Density-dependent enhancement of methane oxidation activity and growth of Methylocystis sp. by a non-methanotrophic bacterium Sphingopyxis sp

Abstract. While the importance of anaerobic methane oxidation has been reported for marine ecosystems, the role of this process in soils is still questionable. Grasslands used as pastures for cattle overwintering show an increase in anaerobic soil micro-sites caused by animal treading and excrement deposition. Therefore, anaerobic potential methane oxidation activity of severely impacted soil from a cattle winter pasture was investigated in an incubation experiment under anaerobic conditions using 13C-labelled methane. We were able to detect a high microbial activity utilizing CH4 as nutrient source shown by the respiration of 13CO2. Measurements of possible terminal electron acceptors for anaerobic oxidation of methane were carried out. Soil sulfate concentrations were too low to explain the oxidation of the amount of methane added, but enough nitrate and iron(III) were detected. However, only nitrate was consumed during the experiment. 13C-PLFA analyses clearly showed the utilization of CH4 as nutrient source mainly by organisms harbouring 16:1ω7 PLFAs. These lipids were also found as most 13C-enriched fatty acids by Raghoebarsing et al. (2006) after addition of 13CH4 to an enrichment culture coupling denitrification of nitrate to anaerobic oxidation of methane. This might be an indication for anaerobic oxidation of methane by relatives of "Candidatus Methylomirabilis oxyfera" in the investigated grassland soil under the conditions of the incubation experiment.

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On the methane oxidation activity of Sr2(MgMo)2O6-δ: a potential anode material for direct hydrocarbon solid oxide fuel cells

Journal of Materials Chemistry ◽

10.1039/c1jm10523j ◽

2011 ◽

Vol 21 (20) ◽

pp. 7443 ◽

Cited By ~ 15

Author(s):

Michael van den Bossche ◽

Steven McIntosh

Keyword(s):

Fuel Cells ◽

Solid Oxide Fuel Cells ◽

Anode Material ◽

Methane Oxidation ◽

Solid Oxide ◽

Oxide Fuel ◽

Oxidation Activity

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Abundance and Activity of Methanotrophic Bacteria in Littoral and Profundal Sediments of Lake Constance (Germany)

Applied and Environmental Microbiology ◽

10.1128/aem.01350-08 ◽

2008 ◽

Vol 75 (1) ◽

pp. 119-126 ◽

Cited By ~ 60

Author(s):

M. Rahalkar ◽

J. Deutzmann ◽

B. Schink ◽

I. Bussmann

Keyword(s):

Methane Oxidation ◽

Lake Constance ◽

Type I ◽

Sediment Depth ◽

Oxidation Activity ◽

Oxidation Rates ◽

Methane Oxidizing Bacteria ◽

Littoral Sediment ◽

Sediment Layers

ABSTRACT The abundances and activities of aerobic methane-oxidizing bacteria (MOB) were compared in depth profiles of littoral and profundal sediments of Lake Constance, Germany. Abundances were determined by quantitative PCR (qPCR) targeting the pmoA gene and by fluorescence in situ hybridization (FISH), and data were compared to methane oxidation rates calculated from high-resolution concentration profiles. qPCR using type I MOB-specific pmoA primers indicated that type I MOB represented a major proportion in both sediments at all depths. FISH indicated that in both sediments, type I MOB outnumbered type II MOB at least fourfold. Results obtained with both techniques indicated that in the littoral sediment, the highest numbers of methanotrophs were found at a depth of 2 to 3 cm, corresponding to the zone of highest methane oxidation activity, although no oxygen could be detected in this zone. In the profundal sediment, highest methane oxidation activities were found at a depth of 1 to 2 cm, while MOB abundance decreased gradually with sediment depth. In both sediments, MOB were also present at high numbers in deeper sediment layers where no methane oxidation activity could be observed.

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Methanotrophic activity and diversity in different <i>Sphagnum magellanicum</i> dominated habitats in the southernmost peat bogs of Patagonia

Biogeosciences ◽

10.5194/bg-9-47-2012 ◽

2012 ◽

Vol 9 (1) ◽

pp. 47-55 ◽

Cited By ~ 42

Author(s):

N. Kip ◽

C. Fritz ◽

E. S. Langelaan ◽

Y. Pan ◽

L. Bodrossy ◽

...

