Skład chemiczny i działanie przeciwutleniające ekstraktów z Mentha x piperita L.

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

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Phenolic Profile and Antioxidant Evaluation of Mentha x Piperita L. (Peppermint) Extracts

Natural Product Communications ◽

10.1177/1934578x0900400419 ◽

2009 ◽

Vol 4 (4) ◽

pp. 1934578X0900400 ◽

Cited By ~ 12

Author(s):

H.J. Damien Dorman ◽

Müberra Koşar ◽

K Hüsnü C Başer ◽

Raimo Hiltunen

Keyword(s):

Free Radical ◽

Rosmarinic Acid ◽

High Performance ◽

Radical Scavenging ◽

Antioxidant Property ◽

Free Radical Scavenging ◽

Soluble Extract ◽

Photodiode Array Detection ◽

Mentha X Piperita ◽

Soxhlet Apparatus

Seven extracts were prepared from Mentha x piperita (peppermint) leaves in sequence using a Soxhlet apparatus, viz. (40-60°) light petroleum (PE), dichloromethane (CH2Cl2), acetonitrile (ACN), ethyl acetate (EtOAc), methanol (MeOH), n-butanol and water (H2O) extracts. The phenolic and flavonoid content of each extract were estimated using spectrophotometric methods whilst a qualitative-quantitative analysis was made by reverse-phase high performance liquid chromatography coupled with photodiode array detection (HPLC-PDA). Each extract was assessed in a battery of six antioxidant-related assays so as to determine their iron(III) reductive, iron(II) chelating and free radical scavenging abilities. The MeOH-soluble extract contained the greatest content of total phenols and flavonoids based upon the Folin-Ciocalteu and 2-aminoethyl diphenylborate reagent data and HPLC-PDA analysis. Based upon the chromatographic and UV-spectral data, the leaves principally contained the cinnamic acid caffeic acid, the depside rosmarinic acid and flavonoids (flavones and flavanones). Eriocitrin (383.3 ± 2.2 mg/g extract) and rosmarinic acid (381.2 ± 1.9 mg/g extract) were the most abundant components identified within the leaves, whilst naringenin-7- O-glucoside (0.8 ± 0.01 mg/g extract) was the least abundant component identified being found only in the EtOAc-soluble extract. The EtOAc, ACN and H2O-soluble extracts demonstrated the most potent iron(III) reductive and 1,1′-diphenyl-2-picrylhydrayl, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) and hydroxyl free radical scavenging properties; however, the H2O and CH2Cl2-soluble extracts were the most potent extracts in the β-carotene-linoleic acid bleaching inhibition assay. In terms of iron(II) chelation – an important antioxidant property - the PE, MeOH and H2O extracts demonstrated moderate iron(II) chelating activity.

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Lamiaceae carbohydrates. II. Water-soluble polysaccharides from Mentha x piperita

Chemistry of Natural Compounds ◽

10.1007/s10600-007-0219-6 ◽

2007 ◽

Vol 43 (6) ◽

pp. 648-651 ◽

Cited By ~ 1

Author(s):

D. N. Olennikov ◽

L. M. Tankhaeva

Keyword(s):

Water Soluble ◽

Mentha X Piperita

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Improving Phenolic Total Content and Monoterpene in Mentha x piperita by Using Salicylic Acid or Methyl Jasmonate Combined with Rhizobacteria Inoculation

International Journal of Molecular Sciences ◽

10.3390/ijms21010050 ◽

2019 ◽

Vol 21 (1) ◽

pp. 50 ◽

Cited By ~ 4

Author(s):

Lorena del Rosario Cappellari ◽

Maricel Valeria Santoro ◽

Axel Schmidt ◽

Jonathan Gershenzon ◽

Erika Banchio

Keyword(s):

Salicylic Acid ◽

Plant Species ◽

Management Practice ◽

Total Content ◽

Plant Growth Promoting Rhizobacteria ◽

Total Phenolic ◽

Exogenous Application ◽

Mentha X Piperita ◽

Plant Growth Promoting ◽

Growth Promoting

The effects of plant inoculation with plant growth-promoting rhizobacteria (PGPR) and those resulting from the exogenous application of salicylic acid (SA) or methyl jasmonte (MeJA) on total phenolic content (TPC) and monoterpenes in Mentha x piperita plants were investigated. Although the PGPR inoculation response has been studied for many plant species, the combination of PGPR and exogenous phytohormones has not been investigated in aromatic plant species. The exogenous application of SA produced an increase in TPC that, in general, was of a similar level when applied alone as when combined with PGPR. This increase in TPC was correlated with an increase in the activity of the enzyme phenylalanine ammonia lyase (PAL). Also, the application of MeJA at different concentrations in combination with inoculation with PGPR produced an increase in TPC, which was more relevant at 4 mM, with a synergism effect being observed. With respect to the main monoterpene concentrations present in peppermint essential oil (EO), it was observed that SA or MeJA application produced a significant increase similar to that of the combination with rhizobacteria. However, when plants were exposed to 2 mM MeJA and inoculated, an important increase was produced in the concentration on menthol, pulegone, linalool, limonene, and menthone concentrations. Rhizobacteria inoculation, the treatment with SA and MeJA, and the combination of both were found to affect the amount of the main monoterpenes present in the EO of M. piperita. For this reason, the expressions of genes related to the biosynthesis of monoterpene were evaluated, with this expression being positively affected by MeJA application and PGPR inoculation, but was not modified by SA application. Our results demonstrate that MeJA or SA application combined with inoculation with PGPR constitutes an advantageous management practice for improving the production of secondary metabolites from M. piperita.

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