Neurovascular crosstalk coordinates the central nervous system development

Pax7 is a member of the paired box containing gene family. Its expression pattern suggests a function in cephalic neural crest derivatives, skeletal muscle and central nervous system development. To understand the role of Pax7 during mouse embryogenesis, we used the homologous recombination technique in embryonic stem cells and generated Pax7−/− mice. Homozygous animals are born but die shortly afer weaning. They exhibit malformations in facial structures involving the maxilla and nose. Our analysis suggests that the observed phenotype is due to a cephalic neural crest defect. No obvious phenotype could be detected in the central nervous system and skeletal muscle. Functional redundancy between Pax7 and Pax3 is discussed.

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Expression of Pax6 and Pax7 Proteins During the Central Nervous System Development in Human Embryos

International Journal of Morphology ◽

10.4067/s0717-95022020000200259 ◽

2020 ◽

Vol 38 (2) ◽

pp. 259-264

Author(s):

Aimar Namm ◽

Andres Arend ◽

Siim Suutre ◽

Marina Aunapuu

Keyword(s):

Central Nervous System ◽

Nervous System ◽

System Development ◽

Nervous System Development ◽

Human Embryos ◽

Central Nervous System Development ◽

The Central Nervous System

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Antenatal Glucocorticoids Supplementation and Central Nervous System Development

Current Drug Metabolism ◽

10.2174/1389200211314020002 ◽

2013 ◽

Vol 14 (2) ◽

pp. 160-166

Author(s):

Diego Gazzolo ◽

Laura D. Serpero ◽

Alessandro Frigiola ◽

Raul Abella ◽

Alessandro Giamberti ◽

...

Keyword(s):

Central Nervous System ◽

Nervous System ◽

System Development ◽

Nervous System Development ◽

Central Nervous System Development

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Incorporation of 5-Bromo-2′-deoxyuridine into DNA and Proliferative Behavior of Cerebellar Neuroblasts: All That Glitters Is Not Gold

Cells ◽

10.3390/cells10061453 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1453

Author(s):

Joaquín Martí-Clúa

Keyword(s):

Central Nervous System ◽

Nervous System ◽

System Development ◽

Nervous System Development ◽

Current Data ◽

Cellular Mechanisms ◽

Dividing Cells ◽

The Central Nervous System ◽

Repeated Doses ◽

Pyrimidine Analog

The synthetic halogenated pyrimidine analog, 5-bromo-2′-deoxyuridine (BrdU), is a marker of DNA synthesis. This exogenous nucleoside has generated important insights into the cellular mechanisms of the central nervous system development in a variety of animals including insects, birds, and mammals. Despite this, the detrimental effects of the incorporation of BrdU into DNA on proliferation and viability of different types of cells has been frequently neglected. This review will summarize and present the effects of a pulse of BrdU, at doses ranging from 25 to 300 µg/g, or repeated injections. The latter, following the method of the progressively delayed labeling comprehensive procedure. The prenatal and perinatal development of the cerebellum are studied. These current data have implications for the interpretation of the results obtained by this marker as an index of the generation, migration, and settled pattern of neurons in the developing central nervous system. Caution should be exercised when interpreting the results obtained using BrdU. This is particularly important when high or repeated doses of this agent are injected. I hope that this review sheds light on the effects of this toxic maker. It may be used as a reference for toxicologists and neurobiologists given the broad use of 5-bromo-2′-deoxyuridine to label dividing cells.

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