scholarly journals Biological pretreatment of wheat straw: Effect of fungal culturing on enzymatic hydrolysis of carbohydrate polymers

2021 ◽  
Author(s):  
Aleksandar Knežević ◽  
Ivana Đokić ◽  
Tomislav Tosti ◽  
Slađana Popović ◽  
Dušanka Milojković-Opsenica ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Lignin Degradation ◽  
Enzymatic Saccharification ◽  
White Rot Fungi ◽  
Fungal Species ◽  
White Rot ◽  
Irpex Lacteus ◽  
Fungal Pretreatment ◽  
Hydrolysis Of

Abstract The aim of the study was comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Cyclocybe cylindracea, Ganoderma resinaceum, Irpex lacteus, Pleurotus ostreatus and Trametes versicolor were the species studied. Peroxidases were predominantly responsible for lignin degradation even though high laccase activities were detected, except in the case of Irpex lacteus where laccase activity was not detected. Studied fungal species showed various ability to degrade lignin in wheat straw which further affected release of reducing sugars during enzymatic saccharification. The highest rate of lignin degradation was noticed in sample pretreated with Irpex lacteus (50.9 ± 4.1%). Among all tested species only Ganoderma resinaceum was suitable lignin degrader with the 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control, and could have significant biotechnological application due to lower cellulose loss. A key mechanism of carbohydrate component convertibility enhancement was lignin removal in the biomass. Long time consumption, the low sugar yields and unpredictable fungal response still remain the challenge of the fungal pretreatment process.

scholarly journals Enzymatic saccharification of biologically pre-treated wheat straw with white-rot fungi

2010 ◽  
Vol 101 (15) ◽  
pp. 6045-6050 ◽  
Author(s):  
Albino A. Dias ◽  
Gil S. Freitas ◽  
Guilhermina S.M. Marques ◽  
Ana Sampaio ◽  
Irene S. Fraga ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Enzymatic Saccharification ◽  
White Rot Fungi ◽  
White Rot

scholarly journals Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment

2013 ◽  
Vol 6 (1) ◽  
pp. 115 ◽  
Author(s):  
Davinia Salvachúa ◽  
Angel T Martínez ◽  
Ming Tien ◽  
María F López-Lucendo ◽  
Francisco García ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Proteomic Analysis ◽  
White Rot Fungi ◽  
White Rot ◽  
Irpex Lacteus

Comparison of Low-Temperature Alkali/Urea Pretreatments for Ethanol Production from Wheat Straw

2021 ◽  
Vol 15 (3) ◽  
pp. 399-407
Author(s):  
Zahoor ◽  
Wen Wang ◽  
Xuesong Tan ◽  
Qiang Yu ◽  
Yongming Sun ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Ethanol Production ◽  
Wheat Straw ◽  
Response Surface ◽  
Cellulosic Ethanol ◽  
Enzymatic Saccharification ◽  
Hydrolysis Of ◽  
Bioethanol Yield

NaOH/urea (NU) pretreatment at lower than 0 °C has been frequently applied for improving bio-conversion of lignocellulose, but the wastewater generated from the pretreatment process is hard to dispose. KOH/urea (KU) pretreatment for enhancing bioconversion of lignocellulose has recently attracted researchers’ attention due to the recycling of wastewater for facilitating crops’ growth. This study compared the effects of NU and KU pretreatments at cold conditions on the enzymatic hydrolysis and bioethanol yield from wheat straw (WS). By using response surface methodology an optimal pretreatment with an equal ratio of alkali/urea (4% w/v) at −20 °C for 3 h was established. The enzymatic hydrolysis of KU-treated WS was 81.17%, which was similar to that of NU-treated WS (83.72%) under the same condition. It means that KU pretreatment has equal ability to NU pretreatment to improve enzymatic saccharification of lignocellulose. KU pretreatment has the promising potential to replace NU pretreatment for facilitating bioconversion of lignocellulose in cold conditions due to the clean way to recycle its wastewater as fertilizer for crop growth. Hence, KU pretreatment combined with enzymatic hydrolysis and fermentation could be a promising green way to cellulosic ethanol production with zero waste emission.

scholarly journals Production of xylooligosaccharides from enzymatic hydrolysis of xylan by the white-rot fungi Pleurotus

2009 ◽  
Vol 63 (6) ◽  
pp. 673-678 ◽  
Author(s):  
Cristiano Ragagnin de Menezes ◽  
Ísis Serrano Silva ◽  
Érika Cristina Pavarina ◽  
Elisângela Franciscon Guímaro Dias ◽  
Fábio Guímaro Dias ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
White Rot Fungi ◽  
White Rot ◽  
Hydrolysis Of

scholarly journals Production of xylooligosaccharides from enzymatic hydrolysis of xylan by white-rot fungi Pleurotus

2010 ◽  
Vol 32 (1) ◽  
Author(s):  
Cristiano Ragagnin de Menezes ◽  
Ísis Serrano Silva ◽  
Érika Cristina Pavarina ◽  
Andréia Fonseca de Faria ◽  
Elisângela Franciscon ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
White Rot Fungi ◽  
White Rot ◽  
Hydrolysis Of

scholarly journals The remediation of chlorpyrifos-contaminated soil by immobilized white-rot fungi

2020 ◽  
Vol 85 (7) ◽  
pp. 857-868
Author(s):  
Xin Wang ◽  
Lei Song ◽  
Zhaoxing Li ◽  
Zijun Ni ◽  
Jia Bao ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Corn Stover ◽  
Contaminated Soils ◽  
Ph Value ◽  
Orthogonal Experiment ◽  
Wood Chip ◽  
White Rot Fungi ◽  
White Rot ◽  
Irpex Lacteus ◽  
Biomass Dosage

This research focused on the degradation of chlorpyrifos via immobilized white rot fungi in soil, with the aim to select excellent degrading strains and an optimal carrier of white rot fungi. Immobilization of white rot fungi was assessed on corn stover, wheat straw, peanut shells, wood chip, and corn cobs. Phlebia sp., Lenzites betulinus and Irpex lacteus were grown in defined nutrient media for the remediation of pesticide-contaminated soils. The carrier of the biomass was determined by observing the growth of white rot fungi. The results showed that corn stover and wheat straw are suitable carriers of the immobilized white rot fungi and that Phlebia sp. and Lenzites betulinus have a positive effect on the degradation of chlorpyrifos. At 30?C and neutral pH, the degradation rate of chlorpyrifos was 74.35 %, Phlebia sp. being immobilized by corn stover in 7 days, which was the best result compared to other combinations of strains and carriers. The orthogonal experiment showed that the pH value and temperature affected the pollutant degradability more than the initial concentration and the biomass dosage.

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