Keyword(s):

Methane Oxidation ◽

Northern Hemisphere ◽

Water Levels ◽

Peat Bogs ◽

Oxidation Activity ◽

Moss Species ◽

Sphagnum Mosses ◽

Methane Concentrations ◽

Sphagnum Magellanicum

Abstract. Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH4 gDW−1 day−1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH4 l−1 pore water). Little methanotrophic activity (<0.5 μmol CH4 gDW−1 day−1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH4 gDW−1 day−1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation.

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Methane oxidation activity and diversity of aerobic methanotrophs in pH-neutral and semi-neutral thermal springs of the Kunashir Island, Russian Far East

Extremophiles ◽

10.1007/s00792-013-0603-z ◽

2013 ◽

Vol 18 (2) ◽

pp. 207-218 ◽

Cited By ~ 10

Author(s):

A. K. Kizilova ◽

M. V. Sukhacheva ◽

N. V. Pimenov ◽

A. M. Yurkov ◽

I. K. Kravchenko

Keyword(s):

Methane Oxidation ◽

Russian Far East ◽

Far East ◽

Thermal Springs ◽

Oxidation Activity ◽

Kunashir Island ◽

Aerobic Methanotrophs

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Pd (1 wt%)/LaMn0.4Fe0.6O3 Catalysts Supported Over Silica SBA-15: Effect of Perovskite Loading and Support Morphology on Methane Oxidation Activity and SO2 Tolerance

Topics in Catalysis ◽

10.1007/s11244-012-9867-2 ◽

2012 ◽

Vol 55 (11-13) ◽

pp. 782-791 ◽

Cited By ~ 8

Author(s):

L. F. Liotta ◽

G. Di Carlo ◽

G. Pantaleo ◽

J. C. Hernandez Garrido ◽

A. M. Venezia

Keyword(s):

Methane Oxidation ◽

Oxidation Activity ◽

So2 Tolerance

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Close-Stacking of Iron-Oxo-Based Double-Decker Complex on Graphite Surface Achieved High Catalytic CH4 Oxidation Activity Comparable to that of Methane Monooxygenases

10.26434/chemrxiv.14728860.v1 ◽

2021 ◽

Author(s):

Yasuyuki Yamada ◽

Kentaro Morita ◽

Takuya Sugiura ◽

Yuka Toyoda ◽

Nozomi Mihara ◽

...

Keyword(s):

Aqueous Solution ◽

Methane Oxidation ◽

Graphite Surface ◽

Iron Phthalocyanine ◽

Ch4 Oxidation ◽

Oxidation Activity ◽

Phthalocyanine Complex ◽

Double Decker ◽

Dinuclear Iron

<div><div><div><p>Herein, we report that the close-stacking of a double-decker-type dinuclear iron phthalocyanine complex on a graphite surface is effective for achieving high methane oxidation activity, comparable to those of certain MMOs, in an aqueous solution. </p></div></div></div>

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Anaerobic oxidation of methane in grassland soils used for cattle husbandry

Biogeosciences Discussions ◽

10.5194/bgd-9-4919-2012 ◽

2012 ◽

Vol 9 (4) ◽

pp. 4919-4945

Author(s):

A. Bannert ◽

C. Bogen ◽

J. Esperschütz ◽

A. Koubová ◽

F. Buegger ◽

...

Keyword(s):

Methane Oxidation ◽

Anaerobic Methane Oxidation ◽

Anaerobic Oxidation Of Methane ◽

Type I ◽

Anaerobic Oxidation ◽

Nutrient Source ◽

Oxidation Activity ◽

Oxidation Of Methane ◽

Cattle Husbandry

Abstract. While the importance of anaerobic methane oxidation has been reported for marine ecosystems, the role of this process in soils is still questionable. Grasslands used as pastures for cattle-overwintering show an increase in anaerobic soil micro-sites caused by animal treading and excrement deposition. Therefore anaerobic potential methane oxidation activity of severely impacted soil from a cattle winter pasture was investigated in an incubation experiment under anaerobic conditions using 13C-labeled methane. We were able to detect a high microbial activity utilizing CH4 as nutrient source shown by the respiration of 13CO2. Measurements of possible terminal electron acceptors for anaerobic oxidation of methane were carried out. Soil sulfate concentrations were too low to explain the oxidation of the amount of methane added, but enough nitrate and iron(III) were detected. However, only nitrate was consumed during the experiment. 13C-PLFA analyses clearly showed the utilization of CH4 as nutrient source mainly by organisms harbouring 16:1ω7 PLFAs. These lipids were found in Gram-negative microorganisms and anaerobes. The fact that these lipids are also typical for type I methanotrophs, known as aerobic methane oxidizers, might indicate a link between aerobic and anaerobic methane oxidation.

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