Waste in islands: a political ecology perspective on postcolonial metabolism

Islands are tightly connected to globalized material flows, with specific constraints and vulnerabilities. They are not closed metabolic loops of consumption, production, and waste, favorable to the circular economy. Small islands allow the observation of the material outcomes of circulation, from overflowing dumpsites to marine debris washing up on the shore. We argue that islands are key territories for better understanding the Capitalocene, precisely because of the ways in which they are connected to (rather than isolated from) globalized material flows. This article is a comparative geographical analysis of waste realities in three French/formerly French island territories: Ndzuwani (Comoros), Réunion, and New Caledonia. It builds on metabolism analysis and waste studies—in particular waste colonialism—to address the different perspectives that these approaches open up for the study of island territories. The long-term sociohistorical context of each island helps to explain contemporary waste management policies and practices. A material flow analysis makes it possible to sketch out metabolic profiles that show the contribution of prevailing mining and agricultural industries to waste generation. The comparison of current situations regarding household waste discourses and economies shows how these territories are characterized by waste accumulation.

Accumulation of Anthocyanidins Determines Leaf Color of Liquidambar Formosana as Revealed by Transcriptome Sequencing and Metabolism Analysis

Liquidambar formosana is important for its ornamental value in China; it is increasingly used for landscaping and gardening trees due to its diverse leaf colors and seasonal changes. Varieties including either a fixed leaf color, the purplish ‘Fuluzifeng’ (ZF), or seasonal changes in leaf color, the reddish ‘Nanlinhong’ (NLH) have been bred and registered as new plant varieties under the International Union for the Protection of New Plant Varieties (UPOV) system. To gain practical insights into the anthocyanin biosynthetic process, transcriptome sequencing (Illumina) was performed to clarify the metabolic pathways present in the three seasonal changes in leaf colors in NLH and in the springtime purple-red color of ZF. qRT-PCR was used to verify the speculation. Based on the differentially expressed genes and flavonoids analyses, the spring, summer, and autumn leaves of NLH were compared to study the seasonal differences. NLH and ZF were compared to study the formation mechanism of the two leaf colors in spring. Transcriptome sequencing produced a total of 121,216 unigenes from all samples, where 48 unigenes were differentially expressed and associated with the anthocyanidin pathway. The expression levels of LfDFR and LfANS genes corresponded to the accumulation of concentrations of cyanidins in spring (NLHC) and autumn leaves (NLHQ), respectively, with different shades of red. Moreover, the LfF3′5′H gene corresponded to the accumulation of flavonols and delphinidins in purple-red leaves (ZFC). Cyanidin and peonidin were the key pigments in red and dark-red leaves, and purple-red leaves were co-pigmented by cyanidins, pelargonidins, and delphinidins.

The Chromosome-Level Genome of Miracle Fruit (Synsepalum dulcificum) Provides New Insights Into the Evolution and Function of Miraculin

Miracle fruit (Synsepalum dulcificum) is a rare valuable tropical plant famous for a miraculous sweetening glycoprotein, miraculin, which can modify sour flavors to sweet flavors tasted by humans. Here, we present a chromosome-level high-quality genome of S. dulcificum with an assembly genome size of ∼550 Mb, contig N50 of ∼14.14 Mb, and 37,911 annotated protein-coding genes. Phylogenetic analysis revealed that S. dulcificum was most closely related to Camellia sinensis and Diospyros oleifera, and that S. dulcificum diverged from the Diospyros genus ∼75.8 million years ago (MYA), and that C. sinensis diverged from Synsepalum ∼63.5 MYA. Ks assessment and collinearity analysis with S. dulcificum and other species suggested that a whole-genome duplication (WGD) event occurred in S. dulcificum and that there was good collinearity between S. dulcificum and Vitis vinifera. On the other hand, transcriptome and metabolism analysis with six tissues containing three developmental stages of fleshes and seeds of miracle fruit revealed that Gene Ontology (GO) terms and metabolic pathways of “cellular response to chitin,” “plant–pathogen interaction,” and “plant hormone signal transduction” were significantly enriched during fruit development. Interestingly, the expression of miraculin (Chr10G0299340) progressively increased from vegetative organs to reproductive organs and reached an incredible level in mature fruit flesh, with an fragments per kilobase of transcript per million (FPKM) value of ∼113,515, which was the most highly expressed gene among all detected genes. Combining the unique signal peptide and the presence of the histidine-30 residue together composed the main potential factors impacting miraculin’s unique properties in S. dulcificum. Furthermore, integrated analysis of weighted gene coexpression network analysis (WGCNA), enrichment and metabolite correlation suggested that miraculin plays potential roles in regulating plant growth, seed germination and maturation, resisting pathogen infection, and environmental pressure. In summary, valuable genomic, transcriptomic, and metabolic resources provided in this study will promote the utilization of S. dulcificum and in-depth research on species in the Sapotaceae family.

