The effect of biological pretreatment with the selective white-rot fungus Echinodontium taxodii on enzymatic hydrolysis of softwoods and hardwoods

2009 ◽  
Vol 100 (21) ◽  
pp. 5170-5175 ◽  
Author(s):  
Hongbo Yu ◽  
Guoning Guo ◽  
Xiaoyu Zhang ◽  
Keliang Yan ◽  
Chunyan Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
White Rot ◽  
White Rot Fungus ◽  
Biological Pretreatment ◽  
Hydrolysis Of

scholarly journals Biological Pretreatment of Rubberwood withCeriporiopsis subvermisporafor Enzymatic Hydrolysis and Bioethanol Production

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Forough Nazarpour ◽  
Dzulkefly Kuang Abdullah ◽  
Norhafizah Abdullah ◽  
Nazila Motedayen ◽  
Reza Zamiri
Keyword(s):  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Raw Material ◽  
White Rot ◽  
White Rot Fungus ◽  
Cellulose Content ◽  
Bioethanol Production ◽  
Biological Pretreatment ◽  
Spectra Analysis ◽  
Alternative Material

Rubberwood (Hevea brasiliensis), a potential raw material for bioethanol production due to its high cellulose content, was used as a novel feedstock for enzymatic hydrolysis and bioethanol production using biological pretreatment. To improve ethanol production, rubberwood was pretreated with white rot fungusCeriporiopsis subvermisporato increase fermentation efficiency. The effects of particle size of rubberwood (1 mm, 0.5 mm, and 0.25 mm) and pretreatment time on the biological pretreatment were first determined by chemical analysis and X-ray diffraction and their best condition obtained with 1 mm particle size and 90 days pretreatment. Further morphological study on rubberwood with 1 mm particle size pretreated by fungus was performed by FT-IR spectra analysis and SEM observation and the result indicated the ability of this fungus for pretreatment. A study on enzymatic hydrolysis resulted in an increased sugar yield of 27.67% as compared with untreated rubberwood (2.88%). The maximum ethanol concentration and yield were 17.9 g/L and 53% yield, respectively, after 120 hours. The results obtained demonstrate that rubberwood pretreated byC. subvermisporacan be used as an alternative material for the enzymatic hydrolysis and bioethanol production.

scholarly journals Biological pretreatment of wheat straw: Effect of fungal culturing on enzymatic hydrolysis of carbohydrate polymers

2021 ◽  
Author(s):  
Aleksandar Knežević ◽  
Ivana Đokić ◽  
Tomislav Tosti ◽  
Slađana Popović ◽  
Dušanka Milojković-Opsenica ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Lignin Degradation ◽  
Enzymatic Saccharification ◽  
White Rot Fungi ◽  
Fungal Species ◽  
White Rot ◽  
Irpex Lacteus ◽  
Fungal Pretreatment ◽  
Hydrolysis Of

Abstract The aim of the study was comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Cyclocybe cylindracea, Ganoderma resinaceum, Irpex lacteus, Pleurotus ostreatus and Trametes versicolor were the species studied. Peroxidases were predominantly responsible for lignin degradation even though high laccase activities were detected, except in the case of Irpex lacteus where laccase activity was not detected. Studied fungal species showed various ability to degrade lignin in wheat straw which further affected release of reducing sugars during enzymatic saccharification. The highest rate of lignin degradation was noticed in sample pretreated with Irpex lacteus (50.9 ± 4.1%). Among all tested species only Ganoderma resinaceum was suitable lignin degrader with the 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control, and could have significant biotechnological application due to lower cellulose loss. A key mechanism of carbohydrate component convertibility enhancement was lignin removal in the biomass. Long time consumption, the low sugar yields and unpredictable fungal response still remain the challenge of the fungal pretreatment process.

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