hDM2 protein-binding peptides screened from stapled α-helical peptide phage display libraries with different types of staple linkers

2020 ◽  
Vol 30 (23) ◽  
pp. 127605
Author(s):  
Teerapat Anananuchatkul ◽  
Hiroshi Tsutsumi ◽  
Takayuki Miki ◽  
Hisakazu Mihara
Keyword(s):  
Protein Binding ◽  
Phage Display ◽  
Phage Display Libraries ◽  
Helical Peptide ◽  
Different Types ◽  
Peptide Phage Display ◽  
Binding Peptides

Screening and Identification of PLK1-Polo Box Binding Peptides by High-Throughput Sequencing of Phage-Selected Libraries

2019 ◽  
Vol 26 (8) ◽  
pp. 620-633 ◽  
Author(s):  
Nousheen Bibi ◽  
Hafsa Niaz ◽  
Ted Hupp ◽  
Mohammad Amjad Kamal ◽  
Sajid Rashid
Keyword(s):  
Next Generation Sequencing ◽  
Phage Display ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
B Lymphocyte ◽  
Fluorescent Protein ◽  
Next Generation ◽  
Peptide Phage Display ◽  
Binding Peptides ◽  
Generation Sequencing

Background: Human proteome contains a plethora of short linear peptide motifs that is crucial for signaling and other cellular processes. These motifs are difficult to identify due to lack of systematic approach for their detection. Objective: Here we demonstrate the use of peptide phage display in combination with high throughput next generation sequencing to identify enriched peptide sequences through biopanning process against polo box domain (PBD) of mitotic polo like kinase 1 (Plk1). Methods: Purified recombinant Plk1 and two unrelated controls namely B-lymphocyte antigen (CD20) and fluorescent protein (mCherry) were subjected to peptide phage display analysis. Bacterially-propagated phage DNA was amplified by PCR using triplet bar coded primers to tag the pool from each amplicon. Results: Proteomic peptide phage display along with next generation sequencing and Bioinformatics analysis demonstrated several known and putative novel interactions which were potentially related to Plk1-PBD. With our strategy, we were able to identify and characterize several Plk1-PBD binding peptides, as well as define more precisely, consensus sequences. Conclusion: We believe that this information could provide valuable tools for exploring novel interaction involved in Plk1 signaling as well as to choose peptides for Plk1 specific drug development.

Selection Dynamic ofEscherichia coliHost in M13 Combinatorial Peptide Phage Display Libraries

2011 ◽  
Vol 75 (4) ◽  
pp. 812-815 ◽  
Author(s):  
Stefano ZANCONATO ◽  
Giovanni MINERVINI ◽  
Irene POLI ◽  
Davide De LUCREZIA
Keyword(s):  
Phage Display ◽  
Phage Display Libraries ◽  
Peptide Phage Display

Mimotopes for lupus-derived anti-DNA and nucleosome-specific autoantibodies selected from random peptide phage display libraries: facts and follies

2005 ◽  
Vol 296 (1-2) ◽  
pp. 83-93 ◽  
Author(s):  
Jürgen W. Dieker ◽  
Yong-Jiang Sun ◽  
Cor W. Jacobs ◽  
Chaim Putterman ◽  
Marc Monestier ◽  
...  
Keyword(s):  
Phage Display ◽  
Random Peptide ◽  
Phage Display Libraries ◽  
Peptide Phage Display

scholarly journals Mapping sites of interaction of p47-phox and flavocytochrome b with random-sequence peptide phage display libraries.

1995 ◽  
Vol 92 (15) ◽  
pp. 7110-7114 ◽  
Author(s):  
F. R. DeLeo ◽  
L. Yu ◽  
J. B. Burritt ◽  
L. R. Loetterle ◽  
C. W. Bond ◽  
...  
Keyword(s):  
Phage Display ◽  
Random Sequence ◽  
Phage Display Libraries ◽  
Peptide Phage Display ◽  
Flavocytochrome B

Integrin-Binding Peptides Derived from Phage Display Libraries

2003 ◽  
pp. 3-18 ◽  
Author(s):  
Erkki Koivunen ◽  
Bradley H. Restel ◽  
Daniel Rajotte ◽  
Johanna Lahdenranta ◽  
Martin Hagedorn ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Display Libraries ◽  
Binding Peptides
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