Triplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis

Apmis ◽  
2010 ◽  
Vol 118 (9) ◽  
pp. 685-691 ◽  
Author(s):  
YINGHUA XU ◽  
YUNQIANG XU ◽  
QIMING HOU ◽  
RUIFU YANG ◽  
SHUMIN ZHANG
Keyword(s):  
Real Time ◽  
Bordetella Pertussis ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Bordetella Parapertussis

scholarly journals Addition of a Homologous Internal Control to a Real-Time PCR Assay for Detection of Bordetella pertussis

2005 ◽  
Vol 51 (12) ◽  
pp. 2404-2406 ◽  
Author(s):  
Christoph Koidl ◽  
Michael Bozic ◽  
Jörg Berg ◽  
Markus Stöcher ◽  
Gerhard Mühlbauer ◽  
...  
Keyword(s):  
Real Time ◽  
Bordetella Pertussis ◽  
Internal Control ◽  
Real Time Pcr ◽  
Pcr Assay

scholarly journals Evaluation of Real-Time PCR for Detection of and Discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for Clinical Diagnosis

2003 ◽  
Vol 41 (9) ◽  
pp. 4121-4126 ◽  
Author(s):  
K. E. Templeton ◽  
S. A. Scheltinga ◽  
A. van der Zee ◽  
B. M. W. Diederen ◽  
A. M. Kruijssen ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Diagnosis ◽  
Bordetella Pertussis ◽  
Real Time Pcr ◽  
Bordetella Parapertussis

scholarly journals Evaluation of a real-time PCR assay for detection of Bordetella pertussis and B. parapertussis in clinical samples

2001 ◽  
Vol 50 (5) ◽  
pp. 436-440 ◽  
Author(s):  
KATRIN KÖSTERS ◽  
MARION RIFFELMANN ◽  
CARL HEINZ WIRSING VON KÖNIG
Keyword(s):  
Real Time ◽  
Bordetella Pertussis ◽  
Real Time Pcr ◽  
Clinical Samples ◽  
Pcr Assay

scholarly journals Development of a real-time PCR for the identification of Bordetella pertussis and Bordetella parapertussis

2007 ◽  
Vol 13 (4) ◽  
pp. 419-423 ◽  
Author(s):  
A. Ménard ◽  
P. Lehours ◽  
J. Sarlangue ◽  
C. Bébéar ◽  
F. Mégraud ◽  
...  
Keyword(s):  
Real Time ◽  
Bordetella Pertussis ◽  
Real Time Pcr ◽  
Bordetella Parapertussis

scholarly journals Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described real-time PCR targeting the pertactin gene

2007 ◽  
Vol 56 (12) ◽  
pp. 1608-1610 ◽  
Author(s):  
Karen B. Register ◽  
Tracy L. Nicholson
Keyword(s):  
Real Time ◽  
Bordetella Pertussis ◽  
Real Time Pcr ◽  
Gene Sequence ◽  
Significant Proportion ◽  
Bordetella Bronchiseptica ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Avian Origin ◽  
Pcr Targeting

Recently, a real-time PCR (RT-PCR) assay based on sequence from the gene for pertactin was proposed for identification of Bordetella pertussis. Here, it is reported that the B. pertussis pertactin gene sequence for the region that encompasses the RT-PCR probe and primers is nearly identical to that of many Bordetella bronchiseptica strains of human and avian origin. Additionally, it is demonstrated that such strains are erroneously identified as B. pertussis using the RT-PCR assay. These data suggest that the use of the assay without confirmatory testing may result in erroneous identification of a significant proportion of human isolates of B. bronchiseptica as B. pertussis.

scholarly journals Real-time PCR-based detection of Bordetella pertussis and Bordetella parapertussis in an Irish paediatric population

2011 ◽  
Vol 60 (6) ◽  
pp. 722-729 ◽  
Author(s):  
Juanita A. Grogan ◽  
Catriona Logan ◽  
John O’Leary ◽  
Rebecca Rush ◽  
Niamh O’Sullivan
Keyword(s):  
Real Time ◽  
Bordetella Pertussis ◽  
Real Time Pcr ◽  
Paediatric Population ◽  
Bordetella Parapertussis
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