Imidazole-free purification of His 3 -tagged recombinant proteins using ssDNA aptamer-based affinity chromatography

2015 ◽  
Vol 1418 ◽  
pp. 130-139 ◽  
Author(s):  
Filip Bartnicki ◽  
Ewa Kowalska ◽  
Katarzyna Pels ◽  
Wojciech Strzalka
Keyword(s):  
Affinity Chromatography ◽  
Recombinant Proteins ◽  
Ssdna Aptamer

scholarly journals Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

RSC Advances ◽  
2019 ◽  
Vol 9 (20) ◽  
pp. 11038-11045
Author(s):  
Sergio G. Hernandez-Leon ◽  
Jose A. Sarabia-Sainz ◽  
Gabriela Ramos-Clamont Montfort ◽  
José Ángel Huerta-Ocampo ◽  
Ana M. Guzman-Partida ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Affinity Chromatography ◽  
Silica Nanoparticles ◽  
Recombinant Proteins ◽  
Iminodiacetic Acid ◽  
Immobilized Metal Affinity Chromatography ◽  
Core Shell ◽  
Metal Affinity ◽  
Chemically Modified ◽  
High Molecular Weight Proteins

Silica nanoparticles were synthesized and chemically modified with iminodiacetic acid (IDA) and Ni2+ ions surrounded by a bis-acrylamide polymeric shell to obtain a new core–shell immobilized metal affinity chromatography (IMAC) based material.

Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatography

2016 ◽  
Vol 29 (9) ◽  
pp. 436-445 ◽  
Author(s):  
Mojtaba Hedayati Ch ◽  
Jafar Amani ◽  
Hamid Sedighian ◽  
Mohsen Amin ◽  
Jafar Salimian ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Ssdna Aptamer ◽  
Enterotoxin B ◽  
Sepharose 4B
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