A novel method for lateral callus distraction and its importance for the mechano-biology of bone formation

Abstract Titanium is a highly biocompatible material and very osteogenic in vivo. However, how titanium regulates osteoblast activity to promote bone formation is incompletely characterized. We, therefore, attempted to get more information by using microRNA (miRNA) microarray techniques to investigate translation regulation in osteoblasts grown on titanium disks. The miRNA oligonucleotide microarray provides a novel method to carry out genome-wide miRNA profiling in human samples. By using miRNA microarrays containing 329 probes designed from the human miRNA sequence, several miRNA were identified in osteoblast-like cell line (MG 63) grown on titanium disks. There were 13 up-regulated miRNAs (ie, mir-23a, mir-222, mir-523, mir-22, mir-23b, mir-143, mir-377, mir-24, mir-422b, mir-26a, mir-29a, mir-17–5p, mir-182) and 2 down-regulated miRNAs (ie, mir-187, mir-339). The data reported are, to our knowledge, the first study on translation regulation in osteoblasts exposed to titanium. The data can be relevant to understand better the molecular mechanism of osteoblast activation and as a model for comparing other materials with similar clinical effects.

Download Full-text

Intramembranous bone formation after callus distraction is augmented by increasing axial compressive strain

PLoS ONE ◽

10.1371/journal.pone.0195466 ◽

2018 ◽

Vol 13 (4) ◽

pp. e0195466 ◽

Cited By ~ 3

Author(s):

Julian Schuelke ◽

Nicholaus Meyers ◽

Sandra Reitmaier ◽

Svenja Klose ◽

Anita Ignatius ◽

...

Keyword(s):

Bone Formation ◽

Compressive Strain ◽

Callus Distraction ◽

Intramembranous Bone ◽

Intramembranous Bone Formation

Download Full-text

A novel method to assess subchondral bone formation using [18F]NaF-PET in the evaluation of knee degeneration

Nuclear Medicine Communications ◽

10.1097/mnm.0000000000000819 ◽

2018 ◽

Vol 39 (5) ◽

pp. 451-456 ◽

Cited By ~ 7

Author(s):

Venkata S. Jonnakuti ◽

William Y. Raynor ◽

Elena Taratuta ◽

Thomas J. Werner ◽

Abass Alavi ◽

...

Keyword(s):

Bone Formation ◽

Subchondral Bone ◽

Novel Method

Download Full-text

The mode of interfragmentary movement affects bone formation and revascularization after callus distraction

PLoS ONE ◽

10.1371/journal.pone.0202702 ◽

2018 ◽

Vol 13 (8) ◽

pp. e0202702 ◽

Cited By ~ 5

Author(s):

Lutz Claes ◽

Nicholaus Meyers ◽

Julian Schülke ◽

Sandra Reitmaier ◽

Svenja Klose ◽

...

Keyword(s):

Bone Formation ◽

Callus Distraction ◽

Interfragmentary Movement

Download Full-text

A Novel Cell-Loading Method into Porous β-TCP Blocks

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.993 ◽

2006 ◽

Vol 309-311 ◽

pp. 993-996

Author(s):

Ichiro Torigoe ◽

Shinichi Sotome ◽

A. Tsuchiya ◽

Kenichi Shinomiya

Keyword(s):

Bone Formation ◽

The Novel ◽

Cell Loading ◽

Pressure Method ◽

Loading Method ◽

Loading Efficiency ◽

Novel Method ◽

Loading Methods ◽

In Vivo Bone Formation

In the field of bone tissue engineering using cells combined with scaffolds, it is important to efficiently load cells into porous scaffolds. We devised a novel cell-loading method into porous beta-tricalcium phosphate (β-TCP) blocks. In this study, we compared this method with two conventional cell-loading methods in terms of cell-loading efficiency and in vivo bone formation capability. Bone marrow stromal cells (BMSCs) were obtained from the femurs of Fisher rats. After about 10 days of culture, BMSCs were harvested and suspended in the plasma of the Fisher rats at a concentration of 2×106 cells/ml. This cell suspension was loaded into porous β-TCP cubes (5×5×5mm) by using three loading methods: a soaking method, a post low-pressure method and a pre low-pressure method (the novel method). These β-TCP cubes were cross-sectioned and stained with toluidine blue and cell-counted. Cell-loading efficiency was significantly higher when using the novel methods. For the study of in vivo bone formation capability, nearly confluent BMSCs were exposed in an osteogenic medium supplemented with 10-7 M dexamethasone, 50µg/ml L-ascorbic acid phosphate and 10mM β-glycerophosphate for 4 days. These osteogenic cells were harvested and suspended in the plasma of the Fisher rats at a concentration of 2×106 cells/ml. This cell suspension was loaded into porous β-TCP cubes (5×5×5mm) by using the three cell-loading methods. Immediately, these β-TCP cubes were implanted at subcutaneous sites in the backs of 7-week-old male Fisher rats and harvested at postoperative 3 and 6 weeks. After cross-sectioning, these sections were stained with hematoxylin and eosin, and the new bone formation area was quantified. Consistent with cell-loading efficiency, in vivo bone formation capability was significantly higher in the novel method at postoperative 6 weeks. We showed the usefulness of the novel cell-loading method in bone tissue engineering.

Download Full-text

The removal and morphology of intact biofilms from implanted venous access devices

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141470 ◽

1995 ◽

Vol 53 ◽

pp. 1020-1021

Author(s):

M.A. Gregory ◽

G.P. Hadley

Keyword(s):

Patient Selection ◽

Surgical Oncology ◽

Venous Access ◽

Common Procedure ◽

Indwelling Catheters ◽

Careful Patient ◽

Novel Method ◽

The Subject ◽

Vascular Catheters ◽

Venous Access Devices

The insertion of implanted venous access systems for children undergoing prolonged courses of chemotherapy has become a common procedure in pediatric surgical oncology. While not permanently implanted, the devices are expected to remain functional until cure of the primary disease is assured. Despite careful patient selection and standardised insertion and access techniques, some devices fail. The most commonly encountered problems are colonisation of the device with bacteria and catheter occlusion. Both of these difficulties relate to the development of a biofilm within the port and catheter. The morphology and evolution of biofilms in indwelling vascular catheters is the subject of ongoing investigation. To date, however, such investigations have been confined to the examination of fragments of biofilm scraped or sonicated from sections of catheter. This report describes a novel method for the extraction of intact biofilms from indwelling catheters.15 children with Wilm’s tumour and who had received venous implants were studied. Catheters were removed because of infection (n=6) or electively at the end of chemotherapy.

Download Full-text