An assembly-regulated SNAP-tag fluorogenic probe for long-term super-resolution imaging of mitochondrial dynamics

2021 ◽  
Vol 176 ◽  
pp. 112886
Author(s):  
Wenjuan Liu ◽  
Qinglong Qiao ◽  
Jiazhu Zheng ◽  
Jie Chen ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Mitochondrial Dynamics ◽  
Super Resolution ◽  
Fluorogenic Probe ◽  
Resolution Imaging

Long-term super-resolution imaging of mitochondrial dynamics

2020 ◽  
Vol 31 (11) ◽  
pp. 2937-2940 ◽  
Author(s):  
Xiaolian Li ◽  
Jiazhu Zheng ◽  
Wenjuan Liu ◽  
Qinglong Qiao ◽  
Jie Chen ◽  
...  
Keyword(s):  
Mitochondrial Dynamics ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Fluorescence imaging with tailored light

Nanophotonics ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 2111-2128 ◽  
Author(s):  
Jialei Tang ◽  
Jinhan Ren ◽  
Kyu Young Han
Keyword(s):  
Fluorescence Imaging ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Optical Parameters ◽  
Propagation Angle ◽  
Resolution Imaging ◽  
Time Observation ◽  
Living Organisms ◽  
Real Time Observation

AbstractFluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization, and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.

Long-term STED imaging of amyloid fibers with exchangeable Thioflavin T

Nanoscale ◽  
2020 ◽  
Vol 12 (28) ◽  
pp. 15050-15053
Author(s):  
Joaquim Torra ◽  
Patricia Bondia ◽  
Sylvia Gutierrez-Erlandsson ◽  
Begoña Sot ◽  
Cristina Flors
Keyword(s):  
Super Resolution ◽  
Stimulated Emission ◽  
Thioflavin T ◽  
Amyloid Fibers ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion

We report the use of the amyloid probe Thioflavin T (ThT) as a specific and exchangeable fluorophore for stimulated emission depletion (STED) super-resolution imaging of amyloid fibers.

scholarly journals Observation of the Change of Synapses After Long-Term Potentiation Induction using Super-Resolution Imaging

2014 ◽  
Vol 106 (2) ◽  
pp. 435a
Author(s):  
Sang Hak Lee ◽  
En Cai ◽  
Okunola Jeyifous ◽  
Michelle A. Baird ◽  
Michael W. Davidson ◽  
...  
Keyword(s):  
Super Resolution ◽  
Long Term Potentiation ◽  
Term Potentiation ◽  
Resolution Imaging

scholarly journals Tiled Reconstruction Improves Structured Illumination Microscopy

2020 ◽  
Author(s):  
David P. Hoffman ◽  
Eric Betzig
Keyword(s):  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Live Cell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Resolution Imaging

AbstractStructured illumination microscopy (SIM) is widely used for fast, long-term, live-cell super-resolution imaging. However, SIM images can contain substantial artifacts if the sample does not conform to the underlying assumptions of the reconstruction algorithm. Here we describe a simple, easy to implement, process that can be combined with any reconstruction algorithm to alleviate many common SIM reconstruction artifacts and briefly discuss possible extensions.

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