Construction of an amperometric pyruvate oxidase enzyme electrode for determination of pyruvate and phosphate

2007 ◽  
Vol 52 (28) ◽  
pp. 7972-7977 ◽  
Author(s):  
Erol Akyilmaz ◽  
Emine Yorganci
Keyword(s):  
Enzyme Electrode ◽  
Pyruvate Oxidase ◽  
Oxidase Enzyme

A Novel Catechol Oxidase Enzyme Electrode for the Specific Determination of Catechol

1998 ◽  
Vol 62 (11) ◽  
pp. 2098-2100 ◽  
Author(s):  
Erhan DINÇKAYA ◽  
Erol AKYILMAZ ◽  
Sinan AKGÖL ◽  
Seçil TATAR ÖNAL ◽  
Figen ZIHNIOGLU ◽  
...  
Keyword(s):  
Enzyme Electrode ◽  
Catechol Oxidase ◽  
Specific Determination ◽  
Oxidase Enzyme

Determination of sulfite in food products by an enzyme electrode

1991 ◽  
Vol 18 (1-2) ◽  
pp. 93-102 ◽  
Author(s):  
A. Mulchandani ◽  
C.A. Groom ◽  
J.H.T. Luong
Keyword(s):  
Food Products ◽  
Enzyme Electrode

Enzyme electrode for the determination of glucose

1981 ◽  
Vol 53 (1) ◽  
pp. 51-53 ◽  
Author(s):  
Esther. Lobel ◽  
Judith. Rishpon
Keyword(s):  
Enzyme Electrode

Enzymatische und nicht-enzymatische Darstellung von „Thiaminpyrophosphataktiviertem Glykolaldehyd“

1967 ◽  
Vol 22 (4) ◽  
pp. 407-412 ◽  
Author(s):  
F. Pohlandt ◽  
G. Kohlhaw ◽  
H. Holzer
Keyword(s):  
Ion Exchange ◽  
Magnesium Chloride ◽  
Enzymatic Reaction ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Thiamine Pyrophosphate ◽  
Pyruvate Oxidase ◽  
Great Excess

1. A reaction mixture, made from pig heart pyruvate oxidase, hydroxypyruvate, thiamine pyrophosphate, and magnesium chloride, is described in which thiamine pyrophosphate is almost quantitatively converted into 2-(1,2-dihydroxyethyl)-thiamine pyrophosphate (thiamine pyrophosphate-activated glycolaldehyde). This product can be isolated by ion exchange chromatography.2. 2-(1,2-dihydroxyethyl)-thiamine pyrophosphate can also be isolated from reaction mixtures of yeast transketolase with hydroxypyruvate, thiamine pyrophosphate, and magnesium chloride, but in very low yield.3. From non-enzymatic reaction mixtures containing thiamine pyrophosphate and a great excess of glycolaldehyde, a compound could be isolated, the tested properties of which were found to be the same as those of enzymatically prepared 2-(1,2-dihydroxyethyl)-thiamine pyrophosphate.4. 2-(1,2-dihydroxyethyl)-thiamine pyrophosphate gives rise to only 55% of the thiochrome which is produced from the same quantity of thiamine pyrophosphate. This behaviour can be used for the determination of relative concentrations of thiamine pyrophosphate and 2-(1,2-dihydroxyethyl)-thiamine pyrophosphate in mixtures.

Determination of total protein in serum using a tyrosinase enzyme electrode

1985 ◽  
Vol 57 (9) ◽  
pp. 1925-1928 ◽  
Author(s):  
Takashi. Toyota ◽  
Shia S. Kuan ◽  
George G. Guilbault
Keyword(s):  
Total Protein ◽  
Enzyme Electrode ◽  
Tyrosinase Enzyme
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