Enantioseparation performance of superficially porous particle vancomycin-based chiral stationary phases in supercritical fluid chromatography and high performance liquid chromatography; applicability for psychoactive substances

2021 ◽  
Vol 1637 ◽  
pp. 461846
Author(s):  
Denisa Folprechtová ◽  
Květa Kalíková ◽  
Kian Kadkhodaei ◽  
Cornelia Reiterer ◽  
Daniel W. Armstrong ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Porous Particle ◽  
Psychoactive Substances

scholarly journals Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2425 ◽  
Author(s):  
Tsunehisa Hirose ◽  
Daniel Keck ◽  
Yoshihiro Izumi ◽  
Takeshi Bamba
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Retention Factor ◽  
Normal Phase ◽  
Retention Behavior ◽  
Selection Of

The retention behavior of a wide variety of stationary phases was compared in supercritical fluid chromatography (SFC) and normal-phase high-performance liquid chromatography (NP-HPLC). We also attempted to elucidate the retention behavior in SFC by investigating the selectivity of the different stationary phases. SFC separation conditions with polar stationary phases, such as silica gel (SL) and diol (Diol) phases, operate via adsorptions that include hydrophilic and ionic interactions similar to those in NP-HPLC. Moreover, non-polar stationary phases, such as pentabromophenyl (PBr), pyrenylethyl (PYE), and octadecyl (C18), could be used despite the non-polar mobile phase conditions, because the dispersion and π-π interactions were stronger in SFC than in HPLC. These results reflect the selectivity of the stationary phase and its retention factor, thus providing useful information for the selection of appropriate stationary phases for particular analytes.

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