Studies with an immobilized metal affinity chromatography cassette system involving binuclear triazacyclononane-derived ligands: Automation of batch adsorption measurements with tagged recombinant proteins

2014 ◽  
Vol 1351 ◽  
pp. 61-69 ◽  
Author(s):  
Martin Petzold ◽  
Campbell J. Coghlan ◽  
Milton T.W. Hearn
Keyword(s):  
Affinity Chromatography ◽  
Recombinant Proteins ◽  
Immobilized Metal Affinity Chromatography ◽  
Batch Adsorption ◽  
Metal Affinity

scholarly journals Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

RSC Advances ◽  
2019 ◽  
Vol 9 (20) ◽  
pp. 11038-11045
Author(s):  
Sergio G. Hernandez-Leon ◽  
Jose A. Sarabia-Sainz ◽  
Gabriela Ramos-Clamont Montfort ◽  
José Ángel Huerta-Ocampo ◽  
Ana M. Guzman-Partida ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Affinity Chromatography ◽  
Silica Nanoparticles ◽  
Recombinant Proteins ◽  
Iminodiacetic Acid ◽  
Immobilized Metal Affinity Chromatography ◽  
Core Shell ◽  
Metal Affinity ◽  
Chemically Modified ◽  
High Molecular Weight Proteins

Silica nanoparticles were synthesized and chemically modified with iminodiacetic acid (IDA) and Ni2+ ions surrounded by a bis-acrylamide polymeric shell to obtain a new core–shell immobilized metal affinity chromatography (IMAC) based material.

Immobilized metal-ion affinity systems for recovery and structure–function studies of proteins at molecular, supramolecular, and cellular levels

2010 ◽  
Vol 82 (1) ◽  
pp. 39-55 ◽  
Author(s):  
Rajasekar R. Prasanna ◽  
Mookambeswaran A. Vijayalakshmi
Keyword(s):  
Capillary Electrophoresis ◽  
Recombinant Proteins ◽  
Gel Electrophoresis ◽  
Metal Ion ◽  
Immobilized Metal Affinity Chromatography ◽  
Metal Affinity ◽  
Purification Technique ◽  
Affinity Gel Electrophoresis ◽  
Insight Into ◽  
Histidine Tag

Immobilized metal-ion affinity (IMA) adsorption is a collective term that is used to include all kinds of adsorptions where the metal ion serves as the characteristic and most essential part of adsorption center. Of all the IMA techniques, immobilized metal-affinity chromatography (IMAC) has been gaining popularity as the choice of purification technique for proteins. IMAC represents a separation technique that is primarily useful for proteins with natural surface exposed-histidine residues and for recombinant proteins with engineered histidine tag. This review also gives insight into other nonchromatographic applications of IMA adsorption such as immobilized metal-ion affinity gel electrophoresis (IMAGE), immobilized metal-ion affinity capillary electrophoresis (IMACE), and immobilized metal-ion affinity partitioning (IMAP).

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