Ultra high efficiency/low pressure supercritical fluid chromatography with superficially porous particles for triglyceride separation

Until less than 10 years ago, chiral separations were carried out with columns packed with 5 or 3 μ m fully porous particles (FPPs). Times to resolve enantiomeric mixtures were easily larger than 30 min, or so. Pushed especially by stringent requirements from medicinal and pharmaceutical industries, during the last years the field of chiral separations by liquid chromatography has undergone what can be defined a “true revolution”. With the purpose of developing ever faster and efficient method of separations, indeed, very efficient particle formats, such as superficially porous particles (SPPs) or

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Evaluation of stationary phases packed with superficially porous particles for the analysis of pharmaceutical compounds using supercritical fluid chromatography

Journal of Chromatography A ◽

10.1016/j.chroma.2014.07.078 ◽

2014 ◽

Vol 1360 ◽

pp. 275-287 ◽

Cited By ~ 37

Author(s):

Alexandre Grand-Guillaume Perrenoud ◽

William P. Farrell ◽

Christine M. Aurigemma ◽

Nicole C. Aurigemma ◽

Szabolcs Fekete ◽

...

Keyword(s):

Supercritical Fluid ◽

Supercritical Fluid Chromatography ◽

Stationary Phases ◽

Pharmaceutical Compounds ◽

Porous Particles ◽

Superficially Porous Particles

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Ultra high efficiency/low pressure supercritical fluid chromatography (UHE/LP‐SFC) for triglyceride analysis: Identification, quantification, and classification of vegetable oils

Analytical Science Advances ◽

10.1002/ansa.202000156 ◽

2021 ◽

Vol 2 (1-2) ◽

pp. 33-42

Author(s):

Eric Lesellier ◽

Anna Latos ◽

Caroline West

Keyword(s):

Supercritical Fluid ◽

Vegetable Oils ◽

Supercritical Fluid Chromatography ◽

High Efficiency ◽

Low Pressure ◽

Triglyceride Analysis

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Advantages and Drawbacks of Popular Supercritical Fluid Chromatography / Mass Spectrometry Interfacing Approaches—A User's Perspective

European Journal of Mass Spectrometry ◽

10.1255/ejms.731 ◽

2005 ◽

Vol 11 (2) ◽

pp. 189-197 ◽

Cited By ~ 32

Author(s):

J. David Pinkston

Keyword(s):

Mass Spectrometry ◽

Supercritical Fluid ◽

Supercritical Fluid Chromatography ◽

High Speed ◽

High Performance ◽

High Efficiency ◽

User Friendliness ◽

Chromatographic Separations ◽

Bioanalytical Applications ◽

Column Pressure

Mobile phases in supercritical fluid chromatography (SFC) have low viscosities and high diffusion coefficients with respect to those of traditional high-performance liquid chromatography (HPLC). These properties allow higher mobile phase flow rates and/or longer columns in SFC, resulting in rapid analyses and high efficiency separations. In addition, chiral SFC is becoming especially popular. Mass spectrometry (MS) is arguably the most popular “informative” detector for chromatographic separations. Most SFC/MS is performed with atmospheric pressure ionization (API) sources. Unlike LC/MS, the interface between the SFC column and the API source must allow control of the downstream (post-column) pressure while also providing good chromatographic fidelity. Here, we compare and contrast the popular interfacing approaches. Some are simple, such as direct effluent introduction with no active back pressure regulator (BPR) in high-speed bioanalytical applications. The pressure-regulating fluid interface is more versatile and provides excellent chromatographic fidelity, but is less user friendly. The pre-BPR-split interface and an interface which provides total flow introduction with a mechanical BPR are good compromises between user friendliness and performance and have become the most popular among practitioners. Applications of SFC/MS using these various interfaces are also discussed.

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