Localization and Visualization of Dimethyl Sulfoxide In Mentha X Piperita Shoot Tips

Shoot tips of silver birch (Betulapendula Roth) derived from in vitro cultures originating from mature trees were used as explant material. Several pretreatments prior to cryopreservation were studied in order to enhance shoot tip recovery after storage in liquid nitrogen. Cold acclimation and the classical slow freezing procedure proved to be essential for successful survival, while both nonhardening and vitrification resulted in minimal survival. The optimum procedure was the following: Shoots were cold hardened (5 °C on a 8 h: 16 h (light:dark) cycle) for 3 weeks, and dissected shoot tips were then precultivated on woody plant medium with 5% dimethyl sulfoxide for 72 h. The material was transferred to cryotubes, and a mixture of 10% polyethylene glycol, 10% glucose, and 10% dimethyl sulfoxide was used as cryoprotectant. The cryotubes were slowly cooled to −38 °C before immersion in liquid nitrogen for 8 d. After fast thawing, axillary buds formed shoots without callus formation, but the growth of the new shoots was delayed from a few days to several weeks. Variability in regrowth was considerable as a result of genotype differences.

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Dimethyl sulfoxide as a vehicle for corticosteroids. A comparison with the occlusive dressing technique

Archives of Dermatology ◽

10.1001/archderm.97.2.110 ◽

1968 ◽

Vol 97 (2) ◽

pp. 110-114 ◽

Cited By ~ 2

Author(s):

J. T. Groel

Keyword(s):

Dimethyl Sulfoxide ◽

Occlusive Dressing

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Mentha x piperita L.

Zeitschrift für Komplementärmedizin ◽

10.1055/s-0036-1587997 ◽

2016 ◽

Vol 08 (04) ◽

pp. 50-51

Author(s):

Wolfgang Widmaier

Keyword(s):

Mentha X Piperita

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The Effect of Dimethyl Sulfoxide on In Vitro Platelet Function

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648027 ◽

1976 ◽

Vol 36 (01) ◽

pp. 221-229 ◽

Cited By ~ 16

Author(s):

Charles A. Schiffer ◽

Caroline L. Whitaker ◽

Morton Schmukler ◽

Joseph Aisner ◽

Steven L. Hilbert

Keyword(s):

Platelet Count ◽

Platelet Aggregation ◽

Dimethyl Sulfoxide ◽

Platelet Function ◽

Platelet Volume ◽

Short Term ◽

Platelet Factor ◽

Short Term Exposure ◽

Structural Abnormalities

SummaryAlthough dimethyl sulfoxide (DMSO) has been used extensively as a cryopreservative for platelets there are few studies dealing with the effect of DMSO on platelet function. Using techniques similar to those employed in platelet cryopreservation platelets were incubated with final concentrations of 2-10% DMSO at 25° C. After exposure to 5 and 10% DMSO platelets remained discoid and electron micrographs revealed no structural abnormalities. There was no significant change in platelet count. In terms of injury to platelet membranes, there was no increased availability of platelet factor-3 or leakage of nucleotides, 5 hydroxytryptamine (5HT) or glycosidases with final DMSO concentrations of 2.5, 5 and 10% DMSO. Thrombin stimulated nucleotide and 5HT release was reduced by 10% DMSO. Impairment of thrombin induced glycosidase release was noted at lower DMSO concentrations and was dose related. Similarly, aggregation to ADP was progressively impaired at DMSO concentrations from 1-5% and was dose related. After the platelets exposed to DMSO were washed, however, aggregation and release returned to control values. Platelet aggregation by epinephrine was also inhibited by DMSO and this could not be corrected by washing the platelets. DMSO-plasma solutions are hypertonic but only minimal increases in platelet volume (at 10% DMSO) could be detected. Shrinkage of platelets was seen with hypertonic solutions of sodium chloride or sucrose suggesting that the rapid transmembrane passage of DMSO prevented significant shifts of water. These studies demonstrate that there are minimal irreversible alterations in in vitro platelet function after short-term exposure to DMSO.

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Preservation of Morphological Integrity and Clot Retraction Activity of Human Platelets After Freezing

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654822 ◽

1964 ◽

Vol 11 (01) ◽

pp. 222-229 ◽

Cited By ~ 8

Author(s):

Isaac Djerassi ◽

Albert Roy ◽

Jorge Alvarado ◽

Keyword(s):

Dimethyl Sulfoxide ◽

Liquid Nitrogen ◽

Human Platelets ◽

Slow Freezing ◽

Critical Conditions ◽

Plasma Medium ◽

Clot Retraction ◽

Controlled Freezing ◽

Direct Immersion

SummaryHuman platelets frozen at −195° C (liquid nitrogen) retain their morphological integrity and ability to promote clot retraction when 5% dimethyl-sulfoxide and 5% dextrose are added to the suspending plasma medium. Slow freezing was more effective than direct immersion in the liquid nitrogen. Although similar results may be achieved with dimethylsulfoxide alone with rigidly controlled freezing rates, the addition of sugars may permit freezing under less critical conditions.Dimethylsulfoxyd und 5% Dextrose dem Plasmamilieu hinzugefügt werden. Das langsame Einfrieren ist effektiver als das direkte Eintauchen in flüssigen Stickstoff. Obschon ähnliche Resultate mit Dimethylsulfoxyd allein unter exakter Kontrolle der Einfrierungsgeschwindig-keit erreicht werden können, erlaubt die Zugabe von Dextrose ein Einfrieren unter weniger kritischen Bedingungen.

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