Salinity manipulation as an effective method for enhanced starch production in the marine microalga Tetraselmis subcordiformis

2013 ◽  
Vol 146 ◽  
pp. 663-671 ◽  
Author(s):  
Chang-Hong Yao ◽  
Jiang-Ning Ai ◽  
Xu-Peng Cao ◽  
Song Xue
Keyword(s):  
Tetraselmis Subcordiformis ◽  
Marine Microalga ◽  
Starch Production

scholarly journals Recombinant Expression of Thrombolytic Agent Reteplase in Marine Microalga Tetraselmis subcordiformis (Chlorodendrales, Chlorophyta)

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 315
Author(s):  
Chunhui Wu ◽  
Caiyun Zheng ◽  
Jinxia Wang ◽  
Peng Jiang
Keyword(s):  
Thrombolytic Agent ◽  
Recombinant Expression ◽  
Penaeid Shrimp ◽  
Tissue Type ◽  
Nuclear Transformation ◽  
Tetraselmis Subcordiformis ◽  
Cell Factories ◽  
Marine Microalga ◽  
Type Plasminogen Activator ◽  
Expression Product

Tetraselmis subcordiformis, a unicellular marine green alga, is used widely in aquaculture as an initial feeding for fish, bivalve mollusks, penaeid shrimp larvae, and rotifers because of its rich content of amino acids and fatty acids. A stable nuclear transformation system using the herbicide phosphinothricin (PPT) as a selective reagent was established previously. In this research, the recombinant expression in T. subcordiformis was investigated by particle bombardment with the rt-PA gene that encodes the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction treatment. Transgenic algal strains were selected by their resistance to PPT, and expression of rt-PA was validated by PCR, Southern blotting, and Western blotting, and bioactivity of rt-PA was confirmed by the fibrin agarose plate assay for bioactivity. The results showed that rt-PA was integrated into the genome of T. subcordiformis, and the expression product was bioactive, indicating proper post-transcriptional modification of rt-PA in T. subcordiformis. This report contributes to efforts that take advantage of marine microalgae as cell factories to prepare recombinant drugs and in establishing a characteristic pathway of oral administration in aquaculture.

Comparative transcriptional study on the hydrogen evolution of marine microalga Tetraselmis subcordiformis

2014 ◽  
Vol 39 (32) ◽  
pp. 18235-18246 ◽  
Author(s):  
Xupeng Cao ◽  
Xudong Wu ◽  
Chaofan Ji ◽  
Changhong Yao ◽  
Zhaoan Chen ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
Tetraselmis Subcordiformis ◽  
Marine Microalga

Enhancing starch production of a marine green microalga Tetraselmis subcordiformis through nutrient limitation

2012 ◽  
Vol 118 ◽  
pp. 438-444 ◽  
Author(s):  
Changhong Yao ◽  
Jiangning Ai ◽  
Xupeng Cao ◽  
Song Xue ◽  
Wei Zhang
Keyword(s):  
Nutrient Limitation ◽  
Tetraselmis Subcordiformis ◽  
Green Microalga ◽  
Starch Production
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