Optimization of Fermentation Conditions for Cellulase Production by HDZK-BYTF620 (Aureobasidium pullulans) which is from the Gut of Termites

2014 ◽  
Vol 692 ◽  
pp. 167-171
Author(s):  
Ming Jing Sun ◽  
Li Qing Sun ◽  
Shan Shan Wang ◽  
Kai Zhao
Keyword(s):  
Aureobasidium Pullulans ◽  
Fermentation Medium ◽  
Cellulase Production ◽  
Fermentation Conditions ◽  
Steepest Ascent ◽  
Burman Design ◽  
Major Factors ◽  
Initial Medium ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

The components of a fermentation medium were optimized to produce cellulose from HDZK-BYTF620 (Aureobasidium pullulans) which is from the gut of termites and can produce cellulose under liquid state fermentation conditions by response surface methodology. By Plackett-Burman design screened out three major factors of strains HDZK-BYTF620 which affected production of the activity cellulose, immediately, CaCl2, MgSO4and CMC-Na. The fermentation conditions were optimized with Box-Behnken experiment and the largest regional of the activity of cellulase was approach with the steepest ascent experiment. The optimum composition of medium addition of CaCl2, MgSO4and CMC-Na was 0.77 g/L, 0.41 g/L and 21.88 g/L, respectively. Under the optimum fermentation medium conditions, the activity of cellulase was (598.00±2.56)×10-3IU/mL, increased 5.0 % to that of using initial medium.

Fermentation Conditions Optimization of Anti-Tumor Active Metabolites from Marine Penicillium sp. HGQ6

2011 ◽  
Vol 340 ◽  
pp. 344-350
Author(s):  
Guo Lei ◽  
Wen Cheng Zhu ◽  
Chao Liu
Keyword(s):  
Optimal Conditions ◽  
Inhibition Activity ◽  
Active Metabolites ◽  
Fermentation Conditions ◽  
Burman Design ◽  
Predicted Values ◽  
Initial Medium ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design ◽  
Active Substances

The objectives of this study were to optimize fermentation conditons of anti-tumor active metabolites from marine penicillium sp. HGQ6. Based on the inhibition activity against stomach cancer cell BGC823, firstly, the initial medium was screened, then seven factors including peptone, sucrose, K2HPO4, FeSO4, pH, temperature and agitation were investigated by Plackett-Burman design, and the important variables including pH, temperature and K2HPO4 were optimized by using Response surface methodology (RSM). Experimental results showed that the optimum fermentation conditions were peptone 1%, sucrose 3%, K2HPO4 0.06%, FeSO4 0.001%, pH6.41, temperature 35°C and agitation 180 r·min-1. Under these optimal conditions, the actual inhibition ratio achieved 90.4%, which agreed with the predicted values. This indicated the good feasibility of Plackett-Burman design and RSM in the optimization of fermentation conditions for production of anti-tumor active substances from stain HGQ6.

Enhanced Production of Mycelia by the Medicinal Mushroom Cordyceps militaris Using Plackett-Burman Design and Response Surface Methodology

2011 ◽  
Vol 138-139 ◽  
pp. 1209-1214
Author(s):  
Xiao Yu Liu ◽  
Fan Xing Meng ◽  
Yi Bo Zhang ◽  
Huan He ◽  
Wei Han ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Cordyceps Militaris ◽  
Enhanced Production ◽  
Burman Design ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

Response surface methodology (RSM) was used for statistical optimization of fermentation medium that influenced the yield of endo-polysaccharide from cultivated mycelia of Cordyceps militaris. First, the Plackett-Burman design was used to evaluate the effects of ten variables including glucose, maltose, peptone, yeast extract, KH2PO4, MgSO4, CaCl2, VB1, inoculum density and medium capacity. Among these variables, glucose, peptone and yeast extract were identified to have the significant effects. Subsequently, response surface methodology based on a five-level three-factor central composite design was employed to determine the maximum dry weight (DW) of mycelial biomass at optimum concentration of glucose, peptone and yeast extract. The mycelia growth was found to correlate to the three parameters that could be represented by second-order polynomial models. The optimal values of the three parameters were determined as 4.62% glucose, 3.36% peptone and 0.43% yeast extract. The prediction DW was 23.727g/L. The actual experimental results were in agreement with the prediction.

scholarly journals Improved Production of Sublancin 168 Biosynthesized byBacillus subtilis168 Using Chemometric Methodology and Statistical Experimental Designs

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Shengyue Ji ◽  
Weili Li ◽  
Haiyun Xin ◽  
Shan Wang ◽  
Binyun Cao
Keyword(s):  
Soybean Meal ◽  
Incubation Temperature ◽  
Optimal Combination ◽  
Fermentation Condition ◽  
Box Behnken Design ◽  
Steepest Ascent ◽  
Burman Design ◽  
Fermentation Parameters ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

Sublancin 168, as a distinct S-linked antimicrobial glycopeptide produced byBacillus subtilis168, is effective in killing specific microorganisms. However, the reported yield of sublancin 168 is at a low level of no more than 60 mg from 1 L fermentation culture ofB. subtilis168 by using the method in the literature. Thus optimization of fermentation condition for efficiently producing sublancin 168 is required. Here, Box-Behnken design was used to determine the optimal combination of three fermentation parameters, namely, corn powder, soybean meal, and temperature that were identified previously by Plackett-Burman design and the steepest ascent experiment. Subsequently, based on the response surface methodology, the quadratic regression model for optimally producing sublancin 168 was developed, and the optimal combination of culture parameters for maximum sublancin 168 production of 129.72 mg/L was determined as corn powder 28.49 g/L, soybean meal 22.99 g/L, and incubation temperature 30.8°C. The results showed that sublancin 168 production obtained experimentally was coincident with predicted value of 125.88 mg/L, and the developed model was proved to be adequate, and the aim of efficiently producing sublancin 168 was achieved.

Medium Optimization for the Antibacterial Substances Production from Brevibacillus brevis XDH Using Response Surface Methodology

2011 ◽  
Vol 345 ◽  
pp. 355-360 ◽  
Author(s):  
Zhen Song ◽  
Xun Li Liu ◽  
Chang Xu Lu ◽  
Jian Yu ◽  
Rui Cheng Ju ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Antibacterial Activity ◽  
Response Surface ◽  
Medium Optimization ◽  
Fermentation Medium ◽  
Steepest Ascent ◽  
High Antibacterial Activity ◽  
Burman Design ◽  
Brevibacillus Brevis ◽  
Plackett Burman Design

Brevibacillus Brevis XDH is an antagonistic bacterium with high antibacterial activity against many pathogens. Fermentation medium for the production of the antibacterial substances was statistically optimized by Plackett-Burman design, steepest ascent experiments and response surface methodology. The optimized medium was composed of (g/L): glucose 15.6, soybean meal 28.4, starch 6, CaCO3 2 and MgSO4 4. After optimization, the production of antibacterial metabolites increased more than 70% than that of control.

scholarly journals Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Li-mei Lin ◽  
Ling-jia Zhao ◽  
Jing Deng ◽  
Su-hui Xiong ◽  
Jie Tang ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Steepest Ascent ◽  
Penthorum Chinense Pursh ◽  
Ft Ir ◽  
Experimental Yield ◽  
Burman Design ◽  
Anti Inflammatory ◽  
Penthorum Chinense ◽  
Optimized Conditions ◽  
Plackett Burman Design

Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

Sign in / Sign up

Export Citation Format

Share Document