A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

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Faculty Opinions recommendation of Two dimensional PNA/DNA arrays: estimating the helicity of unusual nucleic acid polymers.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1020441.234475 ◽

2004 ◽

Author(s):

Chengde Mao

Keyword(s):

Nucleic Acid ◽

Two Dimensional ◽

Dna Arrays

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DNA-based constitutional dynamic networks as functional modules for logic gates and computing circuit operations

Chemical Science ◽

10.1039/d1sc01098k ◽

2021 ◽

Vol 12 (15) ◽

pp. 5473-5483

Author(s):

Zhixin Zhou ◽

Jianbang Wang ◽

R. D. Levine ◽

Francoise Remacle ◽

Itamar Willner

Keyword(s):

Nucleic Acid ◽

Dynamic Networks ◽

Dynamic Network ◽

Logic Gates ◽

Functional Modules ◽

Logic Operations

A nucleic acid-based constitutional dynamic network (CDN) provides a single functional computational module for diverse input-guided logic operations and computing circuits.

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Visualization of drug-nucleic acid interactions at atomic resolution

Journal of Molecular Biology ◽

10.1016/0022-2836(77)90254-6 ◽

1977 ◽

Vol 114 (3) ◽

pp. 333-365 ◽

Cited By ~ 203

Author(s):

Henry M. Sobell ◽

Chun-Che Tsai ◽

Shri C. Jain ◽

Steven G. Gilbert

Keyword(s):

Nucleic Acid ◽

Atomic Resolution ◽

Nucleic Acid Interactions

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Two-dimensional electronic spectroscopy as a tool for tracking molecular conformations in DNA/RNA aggregates

Faraday Discussions ◽

10.1039/c7fd00201g ◽

2018 ◽

Vol 207 ◽

pp. 233-250 ◽

Cited By ~ 6

Author(s):

Javier Segarra-Martí ◽

Vishal K. Jaiswal ◽

Ana Julieta Pepino ◽

Angelo Giussani ◽

Artur Nenov ◽

...

Keyword(s):

Nucleic Acid ◽

Ground State ◽

Electronic Spectra ◽

Crucial Role ◽

Electronic Spectroscopy ◽

Two Dimensional ◽

Molecular Conformations ◽

Computational Strategy

A computational strategy to simulate two-dimensional electronic spectra (2DES) is introduced, which allows characterising ground state conformations of flexible nucleobase aggregates that play a crucial role in nucleic acid photochemistry.

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