Genotoxic potential of Polycyclic Aromatic Hydrocarbons-coated onto airborne Particulate Matter (PM2.5) in human lung epithelial A549 cells

2008 ◽  
Vol 270 (1) ◽  
pp. 144-155 ◽  
Author(s):  
Sylvain Billet ◽  
Imane Abbas ◽  
Jérémie Le Goff ◽  
Anthony Verdin ◽  
Véronique André ◽  
...  
Author(s):  
Aniela Matuszewska ◽  
Maria Czaja

The applicability of the fluorescence techniques to identify the polycyclic aromatic hydrocarbons (PAHs) in environmental samples is presented. The technique of synchronous fluorescence enabled the identification of the PAHs series containing 2–6 condensed rings in urban airborne particulate matter from Upper Silesia industrial region in Poland. The results obtained by synchronous and conventional fluorescence measurements have been confirmed by those from gas chromatography-mass spectrometry. As the air sample was taken in summer season, the main source of pollution by PAHs component seems to be transport – the exhaust gases from motor vehicles.


2010 ◽  
Vol 93 (1) ◽  
pp. 284-294 ◽  
Author(s):  
Antonia Garrido Frenich ◽  
Rosa Martínez Ocaña ◽  
José L Martínez Vidal

Abstract An accurate and sensitive method for the identification and the quantification of 24 polycyclic aromatic hydrocarbons (PAHs) in airborne particulate matter has been developed and validated using GC/MS/MS with a triple quadrupole analyzer. Ultrasonic extraction (USE) and pressurized liquid extraction (PLE) were evaluated. PLE was selected because it provided better recoveries and reduced treatment time and use of solvents compared to USE. Cleanup of PLE extracts was not necessary. The accuracy of the optimized method was tested using a standard reference material of urban dust (SRM 1649a). PAH recoveries were between 70 and 99 (except for naphthalene, which was lower than 20) with RSDs ranging from 2.6 to 15.3. Linearity in the range 5500 ng/mL provided R2 values higher than 0.99 for all compounds. LODs for PAHs ranged from 0.001 to 0.435 ng/mL and LOQs ranged from 0.003 to 0.757 ng/mL. The proposed method was applied to the analysis of real samples collected in the city of Almera, Spain, where some PAHs were found at levels lower than the legislated limit.


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