scholarly journals Cooperativity mediated by rationally selected combinations of human monoclonal antibodies targeting the henipavirus receptor binding protein

Cell Reports ◽  
2021 ◽  
Vol 36 (9) ◽  
pp. 109628
Author(s):  
Michael P. Doyle ◽  
Nurgun Kose ◽  
Viktoriya Borisevich ◽  
Elad Binshtein ◽  
Moushimi Amaya ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Receptor Binding ◽  
Binding Protein ◽  
Human Monoclonal Antibodies ◽  
Receptor Binding Protein

scholarly journals Functional cooperativity mediated by rationally selected combinations of human monoclonal antibodies targeting the henipavirus receptor binding protein

2021 ◽  
Author(s):  
Michael P. Doyle ◽  
Nurgun Kose ◽  
Viktoriya Borisevich ◽  
Elad Binshtein ◽  
Moushimi Amaya ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Receptor Binding ◽  
Binding Protein ◽  
Severe Disease ◽  
Hendra Virus ◽  
Human Monoclonal Antibodies ◽  
Human Mabs ◽  
Viral Attachment ◽  
Antigenic Sites ◽  
Receptor Binding Protein

AbstractHendra virus (HeV) and Nipah virus (NiV), the prototypic members of the Henipavirus (HNV) genus, are emerging, zoonotic paramyxoviruses known to cause severe disease across six mammalian orders, including humans (Eaton et al., 2006). While several research groups have made strides in developing candidate vaccines and therapeutics against henipaviruses, such countermeasures have not been licensed for human use, and significant gaps in knowledge about the human immune response to these viruses exist. To address these gaps, we isolated a large panel of human monoclonal antibodies (mAbs) from the B cells of an individual with prior occupation-related exposure to the equine HeV vaccine (Equivac® HeV). Competition-binding and hydrogen-deuterium exchange mass spectrometry (HDX-MS) studies identified at least six distinct antigenic sites on the HeV/NiV receptor binding protein (RBP) that are recognized by human mAbs. Antibodies recognizing multiple antigenic sites potently neutralized NiV and/or HeV isolates in vitro. The most potent class of cross-reactive antibodies achieved neutralization by blocking viral attachment to the host cell receptors ephrin-B2 and ephrin-B3. Antibodies from this class mimic receptor binding by inducing a receptor-bound conformation to the HeV-RBP protein tetramer, exposing an epitope that appears to lie hidden in the interface between protomers within the HeV-RBP tetramer. Antibodies that recognize this cryptic epitope potently neutralized HeV and NiV. Flow cytometric studies using cell-surface-displayed HeV-RBP protein showed that cross-reactive, neutralizing mAbs from each of these classes cooperate for binding. In a highly stringent hamster model of NiVB infection, antibodies from both classes reduced morbidity and mortality and achieved synergistic protection in combination and provided therapeutic benefit when combined into two bispecific platforms. These studies identified multiple candidate mAbs that might be suitable for use in a cocktail therapeutic approach to achieve synergistic antiviral potency and reduce the risk of virus escape during treatment.

Isolation of steroid receptor binding protein from chicken oviduct and production of monoclonal antibodies

Biochemistry ◽  
1985 ◽  
Vol 24 (15) ◽  
pp. 4214-4222 ◽  
Author(s):  
William P. Sullivan ◽  
Benjamin T. Vroman ◽  
Vickie J. Bauer ◽  
Raj K. Puri ◽  
Robert M. Riehl ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Receptor Binding ◽  
Binding Protein ◽  
Steroid Receptor ◽  
Chicken Oviduct ◽  
Receptor Binding Protein

Localization of the growth hormone receptor/binding protein in skin

1990 ◽  
Vol 126 (3) ◽  
pp. 467-NP ◽  
Author(s):  
P. E. Lobie ◽  
W. Breipohl ◽  
D. T. Lincoln ◽  
J. García-Aragón ◽  
M. J. Waters
Keyword(s):  
Monoclonal Antibodies ◽  
Receptor Binding ◽  
Growth Hormone Receptor ◽  
Binding Protein ◽  
Cell Types ◽  
Myoepithelial Cells ◽  
Hair Follicles ◽  
Sweat Glands ◽  
Gh Receptor ◽  
Receptor Binding Protein

