Phenacoccus madeirensis green (Hemiptera: Pseudococcidae): New geographic records and rapid identification using a species-specific PCR assay

2019 ◽  
Vol 116 ◽  
pp. 68-76 ◽  
Author(s):  
Yu-Sheng Wang ◽  
Tian-Mei Dai ◽  
Hu Tian ◽  
Fang-Hao Wan ◽  
Gui-Fen Zhang
Keyword(s):  
Rapid Identification ◽  
Pcr Assay ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Phenacoccus Madeirensis ◽  
Species Specific

Rapid identification of cervus antlers by species-specific PCR assay

2019 ◽  
Vol 34 (9) ◽  
pp. 1315-1319 ◽  
Author(s):  
Yaya Yang ◽  
Yang Zheng ◽  
Beibei Lu ◽  
Zhaoqun Jiao ◽  
Liqun Chen ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Pcr Assay ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific

Species-specific PCR assay for L. infantum/L. donovani discrimination

Acta Tropica ◽  
2006 ◽  
Vol 100 (3) ◽  
pp. 241-245 ◽  
Author(s):  
Mallorie Hide ◽  
Anne-Laure Bañuls
Keyword(s):  
Pcr Assay ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific

scholarly journals Development of a species-specific PCR assay for identification of the strictly anaerobic bacterium Selenomonas lacticifex found in biofilm-covered surfaces in brewery bottling halls

2014 ◽  
Vol 117 (5) ◽  
pp. 1328-1335 ◽  
Author(s):  
J. Felsberg ◽  
M. Jelínková ◽  
P. Kubizniaková ◽  
D. Matoulková
Keyword(s):  
Anaerobic Bacterium ◽  
Strictly Anaerobic ◽  
Pcr Assay ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific

Development of a Species-Specific PCR Assay for Correct Labeling of Eight Squid Species from Loliginidae and Ommastrephidae Families (Mollusca: Cephalopoda)

2018 ◽  
Vol 11 (11) ◽  
pp. 3071-3077 ◽  
Author(s):  
Hongtao Wang ◽  
Huili Tian ◽  
Na Hao ◽  
Xinqi Wang
Keyword(s):  
Pcr Assay ◽  
Squid Species ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific

Development and validation of a rapid kit for authenticity of murine meat in meat products with a species-specific PCR assay

2020 ◽  
Vol 37 (4) ◽  
pp. 552-560
Author(s):  
Siqi Duan ◽  
Jin Xia Ai ◽  
Liyuan Sun ◽  
Lijun Gao ◽  
Mingcheng Li ◽  
...  
Keyword(s):  
Meat Products ◽  
Pcr Assay ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific ◽  
Development And Validation

scholarly journals Simultaneous Detection and Identification of Common Cell Culture Contaminant and Pathogenic Mollicutes Strains by Reverse Line Blot Hybridization

2004 ◽  
Vol 70 (3) ◽  
pp. 1483-1486 ◽  
Author(s):  
Hui Wang ◽  
Fanrong Kong ◽  
Peter Jelfs ◽  
Gregory James ◽  
Gwendolyn L. Gilbert
Keyword(s):  
Cell Culture ◽  
16S Rrna ◽  
Cell Line ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Pcr Assay ◽  
Intergenic Spacer Region ◽  
Specific Pcr ◽  
Specific Pcr Assay ◽  
Species Specific

ABSTRACT We have developed a reverse line blot (RLB) hybridization assay to detect and identify the commonest mollicutes causing cell line contamination (Mycoplasma arginini, Mycoplasma fermentans, Mycoplasma hyorhinis, Mycoplasma orale, and Acholeplasma laidlawii) and human infection (Mycoplasma pneumoniae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma parvum, and Ureaplasma urealyticum). We developed a nested PCR assay with “universal” primers targeting the mollicute 16S-23S rRNA intergenic spacer region. Amplified biotin-labeled PCR products were hybridized to membrane-bound species-specific oligonucleotide probes. The assay correctly identified reference strains of 10 mollicute species. Cell cultures submitted for detection of mollicute contamination, clinical specimens, and clinical isolates were initially tested by PCR assay targeting a presumed mollicute-specific sequence of the 16S rRNA gene. Any that were positive were assessed by the RLB assay, with species-specific PCR assay as the reference method. Initially, 100 clinical and 88 of 92 cell culture specimens gave concordant results, including 18 in which two or more mollicute species were detected by both methods. PCR and sequencing of the 16S-23S rRNA intergenic spacer region and subsequent retesting by species-specific PCR assay of the four cell culture specimens for which results were initially discrepant confirmed the original RLB results. Sequencing of amplicons from 12 cell culture specimens that were positive in the 16S rRNA PCR assay but negative by both the RLB and species-specific PCR assays failed to identify any mollicute species. The RLB hybridization assay is sensitive and specific and able to rapidly detect and identify mollicute species from clinical and cell line specimens.

scholarly journals Molecular Cloning and Sequencing of thearoA Gene from Actinobacillus pleuropneumoniaeand Its Use in a PCR Assay for Rapid Identification

1999 ◽  
Vol 37 (5) ◽  
pp. 1575-1578 ◽  
Author(s):  
Carmen Hernanz Moral ◽  
Alberto Cascón Soriano ◽  
María Sánchez Salazar ◽  
Javier Yugueros Marcos ◽  
Susana Suárez Ramos ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Chromosomal Dna ◽  
Pcr Assay ◽  
Specific Pcr ◽  
Specificity And Sensitivity ◽  
Pcr Products ◽  
Dna Fragment ◽  
Specific Pcr Assay ◽  
Dna Specificity ◽  
K 12

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5′ and 3′ termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp was amplified from lysed A. pleuropneumoniaeserotypes 1 to 12 of biovar 1 or from isolated DNA. No PCR products were detected when chromosomal DNAs from other genera were used as target DNAs; however, a 1,025-bp DNA fragment was amplified whenActinobacillus equuli chromosomal DNA was used as a target, which could be easily differentiated by its NAD independence. The PCR assay developed was very sensitive, with lower detection limits of 12 CFU with A. pleuropneumoniae cells and 0.8 pg with extracted DNA. Specificity and sensitivity make this PCR assay a useful method for the rapid identification and diagnosis of A. pleuropneumoniae infections.

Rapid and Reliable Identification of Staphylococcus equorum by a Species-Specific PCR Assay Targeting the sodA Gene

2004 ◽  
Vol 27 (6) ◽  
pp. 696-702 ◽  
Author(s):  
Giuseppe Blaiotta ◽  
Danilo Ercolini ◽  
Gianluigi Mauriello ◽  
Giovanni Salzano ◽  
Francesco Villani
Keyword(s):  
Pcr Assay ◽  
Reliable Identification ◽  
Specific Pcr ◽  
Staphylococcus Equorum ◽  
Specific Pcr Assay ◽  
Species Specific
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