Development of real-time PCR assay for differential detection of Bordetella bronchiseptica and Bordetella parapertussis

2014 ◽  
Vol 78 (4) ◽  
pp. 347-351 ◽  
Author(s):  
Anette Tizolova ◽  
Delphine Brun ◽  
Nicole Guiso ◽  
Sophie Guillot
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bordetella Bronchiseptica ◽  
Differential Detection ◽  
Pcr Assay ◽  
Bordetella Parapertussis

scholarly journals Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described real-time PCR targeting the pertactin gene

2007 ◽  
Vol 56 (12) ◽  
pp. 1608-1610 ◽  
Author(s):  
Karen B. Register ◽  
Tracy L. Nicholson
Keyword(s):  
Real Time ◽  
Bordetella Pertussis ◽  
Real Time Pcr ◽  
Gene Sequence ◽  
Significant Proportion ◽  
Bordetella Bronchiseptica ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Avian Origin ◽  
Pcr Targeting

Recently, a real-time PCR (RT-PCR) assay based on sequence from the gene for pertactin was proposed for identification of Bordetella pertussis. Here, it is reported that the B. pertussis pertactin gene sequence for the region that encompasses the RT-PCR probe and primers is nearly identical to that of many Bordetella bronchiseptica strains of human and avian origin. Additionally, it is demonstrated that such strains are erroneously identified as B. pertussis using the RT-PCR assay. These data suggest that the use of the assay without confirmatory testing may result in erroneous identification of a significant proportion of human isolates of B. bronchiseptica as B. pertussis.

scholarly journals A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

2018 ◽  
Vol 15 (1) ◽  
Author(s):  
Fang-zhou Qiu ◽  
Xin-xin Shen ◽  
Meng-chuan Zhao ◽  
Li Zhao ◽  
Su-xia Duan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Human Adenovirus ◽  
Differential Detection ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay

Triplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis

Apmis ◽  
2010 ◽  
Vol 118 (9) ◽  
pp. 685-691 ◽  
Author(s):  
YINGHUA XU ◽  
YUNQIANG XU ◽  
QIMING HOU ◽  
RUIFU YANG ◽  
SHUMIN ZHANG
Keyword(s):  
Real Time ◽  
Bordetella Pertussis ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Bordetella Parapertussis
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