Biogenic gas is an important end-product of methanogenesis in an anoxic environment. Based on the coal, coal-gangue, mine water of Songzao Coal Mine and in the case of adding inoculums, the quantities of gas and the contents of methane are measured by the microorganism action, and also respectively measured fermentation broth and residues by NO3--N, NH4+-N, S2- , SO42-, the values of pH, metals and the organic matter. The results show that coal and coal-gangue are involved in the microbial role; the quantity of inoculums is directly related to the quantities of gas and the contents of methane, at the same time it has a certain degree of impact on the contents of NO3--N, NH4+ -N, S2- , SO42- and metals; besides, because of the microbial anaerobic fermentation, the contents of NH3+ are seriously substandard; overall, the group B which has a smaller proportion of coal-gangue has a less impact to the environment compared to the group A which has a larger proportion of coal-gangue. In the further research into the direction of organic matter and obtaining and using the biogas, we must prevent secondary pollution of groundwater at the same time.
