scholarly journals Spore photoproduct within DNA is a surprisingly poor substrate for its designated repair enzyme—The spore photoproduct lyase

DNA Repair ◽  
2017 ◽  
Vol 53 ◽  
pp. 31-42 ◽  
Author(s):  
Linlin Yang ◽  
Yajun Jian ◽  
Peter Setlow ◽  
Lei Li
Keyword(s):  
Repair Enzyme ◽  
Spore Photoproduct Lyase ◽  
Spore Photoproduct

scholarly journals The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition

2014 ◽  
Vol 70 (3) ◽  
pp. 752-759 ◽  
Author(s):  
Isha Singh ◽  
Yajun Lian ◽  
Lei Li ◽  
Millie M. Georgiadis
Keyword(s):  
Structural Changes ◽  
Leukemia Virus ◽  
Structural Features ◽  
Moloney Murine Leukemia Virus ◽  
Complementary Strand ◽  
Repair Enzyme ◽  
Independent Manner ◽  
Spore Photoproduct Lyase ◽  
Local Conformation ◽  
Spore Photoproduct

The spore photoproduct lesion (SP; 5-thymine-5,6-dihydrothymine) is the dominant photoproduct found in UV-irradiated spores of some bacteria such asBacillus subtilis. Upon spore germination, this lesion is repaired in a light-independent manner by a specific repair enzyme: the spore photoproduct lyase (SP lyase). In this work, a host–guest approach in which the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase (MMLV RT) serves as the host and DNA as the guest was used to determine the crystal structures of complexes including 16 bp oligonucleotides with and without the SP lesion at 2.14 and 1.72 Å resolution, respectively. In contrast to other types of thymine–thymine lesions, the SP lesion retains normal Watson–Crick hydrogen bonding to the adenine bases of the complementary strand, with shorter hydrogen bonds than found in the structure of the undamaged DNA. However, the lesion induces structural changes in the local conformation of what is otherwise B-form DNA. The region surrounding the lesion differs significantly in helical form from B-DNA, and the minor groove is widened by almost 3 Å compared with that of the undamaged DNA. Thus, these unusual structural features associated with SP lesions may provide a basis for recognition by the SP lyase.

scholarly journals Solution phase dynamics of the DNA repair enzyme spore photoproduct lyase as probed by H/D exchange

FEBS Letters ◽  
2014 ◽  
Vol 588 (17) ◽  
pp. 3023-3029 ◽  
Author(s):  
Shourjo Ghose ◽  
Jonathan K. Hilmer ◽  
Brian Bothner ◽  
Joan B. Broderick
Keyword(s):  
Dna Repair ◽  
Solution Phase ◽  
Repair Enzyme ◽  
Phase Dynamics ◽  
Spore Photoproduct Lyase ◽  
Spore Photoproduct

scholarly journals Characterization of an Active Spore Photoproduct Lyase, a DNA Repair Enzyme in the RadicalS-Adenosylmethionine Superfamily

2006 ◽  
Vol 281 (36) ◽  
pp. 25994-26003 ◽  
Author(s):  
Jeffrey M. Buis ◽  
Jennifer Cheek ◽  
Efthalia Kalliri ◽  
Joan B. Broderick
Keyword(s):  
Dna Repair ◽  
Repair Enzyme ◽  
Spore Photoproduct Lyase ◽  
Spore Photoproduct

Characterization of the DNA repair spore photoproduct lyase enzyme from Clostridium acetobutylicum: A radical-SAM enzyme

2007 ◽  
Vol 10 (8) ◽  
pp. 756-765 ◽  
Author(s):  
Alexia Chandor ◽  
Thierry Douki ◽  
Didier Gasparutto ◽  
Serge Gambarelli ◽  
Yannis Sanakis ◽  
...  
Keyword(s):  
Dna Repair ◽  
Clostridium Acetobutylicum ◽  
Radical Sam ◽  
Spore Photoproduct Lyase ◽  
Radical Sam Enzyme ◽  
Spore Photoproduct

scholarly journals Rescuing DNA repair activity by rewiring the H-atom transfer pathway in the radical SAM enzyme, spore photoproduct lyase

2014 ◽  
Vol 50 (91) ◽  
pp. 14201-14204 ◽  
Author(s):  
Alhosna Benjdia ◽  
Korbinian Heil ◽  
Andreas Winkler ◽  
Thomas Carell ◽  
Ilme Schlichting
Keyword(s):  
Dna Repair ◽  
Atom Transfer ◽  
Radical Sam ◽  
Spore Photoproduct Lyase ◽  
Repair Activity ◽  
Radical Sam Enzyme ◽  
Spore Photoproduct

scholarly journals Dinucleotide Spore Photoproduct, a Minimal Substrate of the DNA Repair Spore Photoproduct Lyase Enzyme fromBacillus subtilis

2006 ◽  
Vol 281 (37) ◽  
pp. 26922-26931 ◽  
Author(s):  
Alexia Chandor ◽  
Olivier Berteau ◽  
Thierry Douki ◽  
Didier Gasparutto ◽  
Yannis Sanakis ◽  
...  
Keyword(s):  
Dna Repair ◽  
Spore Photoproduct Lyase ◽  
Spore Photoproduct
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