ABSTRACT Acromegaly is characterized by coarsening of facial features, acanthosis nigricans, hypertrichosis and oily skin. To determine the site through which GH exerts these effects, we have used immunohistochemistry to localize the GH receptor/binding protein (BP) in rat, rabbit and human skin. Three monoclonal antibodies (MAb 1, 43, 263) were immunoreactive in identical locations, whereas no immunoreactivity was evident when control monoclonal antibodies (MAb 50.8 and MAb 7 (rat)) were used. Skin from neonatal and adult animals was used to determine whether GH receptor/BP expression was developmentally regulated. Immunoreactivity of the GH receptor/BP in the three species was consistently localized in the stratum basale and stratum spinosum. Intermittent staining was observed in the stratum granulosum. Scattered basal epidermal cells often displayed more intense immunoreactivity. This distribution was observed at all maturational stages examined. Intense GH receptor/BP immunoreactivity was observed in all histological layers of the lower one-third of hair follicles and in hair matrix cells of the dermal papillae. Immunoreactivity was also detected in the outer epithelial root sheath of the upper two-thirds of hair follicles, in sebaceous glands and in fibroblasts of the connective tissue sheath surrounding the follicle. GH receptor/BP immunoreactivity was also present in the secretory duct and myoepithelial cells of human eccrine sweat glands. Fibroblasts, Schwann cells of peripheral nerve fascicles, skeletal muscle cells and adipocytes of the dermis were also immunoreactive as were medial smooth muscle and endothelial cells of arteries. These results provide evidence that GH acts locally on the epidermis and epidermal appendages concordant with our recent localization of GH receptor/BP to epithelial cell types of the gastrointestinal and reproductive systems. Journal of Endocrinology (1990) 126, 467–472

scholarly journals F1Aα, a Death Receptor-binding Protein Homologous to theCaenorhabditis elegansSex-determining Protein, FEM-1, Is a Caspase Substrate That Mediates Apoptosis

1999 ◽  
Vol 274 (45) ◽  
pp. 32461-32468 ◽  
Author(s):  
Shing-Leng Chan ◽  
Kuan-Onn Tan ◽  
Li Zhang ◽  
Karen S. Y. Yee ◽  
Francesca Ronca ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Binding Protein ◽  
Death Receptor ◽  
Caspase Substrate ◽  
Receptor Binding Protein

Specific detection of Campylobacter jejuni using the bacteriophage NCTC 12673 receptor binding protein as a probe

The Analyst ◽  
2011 ◽  
Vol 136 (22) ◽  
pp. 4780 ◽  
Author(s):  
Amit Singh ◽  
Denis Arutyunov ◽  
Mark T. McDermott ◽  
Christine M. Szymanski ◽  
Stephane Evoy
Keyword(s):  
Campylobacter Jejuni ◽  
Receptor Binding ◽  
Binding Protein ◽  
Specific Detection ◽  
Receptor Binding Protein

Receptor binding protein amperometric affinity sensor for rapid β-lactam quantification in milk

1999 ◽  
Vol 398 (1) ◽  
pp. 13-22 ◽  
Author(s):  
S.J. Setford ◽  
R.M. Van Es ◽  
Y.J. Blankwater ◽  
S. Kröger
Keyword(s):  
Receptor Binding ◽  
Binding Protein ◽  
Receptor Binding Protein

scholarly journals A receptor-binding protein ofCampylobacter jejunibacteriophage NCTC 12673 recognizes flagellin glycosylated with acetamidino-modified pseudaminic acid

2014 ◽  
Vol 95 (1) ◽  
pp. 101-115 ◽  
Author(s):  
Muhammad Afzal Javed ◽  
Lieke B. van Alphen ◽  
Jessica Sacher ◽  
Wen Ding ◽  
John Kelly ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Binding Protein ◽  
Pseudaminic Acid ◽  
Receptor Binding Protein
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