An evidence of pores in phospholipid membrane induced by an antimicrobial peptide NK-2

2022 ◽  
pp. 106759
Author(s):  
Animesh Halder ◽  
Sanat Karmakar
Keyword(s):  
Antimicrobial Peptide ◽  
Phospholipid Membrane

scholarly journals Dynamical and Phase Behavior of a Phospholipid Membrane Altered by an Antimicrobial Peptide at Low Concentration

2016 ◽  
Vol 7 (13) ◽  
pp. 2394-2401 ◽  
Author(s):  
V. K. Sharma ◽  
E. Mamontov ◽  
M. Tyagi ◽  
S. Qian ◽  
D. K. Rai ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Phase Behavior ◽  
Phospholipid Membrane ◽  
Low Concentration

scholarly journals Effects of Antibacterial Peptide F1 on Bacterial Liposome Membrane Integrity

2021 ◽  
Vol 8 ◽  
Author(s):  
Qun Wang ◽  
Bo Peng ◽  
Mingyue Song ◽  
Abdullah ◽  
Jun Li ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Membrane Structure ◽  
Bacterial Membrane ◽  
Phospholipid Membranes ◽  
Phospholipid Membrane ◽  
Gram Positive ◽  
Liposome Membrane ◽  
Gram Negative ◽  
Bacterial Membranes ◽  
Gram Positive Bacteria

Previous studies from our lab have shown that the antimicrobial peptide F1 obtained from the milk fermentation by Lactobacillus paracasei FX-6 derived from Tibetan kefir was different from common antimicrobial peptides; specifically, F1 simultaneously inhibited the growth of Gram-negative and Gram-positive bacteria. Here, we present follow-on work demonstrating that after the antimicrobial peptide F1 acts on either Escherichia coli ATCC 25922 (E. coli) or Staphylococcus aureus ATCC 63589 (S. aureus), their respective bacterial membranes were severely deformed. This deformation allowed leakage of potassium and magnesium ions from the bacterial membrane. The interaction between the antimicrobial peptide F1 and the bacterial membrane was further explored by artificially simulating the bacterial phospholipid membranes and then extracting them. The study results indicated that after the antimicrobial peptide F1 interacted with the bacterial membranes caused significant calcein leakage that had been simulated by different liposomes. Furthermore, transmission electron microscopy observations revealed that the phospholipid membrane structure was destroyed and the liposomes presented aggregation and precipitation. Quartz Crystal Microbalance with Dissipation (QCM-D) results showed that the antimicrobial peptide F1 significantly reduced the quality of liposome membrane and increased their viscoelasticity. Based on the study's findings, the phospholipid membrane particle size was significantly increased, indicating that the antimicrobial peptide F1 had a direct effect on the phospholipid membrane. Conclusively, the antimicrobial peptide F1 destroyed the membrane structure of both Gram-negative and Gram-positive bacteria by destroying the shared components of their respective phospholipid membranes which resulted in leakage of cell contents and subsequently cell death.

scholarly journals Antimicrobial Peptide Impacts the Lateral Diffusion and Bending Rigidity of Phospholipid Membrane

2016 ◽  
Vol 110 (3) ◽  
pp. 246a ◽  
Author(s):  
Veerendra K. Sharma ◽  
E. Mamontov ◽  
D.B. Anunciado ◽  
M. Ohl ◽  
H. O’Neill ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Lateral Diffusion ◽  
Phospholipid Membrane ◽  
Bending Rigidity

The Human Endogenous Antimicrobial Peptide Ll-37 Stimulates Airway Epithelial Repair

Pneumologie ◽  
2006 ◽  
Vol 59 (12) ◽  
Author(s):  
R Shaykhiev ◽  
C Beißwenger ◽  
K Kändler ◽  
J Senske ◽  
A Püchner ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Airway Epithelial ◽  
Epithelial Repair

Antimicrobial Peptide Prodrugs and Mimetics

2016 ◽  
Author(s):  
Marc Devocelle ◽  
Éanna Forde ◽  
André Schütte ◽  
Andrea Molero-Bondia ◽  
Emer Reeves ◽  
...  
Keyword(s):  
Antimicrobial Peptide

LL-37; The Human Epithelial Antimicrobial Peptide and Innate Immunity System

2015 ◽  
Vol 1 (4) ◽  
pp. 76
Author(s):  
Seyadeh Zahra Sajjadiyan ◽  
Sarah Mohammadinejad ◽  
Leila Hassani
Keyword(s):  
Innate Immunity ◽  
Antimicrobial Peptide
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