Evaluation of highly sensitive indigenous milk ELISA kit with fecal culture, milk culture and fecal-PCR for the diagnosis of bovine Johne's disease (BJD) in India

2007 ◽  
Vol 30 (3) ◽  
pp. 175-186 ◽  
Author(s):  
S.V. Singh ◽  
A.V. Singh ◽  
R. Singh ◽  
K.S. Sandhu ◽  
P.K. Singh ◽  
...  
Keyword(s):  
Johne's Disease ◽  
Johne’S Disease ◽  
Fecal Culture ◽  
Elisa Kit ◽  
Highly Sensitive ◽  
Milk Elisa

scholarly journals Evaluation of Result Variability with a Commercial Johne's Disease Enzyme-Linked Immunosorbent Assay Kit and Repeat Testing of Samples

2002 ◽  
Vol 14 (5) ◽  
pp. 423-426 ◽  
Author(s):  
John M. Adaska ◽  
Claudia A. Muñoz-Zanzi ◽  
Sharon K. Hietala
Keyword(s):  
Immunosorbent Assay ◽  
Coefficient Of Variation ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Large Animal ◽  
Test Results ◽  
Elisa Kit ◽  
Individual Test

Two hundred one serum samples from individual dairy cows with a range of results on initial testing with a commercial Johne's disease enzyme-linked immunosorbent assay (ELISA) kit were repeat tested 5 times in each of 2 laboratories with kits produced by the same manufacturer. The results for the samples with all 10 replicates showed that the values for individual samples often had a coefficient of variation greater than 20%. As expected, the standard deviation for the results increased as the average value increased and the coefficient of variation was greater in samples with low mean values. The different lots of the commercial ELISA kit used in this study had a significant effect on both the optical density and the calculated sample to positive (S/P) ratio for test replicates. Based on the variability detected in S/P ratios of replicate samples, application of a single cutoff point to interpret individual test results as positive or negative for antibodies to Mycobacterium avium subspecies paratuberculosis could result in inconsistent classification of animals as positive or negative for Johne's disease. Such inconsistency in test interpretation leads to frustration in large animal veterinarians or producers trying to make management decisions based on individual test results. Instead of dichotomizing the test results as positive or negative based on a single cutoff value, reporting numerical values and supplying a classification scheme that includes a suspect category reflecting the uncertainty inherent in the test is recommended to provide more reliable result interpretation.

scholarly journals Genetic parameters of milk ELISA scores for Johne's disease

2010 ◽  
Vol 93 (4) ◽  
pp. 1729-1735 ◽  
Author(s):  
S.A. Attalla ◽  
A.J. Seykora ◽  
J.B. Cole ◽  
B.J. Heins
Keyword(s):  
Genetic Parameters ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Milk Elisa

Evaluation of indigenous milk ELISA with m-culture and m-PCR for the diagnosis of Bovine Johne’s disease (BJD) in lactating Indian dairy cattle

2008 ◽  
Vol 84 (1) ◽  
pp. 30-37 ◽  
Author(s):  
G. Sharma ◽  
S.V. Singh ◽  
I. Sevilla ◽  
A.V. Singh ◽  
R.J. Whittington ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Milk Elisa

Development of New ‘Indigenous Dot-ELISA Kit’ as Sensitive Field Based Herd Screening Test for the Diagnosis of Johne’s Disease in the Domestic Buffalo Population

2015 ◽  
Vol 11 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Shoor Vir Singh ◽  
Sachin Digambar Audarya ◽  
Manju Singh ◽  
Bjorn John Stephen ◽  
Daljeet Chhabra ◽  
...  
Keyword(s):  
Screening Test ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Elisa Kit ◽  
Dot Elisa

scholarly journals Individual and herd-level milk ELISA test status for Johne's disease in Ireland after correcting for non-disease-associated variables

2020 ◽  
Vol 103 (10) ◽  
pp. 9345-9354
Author(s):  
Conor G. McAloon ◽  
Luke O'Grady ◽  
Bruno Botaro ◽  
Simon J. More ◽  
Michael Doherty ◽  
...  
Keyword(s):  
Elisa Test ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Herd Level ◽  
Milk Elisa ◽  
Associated Variables

scholarly journals Sero-Prevalence of Paratuberculosis (Johne's Disease) in Cattle Population of South-Western Bangalore Using ELISA Kit

2012 ◽  
Vol 02 (04) ◽  
pp. 196-200 ◽  
Author(s):  
Anvita Gupta ◽  
Sobha M. Rani ◽  
Pushpa Agrawal ◽  
Praveen Kumar Gupta
Keyword(s):  
Johne's Disease ◽  
Johne’S Disease ◽  
Cattle Population ◽  
Elisa Kit
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