Lipid Inclusions in Cardiac Myocytes - A Rare Case of Cardiolipotoxicity

The heart oxidizes fatty acids for its energy production. The physiological balance between fatty acid uptake and its oxidation prevents lipid accumulation in cardiac myocytes. However, accumulation of lipids due to various processes such as obesity, diabetes, heart failure, myocardial ischemia or infarction can result in damage to the heart tissue, also known as cardiolipotoxicity. We present a unique case of a 69-year-old gentleman with a history of heart failure and ventricular tachycardia. Endomyocardial biopsy to assess for restrictive cardiomyopathy/amyloid showed no evidence of amyloid, significant inflammation or fibrosis, but did show intracellular accumulation of significant amorphous material in most cardiac myocytes. We review the literature regarding the pathogenesis of cardiolipotoxicity, which has no definite cause or treatment yet identified.

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Hamartoma of mature cardiac myocytes: Report of a rare case with review of literature

Journal of the Practice of Cardiovascular Sciences ◽

10.4103/jpcs.jpcs_43_18 ◽

2018 ◽

Vol 4 (3) ◽

pp. 254

Author(s):

MahmoodDhahir Al-Mendalawi

Keyword(s):

Cardiac Myocytes ◽

Rare Case ◽

Review Of Literature

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Hamartoma of mature cardiac myocytes: Report of a rare case with review of literature

Journal of the Practice of Cardiovascular Sciences ◽

10.4103/jpcs.jpcs_15_18 ◽

2018 ◽

Vol 4 (1) ◽

pp. 55

Author(s):

Sudheer Arava ◽

Kalpana Kumari ◽

Sanjeev Kumar ◽

Ashwani Bansal ◽

AkshayKumar Bisoi ◽

...

Keyword(s):

Cardiac Myocytes ◽

Rare Case ◽

Review Of Literature

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Identification of Pulmonary Deposits from a Sandpaper Worker: A Study by Electron Microscopy and X-Ray Microanalysis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053061 ◽

1982 ◽

Vol 40 ◽

pp. 52-53

Author(s):

S. K. Peng ◽

M.A. Egy ◽

J. K. Singh ◽

M.B. Bishop

Keyword(s):

Electron Microscopy ◽

Environmental Pollution ◽

Rare Case ◽

Interstitial Fibrosis ◽

X Ray ◽

Documented Case ◽

Etiologic Agents ◽

Pulmonary Interstitial Fibrosis ◽

Gold Miners ◽

Pulmonary Changes

Electron microscopy and energy dispersive x-ray microanalysis (EDXA) are found to be very useful tools for identification of etiologic agents in pneumoconiosis or interstitial pulmonary disorders. Pulmonary interstitial fibrosis and granulomatosis are frequently associated with occupational and environmental pollution. Numerous reports of pneumoconiosis in various occupations such as coal and gold miners are presented in the literature. However, there is no known documented case of pulmonary changes in workers in the sandpaper industry. This study reports a rare case of pulmonary granulomatosis containing deposits from abrasives of sandpaper diagnosed by using EDXA.

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Cardiac myocytes cultured on a basement membrane extract of the ehs tumor

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142979 ◽

1986 ◽

Vol 44 ◽

pp. 270-271

Author(s):

L. Terracio ◽

A. Dewey ◽

K. Rubin ◽

T.K. Borg

Keyword(s):

Extracellular Matrix ◽

Basement Membrane ◽

Cardiac Myocytes ◽

Normal Tissue ◽

Cell Spreading ◽

Collagen Iv ◽

Basement Membrane Extract ◽

Membrane Extract ◽

Growth Development ◽

Multicellular Organisms

The recognition and interaction of cells with the extracellular matrix (ECM) effects the normal physiology as well as the pathology of all multicellular organisms. These interactions have been shown to influence the growth, development, and maintenance of normal tissue function. In previous studies, we have shown that neonatal cardiac myocytes specifically interacts with a variety of ECM components including fibronectin, laminin, and collagens I, III and IV. Culturing neonatal myocytes on laminin and collagen IV induces an increased rate of both cell spreading and sarcomerogenesis.

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Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010015962x ◽

1990 ◽

Vol 48 (3) ◽

pp. 416-417

Author(s):

Jane K. Rosenthal ◽

Dianne L. Atkins ◽

William J. Marvin ◽

Penny A. Krumm

Keyword(s):

Cardiac Myocytes ◽

Structural Changes ◽

Three Dimensional ◽

Adrenergic Innervation ◽

Culture Cell ◽

Serial Sections ◽

Newborn Rats ◽

Primary Culture Cell ◽

Biological Studies ◽

Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

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