Analysis of grain yield and flour quality of some winter wheat varieties in the 2020 production

Pšenica, je tijekom cijele ljudske civilizacije bila bazni i vitalni sastojak svakodnevne ishrane ljudi i domaćih životinja. U Republici Srbiji posljednjih nekoliko godina izražena je traženost sorti pšenice, koje pored visokog i stabilnog prinosa imaju i dobre parametre kvalitete brašna. U provedenim istraživanjima u 2019/2020. godini sorta s najstabilnijim prinosom i dobrom kvalitetom zrna pokazala se sorta Nogal. Ova sorta ima visok sadržaj kvalitetnih proteina, a analogno tome je i visok sadržaj glutena, ali i kvaliteta, što su potvrdila reološka mjerenja. Ostvareni visoki prinosi zrna ispitivanih sorata pšenice jesu rezultat interakcije genetičkog potencijala ispitivanih sorata pšenice i primjene dobre agrotehnike u uvjetima kada raspored padalina u tijeku vegetacijske sezone, od momenta sjetve, pa do žetve nije pogodovao proizvodnji pšenice. Istraživanja su pokazala da se sorte Talas, Pobeda i Nogal mogu svrstati u kvalitetnu grupu A2, odnosno to su sorte dobre kvalitete brašna i hljeba i kao takve spadaju u grupu poboljšivača. Sorte Merkur, Imperija, Ratarica i Vizantija svrstane su u kvalitetnu grupu B1 tj. one su dobre hljebne sorte. Sorta NS 40 S svrstana je u kvalitetnu grupu B2.

Significance of Sodium Bisulfate (SBS) Tempering in Reducing the Escherichia coli O121 and O26 Load of Wheat and Its Effects on Wheat Flour Quality

The occurrence of recalls involving pathogenic Escherichia coli-contaminated wheat flours show the need for incorporating antimicrobial interventions in wheat milling. The objectives of this study were to assess the efficacy of sodium bisulfate (SBS) tempering in reducing E. coli O121 (ATCC 2219) and O26 (ATCC 2196) wheat load and to evaluate the impact of effective (≥3.0 log reductions) SBS treatments on wheat flour quality. Wheat grains were inoculated with E. coli (~6 log CFU/g) and tempered (17% moisture, 24 h) using the following SBS concentrations (%wheat basis): 0, 0.5, 0.75, 1.0, 1.25, and 1.5% SBS. Reductions in E. coli O121 and O26 wheat load at different time intervals (0.5, 2, 6, 12, 18, and 24 h) during tempering were evaluated. The addition of SBS during tempering resulted in E. coli (O121 and O26) log reductions of 2.0 (0.5% SBS) to >4.0 logs (1.5% SBS) (p ≤ 0.05). SBS tempering (1.25 and 1.5% SBS) produced acidic wheat flours (pH = 4.51–4.60) but had comparable wheat flour properties in terms of composition, dough, and bread-making properties relative to the control (0% SBS). SBS tempering reduced the E. coli O121 and O26 load of wheat after tempering with minimal effects on wheat flour quality.

Post-Translational Cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum)

Wheat is a major staple food crop worldwide because of the unique properties of wheat flour. High molecular weight glutenin subunits (HMW-GSs), which are among the most critical determinants of wheat flour quality, are responsible for the formation of glutenin polymeric structures via interchain disulfide bonds. We herein describe the identification of a new HMW-GS Dy10 allele (Dy10-m619SN). The amino acid substitution (serine-to-asparagine) encoded in this allele resulted in a partial post-translational cleavage that produced two new peptides. These new peptides disrupted the interactions among gluten proteins because of the associated changes to the number of available cysteine residues for interchain disulfide bonds. Consequently, Dy10-m619SN expression decreased the size of glutenin polymers and weakened glutens, which resulted in wheat dough with improved cookie-making quality, without changes to the glutenin-to-gliadin ratio. In this study, we clarified the post-translational processing of HMW-GSs and revealed a new genetic resource useful for wheat breeding.