Accreditation of HLA tests and an external quality control program

2009 ◽  
Vol 42 (4-5) ◽  
pp. 320
Author(s):  
Fatma Savran Oguz
2002 ◽  
Vol 44 (5) ◽  
pp. 431-436 ◽  
Author(s):  
Ana Flisser ◽  
Felipe García-Malo ◽  
María de los Angeles Canepa ◽  
Susana Doncel ◽  
Raquel Espinoza ◽  
...  

1980 ◽  
Vol 26 (11) ◽  
pp. 1607-1609 ◽  
Author(s):  
S P Haynes ◽  
J M Corcoran ◽  
C J Eastman ◽  
F A Doy

Abstract We describe a rapid, precise radiommunoassay for progesterone in 25 muL of unextracted serum. Progesterone is released from its binding protein by adding an optimal amount of cortisol, which binds to the same protein (cortisol binding globulin) as progesterone. The amount of cortisol required does not cross react with the specific progesterone antibody used. This approach considerably shortens assay time and removes a tedious and imprecise stage in the conventional assay of serum progesterone. Results correlated well (r = 0.97) with a method involving organic solvent extraction of progesterone from serum. During the two years we have used this method in a busy diagnostic endocrine laboratory, the between-assay precision (CV) for low-, medium-, and high-concentration quality control sera was 12, 7, and 9%, respectively. Data from participation in an independent external quality-control program verified the adequacies of the method.


EP Europace ◽  
2009 ◽  
Vol 12 (2) ◽  
pp. 210-215 ◽  
Author(s):  
B. Nowak ◽  
B. Misselwitz ◽  
A. Erdogan ◽  
R. Funck ◽  
W. Irnich ◽  
...  

1985 ◽  
Vol 31 (7) ◽  
pp. 1158-1162 ◽  
Author(s):  
R W Jenny

Abstract Serum specimens with concentrations simulating an overdose of salicylate and acetaminophen were submitted to laboratories participating in an external quality-control program, to evaluate both the magnitude of salicylate interference in colorimetric acetaminophen methods and the clinical significance of the interference. The apparent acetaminophen concentration determined by nitration methods was increased by about 0.70 mg/L per milligram of salicylate per deciliter. Of those laboratories using nitration procedures, 25% do not routinely correct for salicylate and 66% use the (incorrect) correction factor provided by a kit manufacturer. Laboratory data, as they would have been reported to physicians, were used to estimate the acetaminophen half-life and were also applied to a nomogram used to assess the probability of hepatotoxicity. Interference by salicylate in the simulated overdose of 10 g (total dose) of each drug falsely indicated impending hepatic necrosis unless the appropriate correction factor was used. Laboratories using nitration procedures should screen samples submitted for acetaminophen assay for the presence of salicylate and, if present, either use a method specific for acetaminophen or utilize a correction factor determined in-house.


2015 ◽  
Vol 60 (1) ◽  
pp. 632-636 ◽  
Author(s):  
Maria Siopi ◽  
Efthymios Neroutsos ◽  
Kalliopi Zisaki ◽  
Maria Gamaletsou ◽  
Maria Pirounaki ◽  
...  

ABSTRACTVoriconazole levels were determined with high-performance liquid chromatography (HPLC) and a microbiological agar diffusion assay using aCandida parapsilosisisolate in 103 serum samples from an HPLC-tested external quality control program (n= 39), 21 patients receiving voriconazole monotherapy (n= 39), and 7 patients receiving combination therapy (n= 25). The results of the bioassay were correlated with the results obtained from the external quality control program samples and with the HPLC results in sera from patients on voriconazole monotherapy and on combination therapy with an echinocandin (Spearman's rank correlation coefficient [rs], > 0.93; mean ± standard error of the mean [SEM] % difference, <12% ± 3.8%).


2005 ◽  
Vol 22 (11-12) ◽  
pp. 379-387 ◽  
Author(s):  
C. Álvarez ◽  
J. A. Castilla ◽  
J. P. Ramírez ◽  
F. Vergara ◽  
A. Yoldi ◽  
...  

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