Influence of the sample-solvent on protein retention, mass transfer and unfolding kinetics in hydrophobic interaction chromatography

2010 ◽  
Vol 1217 (17) ◽  
pp. 2812-2820 ◽  
Author(s):  
Renata Muca ◽  
Wojciech Marek ◽  
Wojciech Piątkowski ◽  
Dorota Antos
Keyword(s):  
Mass Transfer ◽  
Hydrophobic Interaction ◽  
Hydrophobic Interaction Chromatography ◽  
Protein Retention ◽  
Unfolding Kinetics

Prediction of protein retention times in hydrophobic interaction chromatography by robust statistical characterization of their atomic-level surface properties

2016 ◽  
Vol 32 (2) ◽  
pp. 372-381 ◽  
Author(s):  
Alexander T. Hanke ◽  
Marieke E. Klijn ◽  
Peter D. E. M. Verhaert ◽  
Luuk A. M. van der Wielen ◽  
Marcel Ottens ◽  
...  
Keyword(s):  
Surface Properties ◽  
Hydrophobic Interaction ◽  
Hydrophobic Interaction Chromatography ◽  
Atomic Level ◽  
Level Surface ◽  
Statistical Characterization ◽  
Protein Retention

scholarly journals A Simple Method for the Estimation of Protein Retention in Hydrophobic Interaction Chromatography Under Different Operation Conditions

2007 ◽  
Vol 1 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Andrea Mahn ◽  
M. Elena Lienqueo ◽  
Juan A. Asenjo
Keyword(s):  
Hydrophobic Interaction ◽  
Linear Correlation ◽  
Retention Time ◽  
Process Design ◽  
Scale Up ◽  
Hydrophobic Interaction Chromatography ◽  
Simple Method ◽  
Protein Retention ◽  
Operation Conditions

Protein behavior in Hydrophobic Interaction Chromatography using different chromatographic conditions was investigated. A linear correlation was found between protein retention time on different matrixes and different initial elution salt concentrations. Mathematical correlations between retention times under different chromatographic conditions were obtained and validated, which can be used in process design and scale-up.

New approaches for predicting protein retention time in hydrophobic interaction chromatography

2006 ◽  
Vol 19 (4) ◽  
pp. 260-269 ◽  
Author(s):  
M. E. Lienqueo ◽  
A. Mahn ◽  
G. Navarro ◽  
J. C. Salgado ◽  
T. Perez-Acle ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Retention Time ◽  
Hydrophobic Interaction Chromatography ◽  
Protein Retention ◽  
New Approaches
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