automation of 61Cu separation from irradiated Ni targets was identified given the growing interest in theranostics, and generally lengthy separation chemistries for 64Cu/64Ni, upon which 61Cu chemistry is often based. Methods A method for separating 61Cu from irradiated natNi targets was therefore developed, with provision for target recycling. Following deuteron irradiation, electroplated natNi targets were remotely transferred from the cyclotron and dissolved in acid. The dissolved target solution was then transferred to an automated FASTlab chemistry module, where sequential TBP and TK201 (Triskem) resins isolated the [61Cu]CuCl2, removed Ni, Co, and Fe, and concentrated the product into a formulation suitable for anticipated radiolabelling reactions. Results 61Cu saturation yields of 190 ± 33 MBq/μA from energetically thick natNi targets were measured. The average, decay-corrected, activity-based dissolution efficiency was 97.5 ± 1.4% with an average radiochemical yield of 90.4 ± 3.2% (N = 5). The isolated activity was collected approximately 65 min post end of bombardment in ~ 2 mL of 0.06 M HCl (HCl concentration was verified by titration). Quality control of the isolated [61Cu]CuCl2 (N = 5) measured 58Co content of (8.3 ± 0.6) × 10− 5% vs. 61Cu by activity, Ni separation factors ≥ (2.2 ± 1.8) × 106, EoB molar activities 85 ± 23 GBq/μmol and NOTA-based EoB apparent molar activities of 31 ± 8 MBq/nmol and 201 MBq/nmol for the 30 min and 3.3 h (N = 1) irradiations, respectively. Conclusion High purity 61Cu was produced with the developed automated method using a single-use, cassette-based approach. It was also applicable for 64Cu, as demonstrated with a single proof-of-concept 64Ni target production run.

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Automated, Cassette-Based Isolation and Formulation of High-Purity [61Cu]CuCl2 from Solid Ni Targets

10.21203/rs.3.rs-41194/v1 ◽

2020 ◽

Author(s):

Johan Pettersson ◽

Christopher J Kutyreff ◽

Jonathan W Engle ◽

Katherine Gagnon

Keyword(s):

High Purity ◽

Radiochemical Yield ◽

Proof Of Concept ◽

Automated Method ◽

Production Run ◽

Separation Factors ◽

Single Use ◽

Target Production ◽

Dissolution Efficiency ◽

Hcl Concentration

Abstract Background: A need for improved, cassette-based automation of 61Cu separation from irradiated Ni targets was identified given the growing interest in theranostics and generally lengthy separation chemistries for 64Cu/ 64Ni, upon which 61Cu chemistry is often based.Methods: A method for separating 61Cu from irradiated natNi targets was therefore developed, with provision for target recycling. Following deuteron irradiation, electroplated natNi targets were remotely transferred from the cyclotron and dissolved in acid. The dissolved target solution was then transferred to an automated FASTlab chemistry module, where sequential TBP and TK201 (Triskem) resins isolated the [ 61Cu]CuCl2 , removed Ni, Co, and Fe, and concentrated the product into a formulation suitable for anticipated radiolabelling reactions.Results: 61Cu saturation yields of 190 ± 33 MBq/µA from energetically thick natNi targets were measured. The average, decay-corrected, activity-based dissolution efficiency was 97.5 ± 1.4 % with an average radiochemical yield of 90.4 ± 3.2 % (N = 5). The isolated activity was collected approximately 65 minutes post end of bombardment in ~2 mL of 0.06 M HCl (HCl concentration was verified by titration). Quality control of the isolated [61Cu]CuCl 2 (N = 5) measured 58Co content of (8.3 ± 0.6) × 10-5 % vs. 61Cu by activity, Ni separation factors ≥(2.2 ± 1.8) × 106 , EoB molar activities 85 ± 23 GBq/µmol and NOTA-based EoB apparent molar activities of 31 ± 8 MBq/nmol and 201 MBq/nmol for the 30 min and 3.3 h (N = 1) irradiations, respectively.Conclusion: High purity 61Cu was produced with the developed automated method using a single-use, cassette-based approach. It was also applicable for 64Cu, as demonstrated with a single proof-of-concept 64Ni target production run.

