Combined pretreatment using ozonolysis and ball milling to improve enzymatic saccharification of corn straw

Sugarcane bagasse is the major by-product of the sugarcane industry and, due to its abundant availability, it has been extensively studied for lignocellulosic bioconversion in the production of bioethanol and other value-added commercial products. In the study presented herein, a combined pretreatment using sulfolane, TiO2 and alkali microwave irradiation (MW-A) was assessed for the dissolution of lignin prior to enzymatic saccharification of holocellulose. Total reducing sugars (TRS) and saccharinic acid yields were investigated. The increase in NaOH concentration up to 5% and in temperature from 120 °C to 140 °C were found to have a positive influence on both yields. While increasing the reaction time from 5 to 60 min only led to an increase in TRS yield <2%, a reaction time of 30 min almost doubled the saccharinic acids production. TRS yields and saccharinic acid production were approximately 5% and 33% higher when the sulfolane-TiO2 reaction medium was used, as compared to MW-A in water, reaching up to 64.8% and 15.24 g/L of saccharinic acids, respectively. The proposed MW-A pretreatment may hold promise for industrial applications, given the good TRS yields obtained, and the associated enzyme and time/energy savings. The use of sulfolane-TiO2 reaction medium is encouraged if saccharinic acids are to be recovered too.

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Combined pretreatment using ozonolysis and wet-disk milling to improve enzymatic saccharification of Japanese cedar

Bioresource Technology ◽

10.1016/j.biortech.2012.09.030 ◽

2012 ◽

Vol 126 ◽

pp. 182-186 ◽

Cited By ~ 38

Author(s):

Toyokazu Miura ◽

Seung-Hwan Lee ◽

Seiichi Inoue ◽

Takashi Endo

Keyword(s):

Enzymatic Saccharification ◽

Japanese Cedar ◽

Combined Pretreatment

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Process intensification effect of ball milling on the hydrothermal pretreatment for corn straw enzymolysis

Energy Conversion and Management ◽

10.1016/j.enconman.2015.05.057 ◽

2015 ◽

Vol 101 ◽

pp. 481-488 ◽

Cited By ~ 35

Author(s):

Zhengqiu Yuan ◽

Jinxing Long ◽

Tiejun Wang ◽

Riyang Shu ◽

Qi Zhang ◽

...

Keyword(s):

Ball Milling ◽

Process Intensification ◽

Hydrothermal Pretreatment ◽

Corn Straw

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Efficiency of Corn and Poplar Biomass Saccharification after Pretreatment with Potassium Hydroxide

Ecological Chemistry and Engineering S ◽

10.2478/eces-2020-0002 ◽

2020 ◽

Vol 27 (1) ◽

pp. 41-53

Author(s):

Katarzyna Dąbkowska-Susfał

Keyword(s):

Lignocellulosic Biomass ◽

Potassium Hydroxide ◽

Enzymatic Saccharification ◽

Biomass Composition ◽

Poplar Wood ◽

Corn Straw ◽

Biomass Saccharification ◽

Biomass Processing ◽

Lignocellulosic Feedstocks ◽

Pretreatment Time

AbstractPretreatment is an essential step in the conversion of lignocellulosic biomass into valuable products. It aims to increase the biomass susceptibility to enzymatic saccharification to generate fermentable monosaccharides. In this study, the efficiency of 2 % potassium hydroxide (KOH) solution used as a pretreating agent for various lignocellulosic feedstocks, such as corn straw, corncob, and poplar wood, was evaluated. The influence of the pretreatment time, which varied from 0.5 to 24 h at 50 °C, on the alteration of biomass composition was investigated, as well as the enzymatic digestibility. Finally, the overall sugar yields were determined. For corncob, the yield on average amounted to 453.9 ±18.9 mg·g−1 raw (untreated) biomass, regardless of the pretreatment time. The overall sugar yield for both the corn straw and poplar wood biomass increased with increased pretreatment time and ranged from 333.0 to 438.4 mg·g−1 raw biomass and from 123.2 to 215.7 mg·g−1 raw biomass, respectively. Based on the results obtained, the most appropriate pretreatment times for all types of biomass were proposed. The results of this study may be useful for the development of lignocellulosic biomass processing technology.

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