Genotoxic and cytotoxic effects of polyethylene microplastics on human peripheral blood lymphocytes

Chemosphere ◽  
2021 ◽  
Vol 272 ◽  
pp. 129805 ◽  
Author(s):  
Hayal Çobanoğlu ◽  
Murat Belivermiş ◽  
Ercan Sıkdokur ◽  
Önder Kılıç ◽  
Akın Çayır
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Cytotoxic Effects ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

scholarly journals Evaluation of Genotoxic and Cytotoxic Effects in Human Peripheral Blood Lymphocytes ExposedIn Vitroto Neonicotinoid Insecticides News

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
María Elena Calderón-Segura ◽  
Sandra Gómez-Arroyo ◽  
Rafael Villalobos-Pietrini ◽  
Carmen Martínez-Valenzuela ◽  
Yolanda Carbajal-López ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blue Dye ◽  
Cytotoxic Effects ◽  
Blood Lymphocytes ◽  
Neonicotinoid Insecticides ◽  
Human Peripheral Blood Lymphocytes

Calypso (thiacloprid), Poncho (clothianidin), Gaucho (imidacloprid), and Jade (imidacloprid) are commercial neonicotinoid insecticides, a new class of agrochemicals in México. However, genotoxic and cytotoxic studies have not been performed. In the present study, human peripheral blood lymphocytes (PBL) were exposedin vitroto different concentrations of the four insecticides. The genotoxic and cytotoxic effects were evaluated using the alkaline comet and trypan blue dye exclusion assays. DNA damage was evaluated using two genotoxicity parameters: tail length and comet frequency. Exposure to9.5×10-6to5.7×10-5 M Jade;2.8×10-4to1.7×10-3 M Gaucho;0.6×10-1to1.4×10-1 M Calypso;1.2×10-1to9.5×10-1 M Poncho for 2 h induced a significant increase DNA damage with a concentration-dependent relationship. Jade was the most genotoxic of the four insecticides studied. Cytotoxicity was observed in cells exposed to18×10-3 M Jade,2.0×10-3 M Gaucho,2.0×10-1 M Calypso, 1.07 M Poncho, and cell death occurred at30×10-3 M Jade,3.3×10-3 M Gaucho,2.8×10-1 M Calypso, and 1.42 M Poncho. This study provides the first report of genotoxic and cytotoxic effects in PBL followingin vitroexposure to commercial neonicotinoid insecticides.

Genotoxic and cytotoxic effects of flumetralin in human peripheral blood lymphocytes in vitro

2016 ◽  
Vol 32 (12) ◽  
pp. 1927-1934 ◽  
Author(s):  
Ayşe Yavuz Kocaman ◽  
Sevcan Bucak
Keyword(s):  
Treatment Period ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Herbicidal Activity ◽  
Cytotoxic Effects ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Human Pbls

Flumetralin, a synthetic plant growth regulator with herbicidal activity belonging to the 2,6-dinitroaniline class of chemicals, has been evaluated for its ability to induce genotoxicity in human peripheral blood lymphocytes (PBLs). The potential genotoxic and cytotoxic effects of flumetralin were investigated in vitro by chromosome aberration (CA) and cytokinesis-block micronucleus assays. Human PBLs were treated with 125, 250, 500, and 1000 µg/mL flumetralin for 24 and 48 h. Flumetralin statistically significantly increased the frequency of structural CAs at the three highest concentrations (250, 500, and 1000 µg/mL) for both treatment periods (24 and 48 h) when compared with both the negative and solvent controls. In addition, micronucleus formation was significantly induced at higher concentrations (250, 500, and 1000 µg/mL) for 24 h and at 125 and 500 µg/mL of flumetralin for the 48-h treatment period compared with the controls. Because of the excessive cytostatic effects of flumetralin, binuclear cells could not be detected sufficiently at the highest two concentrations (500 and 1000 µg/mL) for the 48-h treatment period. Furthermore, flumetralin significantly decreased the mitotic index and nuclear division index for all concentrations and treatment times compared with the control groups. The present results indicate that flumetralin was clastogenic and cytotoxic/cytostatic to human PBLs. This study presents the first report of the genotoxic and cytotoxic properties of flumetralin.

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