scholarly journals Structural investigation of water-soluble polysaccharides extracted from the fruit bodies of Coprinus comatus

2013 ◽  
Vol 91 (1) ◽  
pp. 314-321 ◽  
Author(s):  
Bo Li ◽  
Justyna M. Dobruchowska ◽  
Gerrit J. Gerwig ◽  
Lubbert Dijkhuizen ◽  
Johannis P. Kamerling
Keyword(s):  
Structural Investigation ◽  
Water Soluble ◽  
Coprinus Comatus

Structural investigation and comparative cytotoxic activity of water-soluble polysaccharides from fruit bodies of the medicinal fungus quinine conk

2020 ◽  
Vol 175 ◽  
pp. 112313
Author(s):  
Victoria V. Golovchenko ◽  
Shinen Naranmandakh ◽  
Jamsranjav Ganbaatar ◽  
Artur Yu. Prilepskii ◽  
Gennady L. Burygin ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Structural Investigation ◽  
Water Soluble ◽  
Medicinal Fungus

Structural investigation of water-hyacinth (Eichhornia crassipes) polysaccharides. Part I. Water-soluble polysaccharides

1988 ◽  
Vol 66 (11) ◽  
pp. 2777-2781 ◽  
Author(s):  
Mohamady A. Issa
Keyword(s):  
Water Hyacinth ◽  
Eichhornia Crassipes ◽  
Structural Investigation ◽  
Main Chain ◽  
Cold Water ◽  
Water Extract ◽  
Water Soluble ◽  
Hot Water ◽  
Side Chains ◽  
Hot Water Extract

A galactomannan and a branched (1 → 3)-β-D-glucan were isolated from the water hyacinth plant. The galactomannan, purified from the cold water extract, is composed of D-galactose and D-mannose in a ratio of 1.0:2.8. It has a (1 → 4)-linked D-mannose backbone, one out of three D-mannose residues being substituted with a single α-D-galactosyl unit. The branched (1 → 3)-β-D-glucan isolated from the hot water extract has a main chain composed of β-(1 → 3)-linked D-glucopyranosyl residues, and two single β(1 → 6)-D-glucopyranosyl groups attached as side chains to, on average, every 5 sugar units of the main chain. In addition, the branching of the β-glucan occurs regularly at O-6 of the β-(1 → 3)-linked backbone.

Purification and structural investigation of a water-soluble polysaccharide from Flammulina velutipes

2012 ◽  
Vol 87 (3) ◽  
pp. 2279-2283 ◽  
Author(s):  
An-qiang Zhang ◽  
Nan-nan Xiao ◽  
Ying-lin Deng ◽  
Peng-fei He ◽  
Pei-long Sun
Keyword(s):  
Structural Investigation ◽  
Water Soluble ◽  
Flammulina Velutipes ◽  
Soluble Polysaccharide

Structural investigation of a water-soluble glucan from an edible mushroom, Astraeus hygrometricus

2004 ◽  
Vol 339 (13) ◽  
pp. 2249-2254 ◽  
Author(s):  
Indranil Chakraborty ◽  
Soumitra Mondal ◽  
Malay Pramanik ◽  
Dilip Rout ◽  
Syed S. Islam
Keyword(s):  
Structural Investigation ◽  
Edible Mushroom ◽  
Water Soluble

A Resorcinol-Formaldehyde Resin as an Embedding Material for Electron Microscopy of Membranes

1969 ◽  
Vol 27 ◽  
pp. 328-329 ◽  
Author(s):  
J. G. Robertson ◽  
D. F. Parsons
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Neutral Lipids ◽  
Lipid Extraction ◽  
Water Soluble ◽  
Formaldehyde Resin ◽  
Electron Microscope Autoradiography ◽  
Resorcinol Formaldehyde ◽  
Major Disadvantage ◽  
Cell Organization

The extraction of lipids from tissues during fixation and embedding for electron microscopy is widely recognized as a source of possible artifact, especially at the membrane level of cell organization. Lipid extraction is also a major disadvantage in electron microscope autoradiography of radioactive lipids, as in studies of the uptake of radioactive fatty acids by intestinal slices. Retention of lipids by fixation with osmium tetroxide is generally limited to glycolipids, phospholipids and highly unsaturated neutral lipids. Saturated neutral lipids and sterols tend to be easily extracted by organic dehydrating reagents prior to embedding. Retention of the more saturated lipids in embedded tissue might be achieved by developing new cross-linking reagents, by the use of highly water soluble embedding materials or by working at very low temperatures.

Ferritin Labeled Antibody Staining of Thin Sections

1969 ◽  
Vol 27 ◽  
pp. 420-421
Author(s):  
J. D. McLean ◽  
S. J. Singer
Keyword(s):  
Biological Material ◽  
Water Soluble ◽  
Thin Sections ◽  
Antibody Staining ◽  
Thin Sectioning ◽  
Ultrathin Sections

The successful application of ferritin labeled antibodies (F-A) to ultrathin sections of biological material has been hampered by two main difficulties. Firstly the normally used procedures for the preparation of material for thin sectioning often result in a loss of antigenicity. Secondly the polymers employed for embedding may non-specifically absorb the F-A. Our earlier use of cross-linked polyampholytes as embedding media partially overcame these problems. However the water-soluble monomers used for this method still extract many lipids from the material.

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