Heavy metal contamination to Poyang Lake is increasingly severe. Bellamya aeruginosas, the dominant species of bellamya in Poyang Lake, was chosen in this project to carry out a proteomics study, because they obtain nutrients from sediments directly and have a strong heavy metal enrichment capability. To get a better knowledge of the contamination level of Poyang Lake and a better understanding of toxicity and bioavailability of heavy metals contamination, the main heavy metals of surface sediments were tested and a proteomics study was carried out on bellamya aeruginosa, the dominant species of bellamya in Poyang Lake. Results of heavy metal tests show, to Poyang Lake, the most contaminated heavy metal is Cu, the widest contaminated heavy metal is Pb, the most contaminated area is the south lake area, and the least contaminated area is the entrance of Xioushui River. Proteomics analysis shows the main significantly different proteins are keratins or similar substances. These proteins are higher expressed in the samples obtained from the most contaminated area, so they could be the responding biomarkers for monitoring heavy metal contamination of surface sediment in Poyang Lake.