Isolation of monoclonal antibody from a Chinese hamster ovary supernatant. II: Dynamics of the integrated separation on ion exchange and hydrophobic interaction chromatography media

2013 ◽  
Vol 1305 ◽  
pp. 64-75 ◽  
Author(s):  
Wojciech Marek ◽  
Renata Muca ◽  
Sylwia Woś ◽  
Wojciech Piątkowski ◽  
Dorota Antos
Keyword(s):  
Monoclonal Antibody ◽  
Ion Exchange ◽  
Hydrophobic Interaction ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Hydrophobic Interaction Chromatography

scholarly journals Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

10.3791/58947 ◽  
2019 ◽  
Author(s):  
Sai Rashmika Velugula-Yellela ◽  
David N. Powers ◽  
Phillip Angart ◽  
Anneliese Faustino ◽  
Talia Faison ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Purification, immobilization and characterization of thermostable α-amylase from a thermophilic bacterium Geobacillus sp. TF14

2017 ◽  
Vol 42 (6) ◽  
Author(s):  
Şaban Keskin ◽  
Nagihan Saglam Ertunga
Keyword(s):  
Ion Exchange ◽  
Hydrophobic Interaction ◽  
Hydrophobic Interaction Chromatography ◽  
Maximal Activity ◽  
Thermophilic Bacterium ◽  
Single Band ◽  
Sds Page

AbstractObjective:In this study, α-amylase from a thermophilic bacterium Geobacillus sp. TF14 was purified and immobilized on two different supports.Methods:Ion exchange and hydrophobic interaction chromatography techniques were employed for the purification.Results:The enzyme was purified as 17.11 fold and determined as a single band of 54 kDa on SDS-PAGE. Purified enzyme showed two pH optimums of pH 5.00 and pH 9.00 and the enzyme is quite stable at these pHs over a period of 48 h. Purified enzyme showed maximal activity at 75°C and stability at this temperature over a period of 72 h. It was observed that CaConclusion:It can be concluded that the purified enzyme may find application in many fields of starch based industries.

The use of catechins in Chinese hamster ovary cell media for the improvement of monoclonal antibody yields and a reduction of acidic species

2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Luis Toronjo‐Urquiza ◽  
Adelina E. Acosta‐Martin ◽  
David C. James ◽  
Tibor Nagy ◽  
Robert J. Falconer
Keyword(s):  
Monoclonal Antibody ◽  
Chinese Hamster Ovary Cell ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell

Efficient enrichment of high-producing recombinant Chinese hamster ovary cells for monoclonal antibody by flow cytometry

2015 ◽  
Vol 120 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Takeshi Okumura ◽  
Kenji Masuda ◽  
Kazuhiko Watanabe ◽  
Kenji Miyadai ◽  
Koichi Nonaka ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells
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