Dissolved Metal (Fe, Mn, Zn, Ni, Cu, Co, Cd, Pb) and Metalloid (As, Sb) in Snow Water across a 2800 km Latitudinal Profile of Western Siberia: Impact of Local Pollution and Global Transfer

Snow cover is known to be an efficient and unique natural archive of atmospheric input and an indicator of ecosystem status. In high latitude regions, thawing of snow provides a sizable contribution of dissolved trace metals to the hydrological network. Towards a better understanding of natural and anthropogenic control on heavy metals and metalloid input from the atmosphere to the inland waters of Siberian arctic and subarctic regions, we measured chemical composition of dissolved (<0.22 µm) fractions of snow across a 2800 km south–north gradient in Western Siberia. Iron, Mn, Co, Ni, and Cd demonstrated sizable (by a factor of 4–7) decrease in concentration northward, which can be explained by a decrease in overall population density and the influence of dry aerosol deposition. Many elements (Mn, Ni, Cu, Cd, Pb, As, and Sb) exhibited a prominent local maximum (a factor of 2–3) in the zone of intensive oil and gas extraction (61–62° N latitudinal belt), which can be linked to gas flaring and fly ash deposition. Overall, the snow water chemical composition reflected both local and global (long-range) atmospheric transfer processes. Based on mass balance calculation, we demonstrate that the winter time atmospheric input represents sizable contribution to the riverine export fluxes of dissolved (<0.45 µm) Mn, Co, Zn, Cd, Pb, and Sb during springtime and can appreciably shape the hydrochemical composition of the Ob River main stem and tributaries.

Aerosol tracer testing in Boeing 767 and 777 aircraft to simulate exposure potential of infectious aerosol such as SARS-CoV-2

The COVID-19 pandemic has reintroduced questions regarding the potential risk of SARS-CoV-2 exposure amongst passengers on an aircraft. Quantifying risk with computational fluid dynamics models or contact tracing methods alone is challenging, as experimental results for inflight biological aerosols is lacking. Using fluorescent aerosol tracers and real time optical sensors, coupled with DNA-tagged tracers for aerosol deposition, we executed ground and inflight testing on Boeing 767 and 777 airframes. Analysis here represents tracer particles released from a simulated infected passenger, in multiple rows and seats, to determine the exposure risk via penetration into breathing zones in that row and numerous rows ahead and behind the index case. We present here conclusions from 118 releases of fluorescent tracer particles, with 40+ Instantaneous Biological Analyzer and Collector sensors placed in passenger breathing zones for real-time measurement of simulated virus particle penetration. Results from both airframes showed a minimum reduction of 99.54% of 1 μm aerosols from the index source to the breathing zone of a typical passenger seated directly next to the source. An average 99.97 to 99.98% reduction was measured for the breathing zones tested in the 767 and 777, respectively. Contamination of surfaces from aerosol sources was minimal, and DNA-tagged 3 μm tracer aerosol collection techniques agreed with fluorescent methodologies.

Numerical Investigation of Bioaerosol Transport in a Compact Lavatory

The lavatory is a fertile area for the transmission of infectious disease through bioaerosols between its users. In this study, we built a generic compact lavatory model with a vacuum toilet, and computational fluid dynamics (CFD) is used to evaluate the effects of ventilation and user behaviors on the airflow patterns, and the resulting fates of bioaerosols. Fecal aerosols are readily released into the lavatory during toilet flush. Their concentration rapidly decays in the first 20 s after flushing by deposition or dilution. It takes about 315 s to 348 s for fine bioaerosols (<10 µm in diameter) to decrease to 5% of the initial concentration, while it takes 50 and 100 µm bioaerosols approximately 11 and <1 s, respectively, to completely deposit. The most contaminated surfaces by aerosol deposition include the toilet seat, the bowl, and the nearby walls. The 10 µm aerosols tend to deposit on horizontal surfaces, while the 50 and 100 µm bioaerosols almost always deposit on the bowl. In the presence of a standing thermal manikin, the rising thermal plume alters the flow field and more bioaerosols are carried out from the toilet; a large fraction of aerosols deposit on the manikin’s legs. The respiratory droplets generated by a seated coughing manikin tend to deposit on the floor, legs, and feet of the manikin. In summary, this study reveals the bioaerosol dilution time and the easily contaminated surfaces in a compact lavatory, which will aid the development of control measures against infectious diseases.

Development of a rat capnoperitoneum phantom to study drug aerosol deposition in the context of anticancer research on peritoneal carcinomatosis

Pressurized Intraperitoneal Aerosol Chemotherapy (PIPAC) is a promising approach with a high optimization potential for the treatment of peritoneal carcinomatosis. To study the efficacy of PIPAC and drugs, first rodent cancer models were developed. But inefficient drug aerosol supply and knowledge gaps concerning spatial drug distribution can limit the results based on such models. To study drug aerosol supply/deposition, computed tomography scans of a rat capnoperitoneum were used to deduce a virtual and a physical phantom of the rat capnoperitoneum (RCP). RCP qualification was performed for a specific PIPAC method, where the capnoperitoneum is continuously purged by the drug aerosol. In this context, also in-silico analyses by computational fluid dynamic modelling were conducted on the virtual RCP. The physical RCP was used for ex-vivo granulometric analyses concerning drug deposition. Results of RCP qualification show that aerosol deposition in a continuous purged rat capnoperitoneum depends strongly on the position of the inlet and outlet port. Moreover, it could be shown that the droplet size and charge condition of the drug aerosol define the deposition efficiency. In summary, the developed virtual and physical RCP enables detailed in-silico and ex-vivo analyses on drug supply/deposition in rodents.