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Clinical-scale expansion of adipose-derived stromal cells starting from stromal vascular fraction in a single-use bioreactor: proof of concept for autologous applications

Journal of Tissue Engineering and Regenerative Medicine ◽

10.1002/term.2377 ◽

2017 ◽

Vol 12 (1) ◽

pp. 129-141 ◽

Cited By ~ 6

Author(s):

Mélanie Gadelorge ◽

Marion Bourdens ◽

Nicolas Espagnolle ◽

Clémence Bardiaux ◽

Julie Murrell ◽

...

Keyword(s):

Stromal Cells ◽

Stromal Vascular Fraction ◽

Clinical Scale ◽

Proof Of Concept ◽

Adipose Derived Stromal Cells ◽

Single Use ◽

Scale Expansion

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COVID-19 Face Masks as a Long-Term Source of Microplastics in Recycled Urban Green Waste

Sustainability ◽

10.3390/su14010207 ◽

2021 ◽

Vol 14 (1) ◽

pp. 207

Author(s):

Dirk H. R. Spennemann

Keyword(s):

Tensile Strength ◽

Uv Light ◽

Municipal Waste ◽

Proof Of Concept ◽

Urban Green ◽

Green Waste ◽

Lawn Mower ◽

Cutting Equipment ◽

Single Use

Following the outbreak of the COVID-19 pandemic in March 2020, many governments recommended or mandated the wearing of fitted face masks to limit the transmission of the SARS-CoV-2 virus via aerosols. Concomitant with the extensive use of non-sterile, surgical-type single-use face masks (SUM) was an increase of such masks, either lost or discarded, in various environmental settings. With their low tensile strength, the spunbond and melt-blown fabrics of the SUM are prone to shredding into small pieces when impacted by lawn cutting equipment. Observations highlight the absence of smaller pieces, which are either wind-dispersed or collected by the mower’s leaf catcher and disposed together with the green waste and then enter the municipal waste stream. As proof-of-concept, experiments using a domestic lawn-mower with different height settings and different grass heights, show that 75% of all pieces of SUM fabric caught in the catcher belonged to sizes below 10 mm2, which under the influence of UV light will decay into microfibers. The implications of SUM generated microplastics are discussed.

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Direct measurement of electron-diffraction-pattern intensities using an energy loss spectrometer

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100155311 ◽

1989 ◽

Vol 47 ◽

pp. 668-669

Author(s):

A. G. Jackson ◽

M. Rowe

Keyword(s):

Thermal Effects ◽

Dynamic Range ◽

Scattering Amplitude ◽

Dynamical Theory ◽

Site Symmetry ◽

Proof Of Concept ◽

Parallel Acquisition ◽

Kinematic Approximation ◽

Speed Up ◽

Structure Calculations

Diffraction intensities from intermetallic compounds are, in the kinematic approximation, proportional to the scattering amplitude from the element doing the scattering. More detailed calculations have shown that site symmetry and occupation by various atom species also affects the intensity in a diffracted beam. [1] Hence, by measuring the intensities of beams, or their ratios, the occupancy can be estimated. Measurement of the intensity values also allows structure calculations to be made to determine the spatial distribution of the potentials doing the scattering. Thermal effects are also present as a background contribution. Inelastic effects such as loss or absorption/excitation complicate the intensity behavior, and dynamical theory is required to estimate the intensity value.The dynamic range of currents in diffracted beams can be 104or 105:1. Hence, detection of such information requires a means for collecting the intensity over a signal-to-noise range beyond that obtainable with a single film plate, which has a S/N of about 103:1. Although such a collection system is not available currently, a simple system consisting of instrumentation on an existing STEM can be used as a proof of concept which has a S/N of about 255:1, limited by the 8 bit pixel attributes used in the electronics. Use of 24 bit pixel attributes would easily allowthe desired noise range to be attained in the processing instrumentation. The S/N of the scintillator used by the photoelectron sensor is about 106 to 1, well beyond the S/N goal. The trade-off that must be made is the time for acquiring the signal, since the pattern can be obtained in seconds using film plates, compared to 10 to 20 minutes for a pattern to be acquired using the digital scan. Parallel acquisition would, of course, speed up this process immensely.

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