Over-Expression of AtEXPB2 Alleviates Salt Stress Damage in Transgenic Tobacco Plants

2021 ◽  
Vol 68 (2) ◽  
pp. 274-285
Author(s):  
M. Chalekaei ◽  
A. Abbasi ◽  
S. Yousefi ◽  
D. Dadashi
Keyword(s):  
Salt Stress ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Over Expression ◽  
Stress Damage

Genetic engineering of the biosynthesis of glycinebetaine leads to increased tolerance of photosynthesis to salt stress in transgenic tobacco plants

2007 ◽  
Vol 66 (1-2) ◽  
pp. 73-86 ◽  
Author(s):  
Xinghong Yang ◽  
Zheng Liang ◽  
Xiaogang Wen ◽  
Congming Lu
Keyword(s):  
Salt Stress ◽  
Genetic Engineering ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

scholarly journals Overexpression of Grapevine VvIAA18 Gene Enhanced Salt Tolerance in Tobacco

2020 ◽  
Vol 21 (4) ◽  
pp. 1323 ◽  
Author(s):  
Wei Li ◽  
Changxi Dang ◽  
Yuxiu Ye ◽  
Zunxin Wang ◽  
Laibao Hu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Transcriptional Activation ◽  
Late Embryogenesis Abundant Protein ◽  
Pcr Analysis ◽  
Vitis Vinifera L ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

In plants, auxin/indoleacetic acid (Aux/IAA) proteins are transcriptional regulators that regulate developmental process and responses to phytohormones and stress treatments. However, the regulatory functions of the Vitis vinifera L. (grapevine) Aux/IAA transcription factor gene VvIAA18 have not been reported. In this study, the VvIAA18 gene was successfully cloned from grapevine. Subcellular localization analysis in onion epidermal cells indicated that VvIAA18 was localized to the nucleus. Expression analysis in yeast showed that the full length of VvIAA18 exhibited transcriptional activation. Salt tolerance in transgenic tobacco plants and Escherichia. coli was significantly enhanced by VvIAA18 overexpression. Real-time quantitative PCR analysis showed that overexpression of VvIAA18 up-regulated the salt stress-responsive genes, including pyrroline-5-carboxylate synthase (NtP5CS), late embryogenesis abundant protein (NtLEA5), superoxide dismutase (NtSOD), and peroxidase (NtPOD) genes, under salt stress. Enzymatic analyses found that the transgenic plants had higher SOD and POD activities under salt stress. Meanwhile, component analysis showed that the content of proline in transgenic plants increased significantly, while the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) decreased significantly. Based on the above results, the VvIAA18 gene is related to improving the salt tolerance of transgenic tobacco plants. The VvIAA18 gene has the potential to be applied to enhance plant tolerance to abiotic stress.

Over-expression of SlJA2 decreased heat tolerance of transgenic tobacco plants via salicylic acid pathway

2017 ◽  
Vol 36 (4) ◽  
pp. 529-542 ◽  
Author(s):  
Zhong-Ming Liu ◽  
Meng-Meng Yue ◽  
Dong-Yue Yang ◽  
Shao-Bo Zhu ◽  
Na-Na Ma ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Transgenic Tobacco ◽  
Heat Tolerance ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Over Expression ◽  
Salicylic Acid Pathway ◽  
Acid Pathway

scholarly journals Effects of salt stress on proline content, expression of delta-1-pyrroline-5-carboxylate synthetase, and activities of catalase and ascorbate peroxidase in transgenic tobacco plants

2009 ◽  
Vol 46 (2) ◽  
pp. 63-75 ◽  
Author(s):  
Roya Razavizadeh ◽  
Ali Ehsanpour
Keyword(s):  
Salt Stress ◽  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Expression Pattern ◽  
Ascorbate Peroxidase ◽  
Proline Content ◽  
35S Promoter ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Moth Bean

