Detection of immunoglobulin response to COVID-19 vaccination using a novel rapid fingerstick assay

Electronic cigarette (e-cigarette) use has had an exponential increase in popularity since the product was released to the public. Currently, there is a lack of human studies that assess different biomarker levels. This pilot study attempts to link e-cigarette and other tobacco product usage with clinical respiratory symptoms and immunoglobulin response. Subjects completed surveys in order to collect self-reported data on tobacco product flavor preferences. Along with this, plasma samples were collected to test for immunoglobulin G (IgG) and E (IgE) levels. Our pilot study’s cohort had a 47.9% flavor preference towards fruit flavors and a 63.1% preference to more sweet flavors. E-cigarette and traditional cigarette smokers were the two subject groups to report the most clinical symptoms. E-cigarette users also had a significant increase in plasma IgE levels compared to non-tobacco users 1, and dual users had a significant increase in plasma IgG compared to non-tobacco users 2, cigarette smokers, and waterpipe smokers. Our pilot study showed that users have a preference toward fruit and more sweet flavors and that e-cigarette and dual use resulted in an augmented systemic immune response.

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Immunoglobulin response of rabbits infected with malignant catarrhal fever virus

Research in Veterinary Science ◽

10.1016/s0034-5288(18)32371-3 ◽

1982 ◽

Vol 33 (1) ◽

pp. 120-122 ◽

Cited By ~ 5

Author(s):

P.B. Rossiter

Keyword(s):

Fever Virus ◽

Malignant Catarrhal Fever ◽

Immunoglobulin Response

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The specific immunoglobulin response in cattle immunized with isolated Theileria parva antigens

Parasitology ◽

10.1017/s0031182000046163 ◽

1974 ◽

Vol 69 (1) ◽

pp. 43-53 ◽

Cited By ~ 24

Author(s):

G. G. Wagner ◽

W. P. H. Duffus ◽

M. J. Burridge

Keyword(s):

Indirect Immunofluorescence ◽

Specific Antibody ◽

Complement Fixation ◽

Post Inoculation ◽

Theileria Parva ◽

Serum Samples ◽

Indirect Haemagglutination ◽

Specific Immunoglobulin ◽

Immunoglobulin Response

Cattle were inoculated with purified Theileria parva piroplasm and/or schizont antigen. Two similar inoculations were given 10 days apart. Serum samples were regularly collected and the indirect haemagglutination (IHA), indirect immunofluorescence (IFA), complement-fixation (CF) and immunodiffusion (ID) tests were used to detect T. parva antibodies. Selected sera were separated by Sephadex G 200 and fractions examined for specific immunoglobulin activity.With the IHA test specific antibody first appeared 4 days post inoculation (dpi) and reached high titres by 8 dpi. With the IFA and CF tests specific antibody also appeared 4 dpi and reached high titres by 8–14 and 10–14 dpi respectively. Specific ID activity was detected as early as 6 dpi and persisted for the length of the experiment.On fractionation of the sera both the IHA and CF tests indicated a sequential production of T. parva IgM and 7S Ig. However, in cattle inoculated with schizont antigen only, a significant 7S Ig component was not detected by the IHA test until 16 dpi, but was demonstrated by the CF test 7 dpi. The IFA test, however, detected anti T. parva activity almost exclusively in the 7S Ig fractions. All cattle were challenged 35–42 dpi with infective T. parva stabilate and all cattle proved fully susceptible. The lack of protection by high titres of specific T. parva immunoglobulin is discussed, together with the data on the differential immunoglobulin response, in relation to previous results.

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