scholarly journals Dynamical Behavior of the CAT Domain from Cre Recombinase, in the Absence of and with the CIS Interaction of the N Helix

2021 ◽  
Vol 120 (3) ◽  
pp. 17a
Author(s):  
Marco A. Ramírez
Keyword(s):  
Dynamical Behavior ◽  
Cre Recombinase ◽  
Cis Interaction

Dynamical behavior of a nonlinear rotorcraft model

1989 ◽  
Author(s):  
GEORGE FLOWERS ◽  
BENSONH. TONGUE
Keyword(s):  
Dynamical Behavior

scholarly journals Radiation-induced toxicity in rectal epithelial stem cell contributes to acute radiation injury in rectum

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Felipe Rodriguez Tirado ◽  
Payel Bhanja ◽  
Eduardo Castro-Nallar ◽  
Ximena Diaz Olea ◽  
Catalina Salamanca ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Cre Recombinase ◽  
Lineage Tracing ◽  
Common Side Effect ◽  
Rectal Injury ◽  
Male Mice ◽  
Pelvic Irradiation ◽  
Pelvic Malignancies ◽  
Radiation Induced

Abstract Background Radiation-induced rectal epithelial damage is a very common side effect of pelvic radiotherapy and often compromise the life quality and treatment outcome in patients with pelvic malignancies. Unlike small bowel and colon, effect of radiation in rectal stem cells has not been explored extensively. Here we demonstrate that Lgr5-positive rectal stem cells are radiosensitive and organoid-based transplantation of rectal stem cells mitigates radiation damage in rectum. Methods C57Bl6 male mice (JAX) at 24 h were exposed to pelvic irradiation (PIR) to determine the radiation effect in pelvic epithelium. Effect of PIR on Lgr5-positive rectal stem cells (RSCs) was determined in Lgr5-EGFP-Cre-ERT2 mice exposed to PIR. Effect of PIR or clinically relevant fractionated PIR on regenerative response of Lgr5-positive RSCs was examined by lineage tracing assay using Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato mice with tamoxifen administration to activate Cre recombinase and thereby marking the ISC and their respective progeny. Ex vivo three-dimensional organoid cultures were developed from Lgr5-EGFP-Cre-ERT2 mice. Organoid growth was determined by quantifying the budding crypt/total crypt ratio. Organoids from Lgr5-EGFP-ires-CreERT2-TdT mice were transplanted in C57Bl6 male mice exposed to PIR. Engraftment and repopulation of Lgr5-positive RSCs were determined after tamoxifen administration to activate Cre recombinase in recipient mice. Statistical analysis was performed using Log-rank (Mantel-Cox) test and paired two-tail t test. Result Exposure to pelvic irradiation significantly damaged rectal epithelium with the loss of Lgr5+ve rectal stem cells. Radiosensitivity of rectal epithelium was also observed with exposure to clinically relevant fractionated pelvic irradiation. Regenerative capacity of Lgr5+ve rectal stem cells was compromised in response to fractionated pelvic irradiation. Ex vivo organoid study demonstrated that Lgr5+ve rectal stem cells are sensitive to both single and fractionated radiation. Organoid-based transplantation of Lgr5+ve rectal stem cells promotes repair and regeneration of rectal epithelium. Conclusion Lgr5-positive rectal stem cells are radiosensitive and contribute to radiation-induced rectal epithelial toxicity. Transplantation of Lgr5-positive rectal stem cells mitigates radiation-induced rectal injury and promotes repair and regeneration process in rectum.

Probing the dynamical behavior in glass transition of PVPh-PEO blend

2021 ◽  
Vol 554 ◽  
pp. 120561
Author(s):  
Yong-jin Peng ◽  
He-Huang ◽  
Chang-jun Wang ◽  
Zhong-fu Zuo ◽  
Xue-zheng Liu
Keyword(s):  
Glass Transition ◽  
Dynamical Behavior

scholarly journals A Characterization of the Dynamics of Schröder’s Method for Polynomials with Two Roots

2021 ◽  
Vol 5 (1) ◽  
pp. 25
Author(s):  
Víctor Galilea ◽  
José M. Gutiérrez
Keyword(s):  
Nonlinear Equations ◽  
Complex Plane ◽  
Iterative Process ◽  
Dynamical Behavior ◽  
Basins Of Attraction ◽  
Schröder’S Method

The purpose of this work is to give a first approach to the dynamical behavior of Schröder’s method, a well-known iterative process for solving nonlinear equations. In this context, we consider equations defined in the complex plane. By using topological conjugations, we characterize the basins of attraction of Schröder’s method applied to polynomials with two roots and different multiplicities. Actually, we show that these basins are half-planes or circles, depending on the multiplicities of the roots. We conclude our study with a graphical gallery that allow us to compare the basins of attraction of Newton’s and Schröder’s method applied to some given polynomials.

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