Optimization of fermentation conditions for the production of pullulan by a new strain of Aureobasidium pullulans isolated from sea mud and its characterization

2012 ◽  
Vol 87 (2) ◽  
pp. 1696-1700 ◽  
Author(s):  
Shengjun Wu ◽  
Jing Chen ◽  
Saikun Pan
2014 ◽  
Vol 692 ◽  
pp. 167-171
Author(s):  
Ming Jing Sun ◽  
Li Qing Sun ◽  
Shan Shan Wang ◽  
Kai Zhao

The components of a fermentation medium were optimized to produce cellulose from HDZK-BYTF620 (Aureobasidium pullulans) which is from the gut of termites and can produce cellulose under liquid state fermentation conditions by response surface methodology. By Plackett-Burman design screened out three major factors of strains HDZK-BYTF620 which affected production of the activity cellulose, immediately, CaCl2, MgSO4and CMC-Na. The fermentation conditions were optimized with Box-Behnken experiment and the largest regional of the activity of cellulase was approach with the steepest ascent experiment. The optimum composition of medium addition of CaCl2, MgSO4and CMC-Na was 0.77 g/L, 0.41 g/L and 21.88 g/L, respectively. Under the optimum fermentation medium conditions, the activity of cellulase was (598.00±2.56)×10-3IU/mL, increased 5.0 % to that of using initial medium.


2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Duong Long Duy ◽  
Pham Minh Vu ◽  
Nguyen Tri Nhan ◽  
Tran Linh Thuoc ◽  
Dang Thi Phuong Thao

2014 ◽  
Vol 1073-1076 ◽  
pp. 286-291
Author(s):  
Yong Zuo ◽  
Peng Jiang ◽  
Xiao Long Wang ◽  
Bin Fu ◽  
Xiao Long Yang ◽  
...  

The fermentation conditions of Bacterial Strain T2013 were studied. The fermentation conditions of T2013 were investigated with single-factor experiment. In addition to, the fermentation conditions were optimized through the orthogonal test. The results showed the highest production of blue pigment was obtained on condition that fermentation time was 2d, the liquid volume was 50ml in the 250ml shake-flask, the inoculum volume was 2%, the initial value of pH was 7.6, fermentation temperature was 37°C and the speed was 150r/min.The fermentation conditions of Bacterial Strain T2013 were preliminarily determined,whichprovides a theoretical basis for the industrial production of blue pigment.


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