Influence of temperature on the insecticidal effect of Beauveria bassiana in combination with diatomaceous earth against Rhyzopertha dominica and Sitophilus oryzae on stored wheat

2006 ◽  
Vol 38 (2) ◽  
pp. 270-281 ◽  
Author(s):  
T.N. Vassilakos ◽  
C.G. Athanassiou ◽  
N.G. Kavallieratos ◽  
B.J. Vayias
1997 ◽  
Vol 26 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Alcides Moino Jr. ◽  
Sérgio B. Alves

Determinou-se a suscetibilidade de Sitophilus oryzae (L.), S. zeamais (Motsch.) e Rhyzopertha dominica (Fabr.) a dois isolados do fungo Beauveria bassiana (Bals.) Vuill. Para isso foram usados recipientes contendo arroz beneficiado, inoculado previamente com conídios dos isolados, infestados com as três espécies de insetos. Esses recipientes foram mantidos a 26 ± 0,5°C, 70 ± 10% de UR e fotofase de 12 horas. As três espécies foram suscetíveis aos dois isolados do patógeno. Os dois isolados foram eficientes para o controle de S. oryzae, na concentração de 0,5 g de conídios/100 g de grãos. Para S. zeamais, o isolado 604 foi mais eficiente na concentração de 0,1 g de conídios/100 g de grãos. Com relação a R. dominica, foram suficientes dosagens de 0,01 g (isolado 476) e 0,05 g (isolado 604)/100 g de grãos, confirmando a maior suscetibilidade desta espécie ao patógeno.


Insects ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 613 ◽  
Author(s):  
Ivan Paponja ◽  
Vlatka Rozman ◽  
Anita Liška

Diatomaceous earth (DE) has long been known as a potential protectant for stored cereals against various stored product insects. Despite favorable effect for the environment and human health, DE has some negative side effects on the treated commodity. In order to minimize negative response and to improve its efficacy, this paper represents a study of developed natural formulation based on DE SilicoSec® enhanced with botanicals (essential oil lavender, corn oil, and bay leaves dust) and silica gel. The activity of formulation (labeled as N Form) was tested against Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Tribolium castaneum (Herbst) in seed wheat and barley under controlled conditions. As a reference comparative value, DE SilicoSec® was used. N Form showed higher efficacy than DE, especially in barley at the lowest concentration, inducing higher mortality of all three insect species. The highest average progeny inhibition was recorded in R. dominica population both in seed wheat and barley with 94.9% and 96.3% of inhibition, respectively, followed with S. oryzae and T. castaneum inhibition of 90.6% and 86.1%, respectively, in wheat and 94.9% and 89.7%, respectively, in barley. Results indicate that the developed natural formulation N Form enhanced the activity of DE SilicoSec® using lower amount of DE dust and that it could be successfully implemented for storage of cereals as alternatives to chemical pesticides for stored product insect control.


1987 ◽  
Vol 27 (2) ◽  
pp. 309 ◽  
Author(s):  
JM Desmarchelier ◽  
JC Dines

The efficacy of Dryacide (diatomaceous earth coated with silica aerogels) on wheat was tested in the laboratory against adult and immature stages of 4 species of Coleoptera and against immature stages of 1 species of Lepidoptera. The minimum effective level of Dryacide increased in the following order: immature Ephestia cautella (Walker) ~ immature Tribolium castaneum (Herbst) < immature Rhyzopertha dominica (F) < adult R. dominica ~ adult T. castaneum < adult Sitophilus oryzae (L) < adult Sitophilus granarius (L) < immature Sitophilus species. When wheat treated with Dryacide was milled in the laboratory without prior cleaning, less than 3% of the Dryacide carried over into the flour. Commercial cleaning of wheat removed (�s.e.) about 98 � 1% of Dryacide, and no Dryacide could be detected in the flour. Dryacide treatment did not affect flour quality as determined by the volume of sponge cakes and the production of carbon dioxide by fermenting dough.


