Slow-binding inhibition of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase

2004 ◽  
Vol 12 (11) ◽  
pp. 2965-2972 ◽  
Author(s):  
Rémi Braga ◽  
Laurence Hecquet ◽  
Casimir Blonski
Keyword(s):  
Binding Inhibition ◽  
Kdpg Aldolase

scholarly journals Accurate quantitative determination of affinity and binding kinetics for tight binding inhibition of xanthine oxidase

2021 ◽  
Vol 139 ◽  
pp. 111664
Author(s):  
Haiyang Yang ◽  
Xueyan Li ◽  
Gang Li ◽  
Huating Huang ◽  
Wenning Yang ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Xanthine Oxidase ◽  
Tight Binding ◽  
Binding Kinetics ◽  
Binding Inhibition

scholarly journals Theoretical treatment of tight-binding inhibition of an enzyme. Ribonuclease inhibitor as special case

1988 ◽  
Vol 253 (2) ◽  
pp. 517-522 ◽  
Author(s):  
J M Fominaya ◽  
J M García-Segura ◽  
M Ferreras ◽  
J G Gavilanes
Keyword(s):  
Tight Binding ◽  
Theoretical Treatment ◽  
Inhibitor Protein ◽  
Ribonuclease Inhibitor ◽  
Rat Testis ◽  
Binding Inhibition ◽  
Different Types ◽  
Functional Consequences ◽  
Inhibitor System ◽  
Special Case

A general treatment of very tight-binding inhibition is described. It was applied to purified endogenous RNAase inhibitor from rat testis. This treatment discriminates among the different types of inhibition and allows for calculation of the inhibition parameters. When very tight-binding inhibitions are studied at similar molar concentrations of both enzyme and inhibitor, a further approach is required. This is also described and applied to the RNAase inhibitor. A Ki value of 3.2 x 10(-12) M was found for this inhibitor protein. On the basis of this result, it was considered inappropriate to classify this type of inhibitor in terms of competitive or non-competitive, as has been done for such inhibitors so far. Functional consequences of this analysis are discussed for the RNAase-RNAase inhibitor system.

scholarly journals Improved Specificity of a Multiplex Immunoassay for Quantitation of Anti-Diphtheria Toxin Antibodies with the Use of Diphtheria Toxoid

2011 ◽  
Vol 18 (7) ◽  
pp. 1183-1186 ◽  
Author(s):  
Pieter G. M. van Gageldonk ◽  
Christina von Hunolstein ◽  
Fiona R. M. van der Klis ◽  
Guy A. M. Berbers
Keyword(s):  
Diphtheria Toxin ◽  
Neutralization Test ◽  
Diphtheria Toxoid ◽  
Inhibition Assay ◽  
Nonspecific Binding ◽  
Serum Samples ◽  
Multiplex Immunoassay ◽  
Toxin Binding ◽  
Binding Inhibition ◽  
Binding Inhibition Assay

ABSTRACTA nonspecific binding of antibodies to diphtheria toxin, especially in adult serum samples, was observed in our diphtheria-tetanus-pertussis multiplex immunoassay (DTaP4 MIA). This can be significantly reduced by the use of diphtheria toxoid, achieving a good correlation with the Vero cell neutralization test and the toxin binding inhibition assay.

Carbon Isosteres of the 4-Aminopyridine Substructure of Chloroquine:  Effects on pKa, Hematin Binding, Inhibition of Hemozoin Formation, and Parasite Growth

2003 ◽  
Vol 46 (14) ◽  
pp. 3166-3169 ◽  
Author(s):  
Srinivasa R. Cheruku ◽  
Souvik Maiti ◽  
Arnulf Dorn ◽  
Bernard Scorneaux ◽  
Apurba K. Bhattacharjee ◽  
...  
Keyword(s):  
Parasite Growth ◽  
Binding Inhibition
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