Comparative study of DNA damage and repair in head and neck cancer after radiation treatment

2009 ◽  
Vol 33 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Pawel Rusin ◽  
Jurek Olszewski ◽  
Alina Morawiec-Bajda ◽  
Karolina Przybylowska ◽  
Dariusz Kaczmarczyk ◽  
...  
Keyword(s):  
Dna Damage ◽  
Head And Neck Cancer ◽  
Comparative Study ◽  
Head And Neck ◽  
Neck Cancer ◽  
Radiation Treatment ◽  
Dna Damage And Repair

DNA Damage and Repair of Head and Neck Cancer Cells after Radio- and Chemotherapy

2009 ◽  
Vol 64 (7-8) ◽  
pp. 601-610 ◽  
Author(s):  
Pawel Rusin ◽  
Anna Walczak ◽  
Anita Zwierzchlejska ◽  
Jurek Olszewski ◽  
Alina Morawiec-Bajda ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer ◽  
Cancer Cell Line ◽  
Repair Capacity ◽  
Dna Damage And Repair ◽  
Γ Radiation

DNA repair is critical for successful chemo- and radiotherapy of human tumours, because their genotoxic sensitivity may vary in different types of cancer cells. In this study we have compared DNA damage and the efficiency of its repair after genotoxic treatment with hydrogen peroxide, cisplatin and γ-radiation of head and neck squamous cell carcinoma (HNSCC). Lymphocytes and tissue cells from biopsies of 37 cancer patients and 35 healthy donors as well as the HTB-43 larynx cancer cell line were employed. The cell sensitivity to genotoxic treatment was estimated by the MTT survival assay. The extent of DNA damage and efficiency of its repair was examined by the alkaline comet assay. Among the examined treatments, we found that HNSCC cells were the most sensitive to γ-radiation and displayed impaired DNA repair. In particular, DNA damage was repaired less effectively in cells from HNSCC metastasis than healthy controls. In conclusion, our results suggest that the different genotoxic sensitivity of HNSCC cells may depend on their DNA repair capacity what in turn may be connected with the effectiveness of head and neck cancer therapy.

scholarly journals DNA damage and repair in lymphocytes of normal individuals and cancer patients: studies by the comet assay and micronucleus tests.

2003 ◽  
Vol 50 (1) ◽  
pp. 181-190 ◽  
Author(s):  
Olena Palyvoda ◽  
Joanna Polańska ◽  
Andrzej Wygoda ◽  
Joanna Rzeszowska-Wolny
Keyword(s):  
Dna Damage ◽  
Head And Neck Cancer ◽  
Comet Assay ◽  
Cancer Patient ◽  
Head And Neck ◽  
Patient Group ◽  
Neck Cancer ◽  
Dna Damage And Repair ◽  
Damage Level ◽  
Healthy Donors

A population study is reported in which the DNA damage induced by g-radiation (2 Gy) and the kinetics of the subsequent repair were estimated by the comet and micronucleus assays in isolated lymphocytes of 82 healthy donors and patients with head and neck cancer before radiotherapy. The parameters of background and radiation-induced DNA damage, rate of repair, and residual non-repaired damage were measured by comet assay, and the repair kinetics for every donor were computer-fitted to an exponential curve. The level of background DNA damage before irradiation measured by comet assay as well as the level of micronuclei were significantly higher in the head and neck cancer patient group than in the healthy donors, while the parameters of repair were widely scattered in both groups. Cancer patient group contained significantly more individuals, whose irradiated lymphocytes showed high DNA damage, low repair rate and high non-repaired DNA damage level. Lymphocytes of donors belonging to this subgroup showed significantly lower inhibition of cell cycle after irradiation.

scholarly journals EGFR overexpression increases radiotherapy response in HPV-positive head and neck cancer through inhibition of DNA damage repair and HPV E6 downregulation

2021 ◽  
Vol 498 ◽  
pp. 80-97
Author(s):  
Elham Nafea Alsahafi ◽  
Selvam Thavaraj ◽  
Nazanin Sarvestani ◽  
Ofra Novoplansky ◽  
Moshe Elkabets ◽  
...  
Keyword(s):  
Dna Damage ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Dna Damage Repair ◽  
Hpv E6 ◽  
Damage Repair

scholarly journals Development of a Web-Based Supportive Care Program for Patients With Head and Neck Cancer

2020 ◽  
Vol 10 ◽  
Author(s):  
Carolyn Y. Fang ◽  
Thomas J. Galloway ◽  
Brian L. Egleston ◽  
Jessica R. Bauman ◽  
Barbara Ebersole ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Supportive Care ◽  
Head And Neck ◽  
Neck Cancer ◽  
Self Efficacy ◽  
Radiation Treatment ◽  
Web Based ◽  
Coping With Cancer ◽  
Wide Range ◽  
The Web

Patients undergoing radiation treatment for head and neck cancer experience significant side-effects that can impact a wide range of daily activities. Patients often report receiving insufficient information during and after treatment, which could impede rehabilitation efforts; they may also encounter practical and logistical barriers to receipt of supportive care. Thus, we developed a web-based program, My Journey Ahead, to provide information and strategies for managing symptom-focused concerns, which may be easily accessed from the patient’s home. The purpose of this study was to evaluate patient acceptability and satisfaction with the My Journey Ahead program. In Phase 1, five patients with head and neck squamous cell carcinoma (HNSCC) reviewed the web-based program and provided initial feedback, which informed program modifications. In Phase 2, 55 patients were recruited to evaluate the program. Patient assessments were obtained prior to and after use of the web-based program, and included measures of psychological distress, self-efficacy in coping with cancer-related issues, and satisfaction with the website. Among the 55 patients enrolled, 44 logged in and viewed the web-based program. Participants reported high levels of satisfaction with the information received, and indicated that the website was interesting and easy to use. Older age and higher levels of self-efficacy in coping were each associated with higher levels of satisfaction with the website. In summary, the web-based program was well-received by patients, the majority of whom found it to be informative and useful. An easy-to-use web-based program, particularly for older patients who may have difficulty locating reliable evidence-based information on the internet, may be helpful in addressing survivors’ needs in symptom management and coping with cancer.Clinical Trial Registrationhttps://clinicaltrials.gov/, NCT02442336

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