Effects of salt stress on proline content, expression of delta-1-pyrroline-5-carboxylate synthetase, and activities of catalase and ascorbate peroxidase in transgenic tobacco plantsIn arid and semiarid regions, soil salinity limits crop production. Proline accumulation in transgenic plants results in increased stress tolerance, but the underlying mechanism was unclear. To elucidate it, effects of salt stress on the expression pattern of Δ1-pyrroline-5-carboxylate synthetase (P5CS), proline content, catalase (CAT), and ascorbate peroxidase (APX) activities were analyzed in transgenic tobacco (Nicotiana tabacumcv. Wisconsin). Transgenic tobacco plants containing CaMV 35S promoter and theP5CSgene from moth bean (Vigna aconitifolia), linked to theNPTIIgene, were culturedin vitrowith or without 300 mM NaCl. The expression pattern ofP5CSwas evaluated using semiquantitative RT-PCR (reverse transcription-polymerase chain reaction). Time-course experiments showed an increase in proline content after 4 h of the treatment. The level ofP5CStranscripts was increased significantly in leaves and roots of transgenic plants after 24 and 48 h of treatment. This rise in transcripts was concomitant with the highest increase in proline content. In addition, CAT and APX activities increased under salt stress, and their highest activities were observed after 24 and 48 h of NaCl treatment. These results suggest thatP5CSis an inducible gene regulating the activities of CAT and APX and the accumulation of proline in plants subjected to salt stress.

scholarly journals An Arabidopsis Mitochondrial Uncoupling Protein Confers Tolerance to Drought and Salt Stress in Transgenic Tobacco Plants

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23776 ◽  
Author(s):  
Kevin Begcy ◽  
Eduardo D. Mariano ◽  
Lucia Mattiello ◽  
Alessandra V. Nunes ◽  
Paulo Mazzafera ◽  
...  
Keyword(s):  
Salt Stress ◽  
Transgenic Tobacco ◽  
Uncoupling Protein ◽  
Transgenic Tobacco Plants ◽  
Mitochondrial Uncoupling ◽  
Tobacco Plants ◽  
Mitochondrial Uncoupling Protein ◽  
Drought And Salt Stress

Molecular Characterization of a Tonoplast Na+/H+ Antiporter from Iris Lactea

2018 ◽  
Author(s):  
Qiang Guo ◽  
Xiaoxia Tian ◽  
Peichun Mao ◽  
Lin Meng
Keyword(s):  
Salt Stress ◽  
Transgenic Tobacco ◽  
Membrane Integrity ◽  
Nacl Stress ◽  
Proton Pumps ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Cell Membrane Integrity ◽  
Effective Strategies ◽  
Photosynthesis Efficiency

Na+ compartmentalization into vacuoles is one of the effective strategies for adaptation of halophytes to saline environments. The tonoplast Na+/H+ antiporter (NHX) has been proved to be involved in the compartmentalization of Na+ into vacuoles to alleviate Na+ toxicity in cytoplasm. However, the function of NHX in halophyte Iris lactea is still unclear under salt stress. In this study, a significant positive correlation was observed between Na+ accumulations and IlNHX expression levels in shoots and roots under different concentrations of NaCl (0-200 mM), indicating IlNHX might be involved in Na+ accumulation of I. lactea in response to salt stress. More important, IlNHX was specifically localized to the tonoplast. Transgenic tobacco plants expressing IlNHX grew better and showed higher salinity tolerance under salt (200 mM NaCl) stress than those of wild type (WT) plants. Compared to WT plants, transgenic tobacco plants accumulated more Na+ and K+ and maintained higher K+/Na+ ratios in tissues by salt stress, accompanied by the reduction of chlorophyll loss and lipid peroxidation in the presence of salt. Interestingly, we found that transgenic tobacco plants exhibited markedly higher tonoplast H+-ATPase activity relative to WT plants subjected to salt. Overall, overexpression of IlNHX in tobacco could compartmentalize excessive Na+ into vacuoles to keep the cytosolic K+/Na+ balance by enhanced tonoplast proton pumps activity, which would be contributed to maintain K+ and Na+ homeostasis, to improve photosynthesis efficiency and to protect cell membrane integrity under salt stress.

Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations

2004 ◽  
Vol 42 (9) ◽  
pp. 731-738 ◽  
Author(s):  
Ken Kobayashi ◽  
Catherine Sarrobert ◽  
Ximena Ares ◽  
María M. Rivero ◽  
Sara Maldonado ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Potato Virus ◽  
Movement Protein ◽  
Potato Virus X ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Metabolic Alterations ◽  
Over Expression

Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection

2011 ◽  
Vol 49 (11) ◽  
pp. 1264-1271 ◽  
Author(s):  
Chuanfeng Zhu ◽  
Yixue Wang ◽  
Yuanbao Li ◽  
Khizar Hayat Bhatti ◽  
Yingchuan Tian ◽  
...  
Keyword(s):  
Salt Stress ◽  
Transgenic Tobacco ◽  
Pseudomonas Syringae ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants
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