A cost-practical cell-recycling process for xylonic acid bioproduction from acidic lignocellulosic hydrolysate with whole-cell catalysis of Gluconobacter oxydans

2021 ◽  
Vol 333 ◽  
pp. 125157
Author(s):  
Jian Han ◽  
Xia Hua ◽  
Xin Zhou ◽  
Bin Xu ◽  
Huan Wang ◽  
...  
Keyword(s):  
Gluconobacter Oxydans ◽  
Lignocellulosic Hydrolysate ◽  
Recycling Process ◽  
Whole Cell ◽  
Xylonic Acid ◽  
Cell Recycling ◽  
Whole Cell Catalysis

scholarly journals Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS)

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Xia Hua ◽  
Xin Zhou ◽  
GenLai Du ◽  
Yong Xu
Keyword(s):  
Oxygen Supply ◽  
Chemical Engineering ◽  
Gluconobacter Oxydans ◽  
Critical Issue ◽  
High Density ◽  
Chemical Production ◽  
Oxygen Utilization ◽  
Whole Cell ◽  
Xylonic Acid ◽  
Whole Cell Catalysis

Abstract Background The critical issue in the competitiveness between bioengineering and chemical engineering is the products titer and the volume productivity. The most direct and effective approach usually employs high-density biocatalyst, while the weakened mass transfer and evoked foam problem accompany ultrahigh-density biocatalyst loading and substrate/product titer. In high-density obligate aerobic bioconversion, oxygen as electron acceptor is a speed-limiting step in bioprocesses, but sufficient oxygen supply will lead to the foaming which results in a significant reduction in oxygen utilization and the use of additional defoamers. In this study, we designed a novel sealed-oxygen supply (SOS) biotechnology to resolve the formidable barrier of oxygen transferring rate (OTR), for bio-based fuels and chemical production process. Results Based on systemic analysis of whole-cell catalysis in Gluconobacter oxydans, a novel sealed-oxygen supply technology was smartly designed and experimentally performed for biocatalytic oxidation of alcohols, sugars and so on. By a simple operation skill of automatic online supply of oxygen in a sealed stirring tank bioreactor of SOS, OTR barrier and foaming problem was resolved with great ease. We finally obtained ultrahigh-titer products of xylonic acid (XA), 3-hydroxypropionic acid (3-HPA), and erythrulose at 588.4 g/L, 69.4 g/L, and 364.7 g/L, respectively. Moreover, the volume productivity of three chemical products was improved by 150–250% compared with normal biotechnology. This SOS technology provides a promising approach to promote bioengineering competitiveness and advantages over chemical engineering. Conclusion SOS technology was demonstrated as an economic and universally applicable approach to bio-based fuels and chemicals production by whole-cell catalysis. The novel technology greatly promotes the competitiveness of bioengineering for chemical engineering, and provides a promising platform for the green and environmental use of biofuels.

scholarly journals A techno-practical method for overcoming the biotoxicity and volatility obstacles of butanol and butyric acid during whole-cell catalysis by Gluconobacter oxydans

2020 ◽  
Author(s):  
Xia Hua ◽  
GenLai Du ◽  
Xin Zhou ◽  
Nawaz Ali ◽  
Haq ul Ikram ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Industrial Production ◽  
Gluconobacter Oxydans ◽  
Practical Method ◽  
Environmental Benefits ◽  
Utilization Rate ◽  
Diverse Range ◽  
Whole Cell ◽  
Whole Cell Catalysis ◽  
Butyric Acid Production

