Antidiabetic activity of some pentacyclic acid triterpenoids, role of PTP–1B: In vitro, in silico, and in vivo approaches

Ruellia tuberosaL. is a folk remedy in the treatment of diabetes mellitus. However, its hypoglycemic activity has not been investigated so far. In the present study, the antidiabetic mechanism of the n-hexane fraction of methanolic extract (HFME) of this plant was investigatedin silico,in vitro, andin vivo.In silicostudy was performed using AutoDock4.2 software.In vitro α-amylase inhibitory activity was investigated by starch-iodine method. A single dose of 450 mg/kg HFME for 14 days was subjected to an antidiabetic screeningin vivoby a multiple low dose streptozotocin (MLD-STZ) induced rats. Molecular modeling results show that Betulin exhibited noncompetitiveα-amylase inhibitory activities. The effect of HFME elicited significant reductions of diabetic rat blood glucose. A single dose administration of HFME inhibitedα-amylase activityin vivo(P<0.01) compared to a diabetic control group. Moreover, this extract strongly inhibited theα-amylase activityin vitro(IC500.14 ± 0.005 mg/mL). It is concluded that HFME exerted an antidiabetic effect viaα-amylase inhibitor. Our findings provide a possible hypoglycemic action ofR. tuberosaL. as an alternative therapy in the management of diabetes.

Download Full-text

Synthesis of oleanolic acid derivatives: In vitro, in vivo and in silico studies for PTP-1B inhibition

European Journal of Medicinal Chemistry ◽

10.1016/j.ejmech.2014.09.036 ◽

2014 ◽

Vol 87 ◽

pp. 316-327 ◽

Cited By ~ 20

Author(s):

Juan José Ramírez-Espinosa ◽

Maria Yolanda Rios ◽

Paolo Paoli ◽

Virginia Flores-Morales ◽

Guido Camici ◽

...

Keyword(s):

Oleanolic Acid ◽

In Silico ◽

In Silico Studies ◽

Ptp 1B

Download Full-text

In Silico Experiments in Scientific Papers on Molecular Biology

Science & Technology Studies ◽

10.23987/sts.55262 ◽

2011 ◽

Vol 24 (2) ◽

pp. 23-42

Author(s):

Sabrina Moretti

Keyword(s):

Molecular Biology ◽

In Silico ◽

In Vitro Experiments ◽

Technical Object ◽

Experimental Conditions ◽

Biological Phenomena ◽

Scientific Papers

This article explores the role of the so-called in silico experiments used in molecular biology. It is based on the analysis of some papers that present scientific applications which rely on in silico experiments. By means of this study I found two basic ways of viewing them. According to the first view, the in silico experiment is a computer program that realizes some specific operations: it constitutes some particular experimental conditions, which allow us to investigate biological phenomena, and which complement those present in in vivo and in vitro experiments. According to the second view, in silico experimentation has a different meaning, which corresponds more closely to the meaning of “simulation”: its identity is linked to that of the “model” used to construct such simulation. The authors of the analysed papers never express an intention to standardize a model, so its meaning remains contingent, and cannot be turned into a technical object.

Download Full-text