Investigation of PtSGT1 and PtSGT4 Function in Cellulose Biosynthesis in Populus tomentosa Using CRISPR/Cas9 Technology

Cellulose synthesis is a complex process in plant cells that is important for wood processing, pulping, and papermaking. Cellulose synthesis begins with the glycosylation of sitosterol by sitosterol glycosyltransferase (SGT) to produce sitosterol-glucoside (SG), which acts as the guiding primer for cellulose production. However, the biological functions of SGTs in Populus tomentosa (P. tomentosa) remain largely unknown. Two full-length PtSGT genes (PtSGT1 and PtSGT4) were previously isolated from P. tomentosa and characterized. In the present study, CRISPR/Cas9 gene-editing technology was used to construct PtSGT1-sgRNA and PtSGT4-sgRNA expression vectors, which were genetically transformed into P. tomentosa using the Agrobacterium-mediated method to obtain transgenic lines. Nucleic acid and amino acid sequencing analysis revealed both base insertions and deletions, in addition to reading frame shifts and early termination of translation in the transgenic lines. Sugar metabolism analysis indicated that sucrose and fructose were significantly downregulated in stems and leaves of mutant PtSGT1-1 and PtSGT4-1. Glucose levels did not change significantly in roots and stems of PtSGT1-1 mutants; however, glucose was significantly upregulated in stems and downregulated in leaves of the PtSGT4-1 mutants. Dissection of the plants revealed disordered and loosely arranged xylem cells in the PtSGT4-1 mutant, which were larger and thinner than those of the wild-type. This work will enhance our understanding of cellulose synthesis in the cell walls of woody plants.

Phosphorus Governance within Planetary Boundaries: The Potential of Strategic Local Resource Planning in The Hague and Delfland, The Netherlands

In recent years, a growing body of research has explored the urban dimension of the critical resource phosphorus with a focus on urban metabolism analysis, recovery technologies and governance frameworks. However, there has been no tangible and holistic attempt at choosing between available measures and instruments for their implementation in the urban realm. With the growing and increasingly urban world population, cities have become more and more important as actors in phosphorus governance by fueling global phosphorus flows, e.g., via the consumption of food, agricultural products, and phosphorus recycling. Globally, a future-proof phosphorus metabolism may be contributed to by strategic local phosphorus planning. This article systematically explores the purposive potential of local phosphorus planning using a case study of The Hague, The Netherlands. Looking across multiple administrative and spatial dimensions, the study combines quantitative analysis of phosphorus flows with qualitative investigation of their drivers, the actors involved, current regulations and local scope for action. The research reveals the feasibility of meaningfully impacting phosphorus flows through urban action. The potential for strategic local resource planning is demonstrated in a grid of policy options and in the assessment of their socio-economic and environmental benefits. Additionally, the study draws up a list of key recommendations to transfer to other urban settings. It encourages further research aimed at closing data gaps for local phosphorus inventories, collaborative approaches in strategic resource planning, scope for action in other cities or jurisdictions, as well as improved quantification of the outreach of policy effects.