Abstract Background: Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality butyric acid used in food and medicine, is more dependent on microbiological production methods. Hence, the bio-oxidation of butanol to butyric acid has been identified as a promising method with good potential economic and environmental benefits. However, both butanol and butyric acid usually performs intensive toxicity on most microorganisms as well as the bio-oxidation pathway. To develop a green, efficient and competitive microbiological method is the primary work to overcome the bottleneck of butyric acid industry. Result: A combined bioprocess was designed with alternative whole-cell catalysis for butyric acid bio-conversion from butanol by Gluconobacter oxydans in a sealed-oxygen supply bioreactor (SOS). In the operation system, the escape of volatile substrates and toxic chemicals to cells can be avoided by the use of a sealed bioreactor, combined with the rejuvenation of cells by supplying energy co-factors. Finally, during one batch whole-cell catalysis, the utilization rate of substrate increased from 56.6% to 96.0% by the simply skill. Additionally, the techno-practical bioprocess can realize the purpose of cell-recycling technology through the rejuvenation effect of co-factor. Finally, we obtained 135.3 g/L butyric acid and 216.7 g/L sorbose during 60 h whole-cell catalysis. This techno-practical technology provides a promising approach to promote the industrial production of butyric acid with more competitiveness. Conclusion: The techno-practical biotechnology has powerfully promoted the process of butyric acid production by microorganism, especially makes up for the lack of aerobic fermentation in the industry, and surmount the shortcomings of traditional anerobic fermentation. At the same time, this technically-practical system provides a promising approach for the promotion of the industrial production of butyric acid in a more competitive manner.

scholarly journals A techno-practical method for overcoming the biotoxicity and volatility obstacles of butanol and butyric acid during whole-cell catalysis by Gluconobacter oxydans

2020 ◽  
Author(s):  
Xia Hua ◽  
GenLai Du ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Butyric Acid ◽  
Industrial Production ◽  
Gluconobacter Oxydans ◽  
Practical Method ◽  
Environmental Benefits ◽  
Utilization Rate ◽  
Diverse Range ◽  
Whole Cell ◽  
Whole Cell Catalysis ◽  
Butyric Acid Production

Abstract Background Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality butyric acid used in food and medicine, is more dependent on microbiological production methods. Hence, the bio-oxidation of butanol to butyric acid has been identified as a promising method with good potential economic and environmental benefits. However, both butanol and butyric acid usually performs intensive toxicity on most microorganisms as well as the bio-oxidation pathway. To develop a green, efficient and competitive microbiological method is the primary work to overcome the bottleneck of butyric acid industry. Result A combined bioprocess was designed with alternative whole-cell catalysis for butyric acid bio-conversion from butanol by Gluconobacter oxydans in a sealed-oxygen supply bioreactor (SOS). In the operation system, the escape of volatile substrates and toxic chemicals to cells can be avoided by the use of a sealed bioreactor, combined with the rejuvenation of cells by supplying energy co-factors. Finally, during one batch whole-cell catalysis, the utilization rate of substrate increased from 56.6% to 96.0% by the simply skill. Additionally, the techno-practical bioprocess can realize the purpose of cell-recycling technology through the rejuvenation effect of co-factor. Finally, we obtained 135.3 g/L butyric acid and 216.7 g/L sorbose during 60 h whole-cell catalysis. This techno-practical technology provides a promising approach to promote the industrial production of butyric acid with more competitiveness. Conclusion The techno-practical biotechnology has powerfully promoted the process of butyric acid production by microorganism, especially makes up for the lack of aerobic fermentation in the industry, and surmount the shortcomings of traditional anerobic fermentation. At the same time, this technically-practical system provides a promising approach for the promotion of the industrial production of butyric acid in a more competitive manner.

scholarly journals Characteristics and Kinetics of the Aldonic Acids Production using Whole-cell catalysis of Gluconobacter oxydans

BioResources ◽  
2015 ◽  
Vol 10 (3) ◽  
Author(s):  
Xin Zhou ◽  
Xing Wang ◽  
Rou Cao ◽  
Yuheng Tao ◽  
Yong Xu ◽  
...  
Keyword(s):  
Gluconobacter Oxydans ◽  
Whole Cell ◽  
Whole Cell Catalysis ◽  
Kinetics Of

Workflows for optimization of enzyme cascades and whole cell catalysis based on enzyme kinetic characterization and pathway modelling

2022 ◽  
Vol 74 ◽  
pp. 55-60
Author(s):  
Laura Kuschmierz ◽  
Lu Shen ◽  
Christopher Bräsen ◽  
Jacky Snoep ◽  
Bettina Siebers
Keyword(s):  
Enzyme Kinetic ◽  
Kinetic Characterization ◽  
Whole Cell ◽  
Whole Cell Catalysis ◽  
Enzyme Cascades
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