Kinetic analysis and evaluation of galactose oxidase activation by hematin in the green oxidation of glycerol

2021 ◽  
pp. 108203
Author(s):  
Adrián Parodi ◽  
Mariano Asteasuain ◽  
Ivana Magario
Keyword(s):  
Kinetic Analysis ◽  
Galactose Oxidase ◽  
Analysis And Evaluation ◽  
Green Oxidation

Green Oxidation Catalysts: Computational Design of High-Efficiency Models of Galactose Oxidase

2004 ◽  
Vol 43 (25) ◽  
pp. 3286-3289 ◽  
Author(s):  
Leonardo Guidoni ◽  
Katrin Spiegel ◽  
Martin Zumstein ◽  
Ursula Röthlisberger
Keyword(s):  
High Efficiency ◽  
Computational Design ◽  
Galactose Oxidase ◽  
Oxidation Catalysts ◽  
Green Oxidation

Green Oxidation Catalysts: Computational Design of High-Efficiency Models of Galactose Oxidase

2004 ◽  
Vol 116 (25) ◽  
pp. 3348-3351 ◽  
Author(s):  
Leonardo Guidoni ◽  
Katrin Spiegel ◽  
Martin Zumstein ◽  
Ursula Röthlisberger
Keyword(s):  
High Efficiency ◽  
Computational Design ◽  
Galactose Oxidase ◽  
Oxidation Catalysts ◽  
Green Oxidation

Kinetic Analysis and Evaluation of Controlled Release ofD-Limonene Encapsulated in Spray-Dried Cyclodextrin Powder under Linearly Ramped Humidity

2012 ◽  
Vol 30 (11-12) ◽  
pp. 1283-1291 ◽  
Author(s):  
Chisho Yamamoto ◽  
Tze Loon Neoh ◽  
Hirokazu Honbou ◽  
Hidefumi Yoshii ◽  
Takeshi Furuta
Keyword(s):  
Controlled Release ◽  
Kinetic Analysis ◽  
Analysis And Evaluation ◽  
Spray Dried

Upper gastrointestinal endoscopic ultrasonography analysis and evaluation of clinical epidemiology

Endoscopy ◽  
2011 ◽  
Vol 43 (S 03) ◽  
Author(s):  
Zheng-xiang Wu ◽  
Ming-li Zhang ◽  
Zuo Wang ◽  
Kai-guang Zhang ◽  
Xi-ping Ding ◽  
...  
Keyword(s):  
Endoscopic Ultrasonography ◽  
Clinical Epidemiology ◽  
Analysis And Evaluation ◽  
Upper Gastrointestinal

Functional Fractionation of Platelets: Aggregation Kinetics and Glycoprotein Labeling of Differing Platelet Populations

1982 ◽  
Vol 48 (02) ◽  
pp. 211-216 ◽  
Author(s):  
V M Haver ◽  
A R L Gear
Keyword(s):  
Galactose Oxidase ◽  
Aggregation Kinetics ◽  
Maximal Rate ◽  
Membrane Glycoproteins ◽  
Whole Cells ◽  
Reversible Aggregation ◽  
Significant Difference ◽  
Small Doses ◽  
Major Loss ◽  
Kinetics Of

SummaryPlatelet heterogeneity has been studied with a technique called functional fractionation which employs gentle centrifugation to yield subpopulations (“reactive” and “less-reactive” platelets) after exposure to small doses of aggregating agent. Aggregation kinetics of the different platelet populations were investigated by quenched-flow aggregometry. The large, “reactive” platelets were more sensitive to ADP (Ka = 1.74 μM) than the smaller “less-reactive” platelets (Ka = 4.08 μM). However, their maximal rate of aggregation (Vmax, % of platelets aggregating per sec) of 23.3 was significantly lower than the “less-reactive” platelets (Vmax = 34.7). The “reactive” platelets had a 2.2 fold higher level of cyclic AMP.Platelet glycoproteins were labeled using the neuraminidase-galactose oxidase – [H3]-NaBH4 technique. When platelets were labeled after reversible aggregation, the “reactive” platelets showed a two-fold decrease in labeling efficiency (versus control platelets). However, examination of whole cells or membrane preparations from reversibly aggregated platelets revealed no significant difference in Coomassie or PAS (Schiff) staining.These results suggest that the large, “reactive” platelets are more sensitive to ADP but are not hyperaggregable in a kinetic sense. Reversible aggregation may cause a re-orientation of membrane glycoproteins that is apparently not characterized by a major loss of glycoprotein material.

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

1965 ◽  
Vol 13 (01) ◽  
pp. 155-175 ◽  
Author(s):  
H. C Hemker ◽  
P.W Hemker ◽  
E. A Loeliger
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Kinetics ◽  
Blood Clotting ◽  
Enzyme System ◽  
Enzyme Kinetic ◽  
Clotting Time ◽  
Standard Methods

SummaryApplication of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :1. Substrate is not present in excess.2. Only relative measures exist for concentrations of substrate or enzymes.3. Enzymes and substrates are often added together.4. Reagents are not pure.5. Clotting-time is our only measure for clotting-velocity.Formulas are deduced, which makes it possible to recognize the effect of these complications.

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