2020 ◽  
Vol 10 (18) ◽  
pp. 6441
Author(s):  
Georgia V. Baliota ◽  
Christos G. Athanassiou

Laboratory bioassays were conducted to evaluate the insecticidal efficacy of a diatomaceous earth deposit from Greece, for a wide range of stored product insects. In this context, populations of five different insect species, Tribolium confusum Jacquelin DuVal, the confused flour beetle; Sitophilus oryzae (L.), the rice weevil; Rhyzopertha dominica (F.), the lesser grain borer; Oryzaephilus surinamensis (L.), the sawtoothed grain beetle; Cryptolestes ferrugineus (Stephens), the rusty grain beetle, which cover a major spectrum of insects species of stored products worldwide, were used in the bioassays. The different treatment of diatomaceous earth (DE) rocks (grinding, diatomaceous enrichment, powder granulometry) led to the creation of five types of formulations (namely DE1, DE2, DE3, DE5 and DE6) that exhibited significant fluctuations in their insecticidal efficacy when applied on wheat. In general, some of the modified formulations were found to be very effective against species such as R. dominica and T. confusum that may be difficult to control at the current labeled doses of commercial DE formulations. Overall, our data clearly indicate that this specific Greek deposit has considerable insecticidal properties, which can be further utilized in designing commercial formulations for insect control at the postharvest stages of durable agricultural commodities, provided that the deposit will be modified at specific enrichment and granulometry levels.


Author(s):  
Yacoub Ahmad Batta

The present research is aimed at a formulation of the entomopathogenic fungi, Beauveria bassiana (Bal.) Vuillemin and Metarhizium anisopliae (Metch.) Sorokin, in two types of diatomaceous earth dusts, fossil shield and Silico-Sec, are then applied against the adults of three species of stored-grain insects: Sitophilus oryzae L., Rhyzopertha dominica Fab. and Tribolium castaneum Herbs. Effect of the treatment was assessed by comparing the mortality percentage of the adults of the three insect species exposed to the formulated fungi with that of the adults exposed to the unformulated fungi or the diatomaceous earth dusts or the undisturbed control. Results obtained from these exposures have indicated that treatment of the adults with the formulated fungi resulted in a significantly higher mean mortality percentage compared to the treatment with the unformulated fungi or the diatomaceous earth dusts or the undisturbed control. A synergistic interaction between the effect of fungal species and the diatomaceous earth dusts was shown. Viability of conidia of both fungal species in diatomaceous earth dusts was assessed by calculating the germination percentage of the conidia over time. Results indicated a small loss of mean germination percentage for formulated conidia of both fungal species versus a high loss of mean germination percentage for the unformulated conidia, thus the diatomaceous earth dusts used in the formulation of both fungi demonstrated a negligible effect on the viability of formulated conidia compared to the unformulated.


1980 ◽  
Vol 58 (9) ◽  
pp. 1524-1534 ◽  
Author(s):  
N. D. G. White ◽  
R. N. Sinha

The consequences of infestation of bulk-stored wheat by multiple species of insects were determined for 60 weeks at 30 ± 2 °C. Eight 204-L drums containing wheat at 15.5% moisture content were used as three distinct systems: (I) Control system (two drums), insect free; (II) RST system (three drums), infested with the grouping of Rhyzopertha dominica F., Sitophilus oryzae (L.), and Tribolium castaneum (Herbst); and (III) COT system (three drums), infested with the grouping of Cryptolestes ferrugineus (Stephens), Oryzaephilus surinamensis (L.), and T. castaneum. At triweekly intervals carbon dioxide, oxygen, temperature, grain moisture, seed damage, grain weight, dust weight, fat acidity values (FAV), seed germination, microflora, and the numbers of insects and the mite Tarsonemus granarius Lindquist were measured. Seeds died by week 15 and bacterial infection on them increased in all systems. The seed FAVs in the RST system peaked by week 30 and then declined steadily while these values were increasing continuously in the Control and levelling off in the COT systems. Insects multiplied exponentially for 6–15 weeks and then declined sharply or maintained oscillating populations; Sitophilus and Oryzaephilus were unable to survive in the presence of the other insects.


2011 ◽  
Vol 41 (6) ◽  
pp. 233-241 ◽  
Author(s):  
Waqas WAKIL ◽  
Tahira RIASAT ◽  
M. Usman GHAZANFAR ◽  
Yong Jung KWON ◽  
Farid Asif SHAHEEN

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