Alkaline pretreatment and response surface methodology based recombinant enzymatic saccharification and fermentation of sugarcane tops

2021 ◽  
Vol 341 ◽  
pp. 125837
Author(s):  
Kaustubh Chandrakant Khaire ◽  
Vijayanand Suryakant Moholkar ◽  
Arun Goyal
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzymatic Saccharification ◽  
Alkaline Pretreatment

Physicochemical characterization of alkali pretreated sugarcane tops and optimization of enzymatic saccharification using response surface methodology

2014 ◽  
Vol 62 ◽  
pp. 362-368 ◽  
Author(s):  
Raveendran Sindhu ◽  
Mathiyazhakan Kuttiraja ◽  
Parameswaran Binod ◽  
Rajeev Kumar Sukumaran ◽  
Ashok Pandey
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Physicochemical Characterization ◽  
Enzymatic Saccharification

Comparison of Low-Temperature Alkali/Urea Pretreatments for Ethanol Production from Wheat Straw

2021 ◽  
Vol 15 (3) ◽  
pp. 399-407
Author(s):  
Zahoor ◽  
Wen Wang ◽  
Xuesong Tan ◽  
Qiang Yu ◽  
Yongming Sun ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Ethanol Production ◽  
Wheat Straw ◽  
Response Surface ◽  
Cellulosic Ethanol ◽  
Enzymatic Saccharification ◽  
Hydrolysis Of ◽  
Bioethanol Yield

NaOH/urea (NU) pretreatment at lower than 0 °C has been frequently applied for improving bio-conversion of lignocellulose, but the wastewater generated from the pretreatment process is hard to dispose. KOH/urea (KU) pretreatment for enhancing bioconversion of lignocellulose has recently attracted researchers’ attention due to the recycling of wastewater for facilitating crops’ growth. This study compared the effects of NU and KU pretreatments at cold conditions on the enzymatic hydrolysis and bioethanol yield from wheat straw (WS). By using response surface methodology an optimal pretreatment with an equal ratio of alkali/urea (4% w/v) at −20 °C for 3 h was established. The enzymatic hydrolysis of KU-treated WS was 81.17%, which was similar to that of NU-treated WS (83.72%) under the same condition. It means that KU pretreatment has equal ability to NU pretreatment to improve enzymatic saccharification of lignocellulose. KU pretreatment has the promising potential to replace NU pretreatment for facilitating bioconversion of lignocellulose in cold conditions due to the clean way to recycle its wastewater as fertilizer for crop growth. Hence, KU pretreatment combined with enzymatic hydrolysis and fermentation could be a promising green way to cellulosic ethanol production with zero waste emission.

scholarly journals Optimisation of alkaline pretreatment conditions of orange and plantain peels for polygalacturonase production by Aspergillus awamori CICC 2040

2021 ◽  
Vol 37 (2) ◽  
pp. 13-21
Author(s):  
O.E. Adedeji ◽  
O.O. Ezekiel
Keyword(s):  
Particle Size ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Alkaline Pretreatment ◽  
Aspergillus Awamori ◽  
Pretreatment Conditions ◽  
Polygalacturonase Production ◽  
Polygalacturonase Activity

This study investigated the optimisation of alkaline pretreatment of orange and plantain peels for polygalacturonase (PG) production by Aspergillus awamori CICC 2040 using response surface methodology. The factors evaluated were particle size, PS (< 0.4250, 0.4250 < PS < 0.8025 and 0.8025 < PS < 1.1800 mm), NaOH molarity (0.010, 0.055, and 0.100 M), and time (1.0, 6.5, and 12.0 h). These factors were interacted to determine the most suitable combinations for maximum polygalacturonase activity (MPA). The pretreated orange and plantain peel powders were inoculated with 106 spores/mL Aspergillus awamori CICC 2040 was incubated at 28 oC for 5 days, and crude PG was extracted and its activity determined. The alkaline pretreatment combinations that gave MPA were <0.4250 mm, 0.100 M, and 1.0 h, and 0.8025 < PS < 1.1800 mm, 0.010 M, and 1.0 h for orange and plantain peel powders, respectively. The MPA obtained from the pretreated orange and plantain peel powders were 38.46 and 38.82 U/mL, respectively. Optimised alkaline pretreatment conditions of the orange and plantain peels for MPA, produced by Aspergillus awamori CICC 2040, were established. Keywords: Aspergillus awamori CICC 2040, Peel, Optimisation, Polygalacturonase, Pretreatment

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