Genetic engineering of glycinebetaine synthesis enhances cadmium tolerance in BADH-transgenic tobacco plants via reducing cadmium uptake and alleviating cadmium stress damage

2021 ◽  
pp. 104602
Author(s):  
Chongyang Li ◽  
Tianpeng Zhang ◽  
Pengwen Feng ◽  
Daxing Li ◽  
Marian Brestic ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Transgenic Tobacco ◽  
Cadmium Stress ◽  
Cadmium Uptake ◽  
Cadmium Tolerance ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Stress Damage

Over-Expression of AtEXPB2 Alleviates Salt Stress Damage in Transgenic Tobacco Plants

2021 ◽  
Vol 68 (2) ◽  
pp. 274-285
Author(s):  
M. Chalekaei ◽  
A. Abbasi ◽  
S. Yousefi ◽  
D. Dadashi
Keyword(s):  
Salt Stress ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Over Expression ◽  
Stress Damage

Functional components of the bacterial CzcCBA efflux system reduce cadmium uptake and accumulation in transgenic tobacco plants

2017 ◽  
Vol 35 ◽  
pp. 54-61 ◽  
Author(s):  
Andrea Nesler ◽  
Giovanni DalCorso ◽  
Elisa Fasani ◽  
Anna Manara ◽  
Gian Pietro Di Sansebastiano ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cadmium Uptake ◽  
Transgenic Tobacco Plants ◽  
Efflux System ◽  
Tobacco Plants ◽  
Functional Components

Transgenic tobacco plants overproducing alfalfa aldose/aldehyde reductase show higher tolerance to low temperature and cadmium stress

Plant Science ◽  
2004 ◽  
Vol 166 (5) ◽  
pp. 1329-1333 ◽  
Author(s):  
Attila Hegedüs ◽  
Sára Erdei ◽  
Tibor Janda ◽  
Erika Tóth ◽  
Gábor Horváth ◽  
...  
Keyword(s):  
Low Temperature ◽  
Transgenic Tobacco ◽  
Cadmium Stress ◽  
Aldehyde Reductase ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

Cadmium tolerance and accumulation in transgenic tobacco plants with a yeast metallothionein combined with a polyhistidine tail

2004 ◽  
Vol 54 (2-3) ◽  
pp. 233-237 ◽  
Author(s):  
Daniela Pavlı́ková ◽  
Tomas Macek ◽  
Martina Macková ◽  
Jirina Száková ◽  
Jiri Balı́k
Keyword(s):  
Transgenic Tobacco ◽  
Cadmium Tolerance ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells

2005 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Helga Schinkel ◽  
Andreas Schiermeyer ◽  
Raphael Soeur ◽  
Rainer Fischer ◽  
Stefan Schillberg
Keyword(s):  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Suspension Cells ◽  
Tobacco Plants ◽  
Recombinant Thrombomodulin

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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